We narrowed to 971 results for: lars;
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TypeBlog Post...Moks 1987). These tags, however, tended to be very large and often required the incorporation of a cleavage...M, Enfors SO, Persson I, Nilsson B and Uhlen M. Large-scale affinity purification of human insulin-like...
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Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post...hormone therapy resulted in 76,000 fewer cases of cardiovascular disease, 126,000 fewer breast cancers, and ... Scientific Impact Are Evoloving Check Out Extracurricular Activities for a Strong Science Career Path... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog Post... science and tech get jobs. She is also a very popular speaker and workshop leader. Lauren Celano of Propel...Confident and Successful Industry Professionals”. This larger shop might appeal to some although most scientist... -
Rabies and Neuronal Tracing
TypeBlog Post...biological questions about the brain. To address this, molecular tools to map neuronal connectivity are widely ... Using these technologies in combination with molecular tools (e.g., optogenetic or chemogenetic receptors... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...existed. Recently, however, a particular tool has gained widespread popularity – and notoriety – as a tool... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...Synaptophysin-GFP. Synapse targeting GCaMP6s from the Rylan Larsen lab, now available in a ready to use AAV5 viral... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post...there’s no sense in spending valuable research dollars on a new secondary antibody if you don’t need it... better for probing poorly expressed targets. Similarly, rabbit derived antibodies may be the better choice... -
A Look at Addgene's QC Process
TypeBlog Post...The raw sequencing reads are assembled into a circularized plasmid sequence de novo (without the use of...pipeline. There are regions of plasmids that are particularly difficult to sequence and assemble using the... -
CRISPR Library Amplification
TypeProtocol... Pooled Libraries All CRISPR Pooled Libraries Molecular Biology Reference How do I process my Addgene ...libraries up to 200,000 individual plasmids. For larger libraries scale the number of cells and DNA accordingly... LB Agar + Antibiotic 245 mm bioassay plates (Molecular Devices, X602) Pro-Tip Pour these plates at least... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog Post...browser, Ensembl, and others. It builds on existing popular web services, currently Crispor and Crispresso,...below.) For guide design, we decided to adapt the popular guide design tool Crispor by Max Haeussler. With...