We narrowed to 1,073 results for: TOR;

...number of chemical and peptide-based proteosome inhibitors, glutathione S-transferase (GST), which can be...purification to get your protein as pure as possible? Vectors that offer different combinations of tags are readily...Protein (GFP) Fluorescent Protein Guide Expression Vectors from the Berkeley QB3 MacroLab Eric thanks his...

...applications? Fujii: We generated a series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral...joined Addgene's Advisory Board, as well as the Editorial Board of Scientific Reports and PLoS One. Additionally...especially for young researchers. It was a really nice incubator for me. In Japan, of course, it is optimized for...

...time-consuming, and complex to use outside of standard laboratories. In the case of the COVID-19 pandemic, qPCR ...each enzyme. In addition, Csm6, a CRISPR type-III effector nuclease, can be used in conjunction with Cas13...single-component programmable RNA-guided RNA-targeting CRISPR effector. Science 353:aaf5573 . https://doi.org/10.1126...

... (LshCas13a) to begin to characterize this new effector (Abudayyeh et al., 2016). In the endogenous CRISPR...fluorophore. The fluorescent signal can be used as an indicator to determine whether the target sequence is present...single-component programmable RNA-guided RNA-targeting CRISPR effector." Science 353(6299) (2016):aaf5573. PubMed PMID...

...on FRET techniques. By combining standard donor/acceptor FRET know-how with aptamers, aptabeacons (or aptatags...fluorophore protein. Years later, Samie Jaffrey’s laboratory took advantage of their deep knowledge of the...a higher melting temperature than its Spinach ancestors, and has a lower magnesium dependence. Though ...

...Western blot. When choosing beads, there are several factors to consider. Agarose beads are sponge-like and ...more stringent buffers may be needed. Since many factors, including salt concentration, ionic strength and...prevent this, include protease and phosphatase inhibitors in the lysis buffer, keep samples on ice, and...

...- e.g. codon choice may interrupt transcription factor binding sites) As you might imagine, it’s not ...not easy for humans to balance all of these factors on their own. Luckily, many researchers have created...Kimberly A., et al. "Selective charging of tRNA isoacceptors induced by amino‐acid starvation." EMBO reports...

... delivered as a pooled library via a lentiviral vector to cell lines that either stably expressed Cas9... screen did fail to identify some known ricin-regulators, but most of these genes are also essential for...hypersensitivity, or are in an enhancer, transcription factor binding site, or a coding region. Targeting these...

...logistical, like briefly explaining a gap in your job history or sharing relocation plans. They can be skills-based...in the possibility of joining your team as a postdoctoral researcher. Dear Hiring Manager, I am writing...your cover letter, and if possible, send it to a mentor or colleague for a second look and helpful feedback...

...research scientists, faculty, students, private sector researchers, or pretty much anyone applying the...studies on reducing antibiotic prescriptions for respiratory tract infections. The video abstracts provide...is not hard either. Here are some resources: ImpactStory, a website that helps researchers go beyond citation...