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We narrowed to 971 results for: lars;

Showing: 801 - 810 of 971 results

  1. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection... AAV1. Synapse targeting GCaMP6s from the Rylan Larsen lab are now available in a ready-to-use AAV5 viral...

  2. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created...xCas9 3.7. Base editor variants with xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7...

  3. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ... as the central nervous system (CNS). AAVs are popular tools because: 1) their genomes are easy to manipulate...difficult it is to trace individual cell’s axons, particularly the two green neurons near the arrows. When ...

  5. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...droplet separation (Witte et al., 2016). This is particularly important if your amplicon is longer than 150...ease of use, droplet digital PCR is becoming a popular option for PCR experiments.  References Furuta-Hanawa...

  6. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...RNPs are capable of cleaving genomic targets with similar efficiency as compared to plasmid-based expression... homology directed repair (HDR), and generating large genomic deletions. What are the advantages of Cas9...

  8. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency but no significant off-target...characterization of diverse class 2 CRISPR-Cas systems." Molecular cell 60.3 (2015): 385-397. PubMed PMID: 26593719...

  9. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...   Indirect ELISAs The indirect ELISA process is similar to that of a direct ELISA, but the indirect method... drawbacks of the competitive ELISA method are similar to those of the direct method. Namely, since the...
Showing: 801 - 810 of 971 results