Skip to main content

We narrowed to 1,073 results for: TOR;

Showing: 811 - 820 of 1073 results
  1. Five Popular Model Organisms

    Type
    Blog Post
    ... al., 2009). By injecting mice with retroviral vectors that harbored different mutations found in leukemia...Gerald Rubin’s lab who created a set of modular vectors that can be used to optimally control gene expression...discovery of RNAi in the worm actually rewarded depositors Andrew Fire and Craig Mello a Nobel Prize in ...
  2. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...either by electroporation of plasmids, or using AAV vectors. The Imai lab also developed a version of Tetbow...Livet, Jean, et al. "Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous...Markus Grompe. "Clonal tracing of Sox9+ liver progenitors in mouse oval cell injury." Hepatology 60.1 (...
  3. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... the microflora that reside there. Biotin is a cofactor that in its active form is covalently attached...consists of a biotin molecule attached to a photoreactive arylazide group. Under strong visible light,...single-step purification of tagged transcription factors in mammalian cells and transgenic mice.” PNAS 100.13...
  4. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...CRISPR, RNAi methods are often quicker and the repositories of validated small RNAs are vast and difficult...processing Following delivery into cells using viral vectors or plasmids, shRNAs hijack the endogenous miRNA...cloning strategies for constructing shRNA expression vectors. BMC Biotechnology, 6(1). https://doi.org/10.1186...
  5. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...too many activities too close to one another. Monitoring task progress. For this, I always make sure I... lists. Because I know what my goals are (see monitoring task progress), and I know that I’ve blocked ... give it a final read Browse list of previous depositors from Boston University Contact Dr. Gilmore directly...
  6. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...introduce or remove restriction sites), in mapping of regulatory elements (to mutate promoters/enhancers in reporter... addition to the “A” site already in the parent vector). Also shown are the “B” restriction sites, which...Browse Our Molecular Biology Protocols Find Empty Vectors for Your Next Cloning Experiment ...
  7. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 ...Guttman A, Thompson L. Vaccine Plasmid Topology Monitoring by Capillary Gel Electrophoresis. Curr Mol Med... JA, Carnes AE, Hodgson CP. Plasmid DNA vaccine vector design: impact on efficacy, safety and upstream...
  8. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...Aydin S (2015) A short history, principles, and types of ELISA, and our laboratory experience with peptide...knockout line for validation, please view this tutorial from Stuart Orkin's and Daniel Bauer's labs.  ...
  9. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...modular toolkits build single plasmid targeting vectors that encode the gRNA transcript and the repair ...recognized by the 2020 Nobel Prize in Chemistry), and Victor Ambros and Gary Ruvkun for their seminal work on....114.170423 Schwartz ML, Jorgensen EM (2018) SapTrap vectors for introducing point mutations with unc-119+ selection...
Showing: 811 - 820 of 1073 results