We narrowed to 183 results for: tTA

... Shlomai A, Cox DB, Schwartz RE, Michailidis E, Bhatta A, Scott DA, Zhang F, Rice CM, Bhatia SN. CRISPR...

...Mireault AA, McAnally JR, Li H, Sanchez-Ortiz E, Bhattacharyya S, Shelton JM, Bassel-Duby R, Olson EN. Postnatal...

... causing proteins to migrate out of the gel and attach to the membrane.  In a wet transfer system the ...

...for two-photon (2P) imaging and loose-seal, cell-attached recordings in primary visual cortex (V1) layer...

...highlighted recently it has become more difficult to attain a permanent position in academia. It is therefore...

...Link opens in a new window) Tovote P, Esposito MS, Botta P, Chaudun F, Fadok JP, Markovic M, Wolff SB, Ramakrishnan...

...wreaking havoc in a theme park.  Released in 1997, GATTACA is an American SciFi film starring Ethan Hawke,...

...entire plate was scanned for V5 tagging using an Operetta imaging system. Dewari et al, eLife 2018;7:e35069...

...resulting in site-specific incorporation of the attached fluorophore. However, since tRNA suppression competes...

... AAV construct is limited to only a few kb so attaining neuronal specificity through promoter-directed...

...(6096):816-21. PMID: 22745249 Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, Joung...

... L, Heidenreich 1, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33...

... cells such as macrophages and neutrophils that attack invading pathogens. Innate immune cells also process... to recognize a specific antigen, it finds and attacks pathogens or infected cells expressing that antigen... of Cell Culture External Resouces Immport : Bhattacharya S, Dunn P, Thomas CG, Smith B, Schaefer H, Chen...

...plasmids from this paper at Addgene. 12. Ronda, Carlotta, et al. (2016). "CRMAGE: CRISPR Optimized MAGE...

...specialties, theology, and the arts, and requiring the attainment of a bachelor's degree or its equivalent as a...

...s41587-019-0193-0 Walton, R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020). Unconstrained...

...to Tyler Jacks’ lab, this 3-day goal is is now attainable. The Jacks lab has recently introduced a new ...

...CATATG TTAATTAA GGCGCGCC CAATTG - 3' = 28 bp Bottom oligo: 3' - GTATAC AATTAATT CCGCGCGG GTTAAC - 5' =...AATTC CATATG TTAATTAA GGCGCGCC CAATTG G - 3' Bottom oligo: 3' - G GTATAC AATTAATT CCGCGCGG GTTAAC CAGCT - ...' - AATTCCATATGTTAATTAAGGCGCGCCCAATTGG - 3' Bottom oligo: 5' - TCGACCAATTGGGCGCGCCTTAATTAACATATGG - 3'...additional sites in tandem ( NdeI - CATATG , PacI - TTAATTAA , AscI - GGCGCGCC , MfeI - CAATTG ). The bottom...

...(AA)TGCCTACGTTAAGCTATAC, the oligos would be: Forward oligo: 5’ CCGG AATGCCTACGTTAAGCTATAC CTCGAG...CTCGAG GTATAGCTTAACGTAGGCATT TTTTTG 3’ Reverse oligo: 5’ AATTCAAAAA AATGCCTACGTTAAGCTATAC CTCGAG...CTCGAG GTATAGCTTAACGTAGGCATT 3’ Back to Top C. Cloning Oligos into pLKO.1 The pLKO.1-TRC cloning vector...

...magnesium, Corning 21-040-CV (cations can affect the attachment of adherent cells) 0.45 μm polyethersulfone filter...were transfected with the GFP-expression plasmid pRosetta using μg total DNA to μg PEI ratios of 1:1, 1:...