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Showing: 141 - 160 of 539 results
  1. Antibodies 101: Designing Your First Flow Panel

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    Blog Post
    ...bd-spectrum-viewer.   To make life easier, always try to use fluorophores with distant excitation/emission...bleeding, or spectral overlap, and why you should try to avoid or reduce it whenever possible. Finally,...Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Flow Compensation...When analyzing your cells using flow cytometry, you are typically measuring the presence or absence of...reporters, and/or dye stains used in your flow cytometry experiment are known as your "panel". In this ...from: https://emea.bd.com/panel-design/en/flow-cytometry-fluochrome-brightness-spillover Excitation...degree. The phenomenon of spectral overlap in flow cytometry is commonly referred to as bleeding. When two ...
  2. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post
    ...have mastered this technique so it might be worth a try, especially since the potential rewards could be ...continually being developed, so don’t be afraid to try novel, unpublished combinations of labeling strategies...reassignment–Expanding the chemistry in biology." Chemical Science 6.1 (2015): 50-69. Royal Society of Chemistry. 3. Ermolenko...distance between the donor/acceptor fluorophores (Stryer, 1978), a measurement that is unaffected by intervening...23667515. PubMed Central PMCID: PMC3647052. 11. Stryer, Lubert. "Fluorescence energy transfer as a spectroscopic...spectroscopic ruler."Annual review of biochemistry 47.1 (1978): 819-846. PubMed PMID: 354506. 12. Vilensky...
  3. Plasmid Cloning by PCR

    Type
    Blog Post
    ...your primer length and increase the Tm if you are trying to clone from genomic DNA, a cDNA library, or by...
Showing: 141 - 160 of 539 results