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  1. Colony Formation Titering Assay

    Type
    Protocol
    ...Assay for Lentivirus You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral...1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents...heat inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C. Pro-Tip Different brands and lots of FBS...Swirl gently to mix and place the dish in the incubator. Incubate for 48–72 h. Pro-Tip Different cells...colonies/total volume in the well (mL) x dilution factor e.g., If the 1:100,000 well has 75 colonies, then...the indicated serial dilutions of the lentiviral vector pRosetta . The following day, cells were treated...the indicated serial dilutions of the lentiviral vector pHAGE EF1α dCas9-KRAB . The following day, cells...
  2. Summer Fun at Addgene!

    Type
    Blog Post
    ...Landscape for Engineered Systemic AAVs” with the director of CalTech’s CLOVER lab, Tim Miles, PhD, we invite...
  3. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...tradition in which we choose our teams and start brainstorming months in advance. Our teams comb the news (...
  4. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...Functional data, if the report describes a functional vector such as a channelrhodopsin Images showing the injection...
Showing: 811 - 820 of 1074 results