... Although we're storing away all of our holiday decorations, our holiday spirit remains. We would like...

... the two, use a conversion calculator or chart like the G Force calculator (Link opens in a new window...Introduction A centrifuge is a common piece of laboratory equipment used to separate different components...tabletop microcentrifuge with the lid open, the rotor with microfuge tubes in place, and the control panel...centrifuge, with tubes evenly distributed around the rotor. How to Centrifuge Now that you know the different...working smoothly. Place your sample tubes into the rotor so that they are distributed evenly around the center...sample. Place the centrifuge’s internal lid on the rotor (if it has one) and then close the external lid....

...Addgene Videos Page 5 Science Rap Videos and Their Creator: Tom McFadden Using Video to Share Your Science...

... particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (...

...by email.  About the author Niko McCarty is the editor of Asimov Press and is designing a genetic engineering...

...polymerase pLKO.1 - TRC Cloning Vector Cloning protocols for using the pLKO.1 vector, a backbone used by the ...addgene.org with any questions. Intro to the Lab Bench Introductory techniques designed to help you get started ...and Two (BSL-1 and BSL-2) Safety measures for laboratories operating at BSL-1 and BSL-2 Watch the Video...Video! Pipetting Learn about selecting the correct pipettor and pipette tip, how to dispense liquids, and ... its use Weighing Reagents Learn how to weigh laboratory materials on a balance Basic Molecular Biology...Watch the Video! Creating Bacterial Glycerol Stocks Store bacterial strains or plasmids for long term use ...

...Assay for Lentivirus You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral...marker. Determining the titer of your lentiviral vector allows you to control the multiplicity of infection...1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Inverted...heat-inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C. Pro-Tip Different brands and lots of FBS...protocol was developed using low titer lentiviral vectors (10 5 TU/mL). If you anticipate that your viral...units per mL (TU/mL) using either the dilution factors (method 1) or the volume of virus (method 2): Method...Fraction of cells with fluorescence D = Dilution Factor V T = Transduction Volume, mL For example: If 150,000...

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...Contribute your AAV data Just like Addgene’s plasmid repository, the AAV Data Hub is a community-driven project...

...filter. Aliquot 50–100 µL and store at -20 °C. Aliquots can be thawed and stored at 4 °C prior to use. Thawed...into sterile tubes. Store the tubes at -80 °C. After thawing the solution can be stored at 4 °C for up to...General Transfection You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral...1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents...heat-inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C. Pro-Tip Different brands and lots of FBS...