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...The raw sequencing reads are assembled into a circularized plasmid sequence de novo (without the use of...pipeline. There are regions of plasmids that are particularly difficult to sequence and assemble using the...

...there’s no sense in spending valuable research dollars on a new secondary antibody if you don’t need it... better for probing poorly expressed targets. Similarly, rabbit derived antibodies may be the better choice...

... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...Synaptophysin-GFP. Synapse targeting GCaMP6s from the Rylan Larsen lab, now available in a ready to use AAV5 viral...

...Resources Molecular Biology Reference Molecular Biology Reference Jump To: Origins of Molecular Genetics...Learn about the basics of molecular biology, including molecular genetics, plasmids, sequencing, and ...and a collection of other molecular biology resources, including: Molecular Cloning Techniques Promoters...DNA molecule is known as DNA or molecular cloning. The advent of molecular cloning enabled scientists to...with Plasmids Plasmid Elements Types of Plasmids Molecular Cloning Common E. coli Strains Common Antibiotics...Plasmids are one of the most important tools in molecular biology. They allow scientists to isolate specific...have led to the understanding of many important molecular pathways and diseases. Here we present an introduction...

...Educational Resources Molecular Biology Reference Molecular Cloning Techniques Molecular Cloning Techniques...endonuclease) molecular cloning is the "classic" cloning method, and remains one of the most popular today. ...Learn about different molecular cloning techniques, focusing on seven common cloning methods. Educational...Cloning Yeast-Mediated & Oligo Stitching Resources Molecular cloning, or the creation of recombinant DNA, is...used in scientific research and discovery. With molecular cloning, scientists can amplify and manipulate...project . This guide highlights several of the most popular cloning methods for the creation of recombinant...for DNA cloning and expression contain several popular restriction enzyme sites, usually within the multiple...

... Molecular Biology Reference Molecular Cloning Techniques MoClo Guide Modular Cloning (MoClo...Plasmid Kits Blog: Plasmids 101 Modular Cloning Blog: Plasmids 101 Modular Cloning Applications and Kits...compatible with PhytoBrick parts. MoBiFC - Modular Bimolecular Fluorescence Complementation Toolkit Plant...enabling larger multi-gene constructs, multiplexed CRISPR editing, and more. Fungal Toolkit for Modular Cloning...Learn about modular cloning (MoClo) and find MoClo kits deposited with Addgene, including the MoClo Toolkit...Golden Gate Cloning Synthetic Biology Plasmids Modular Cloning (or MoClo) is a system for efficiently ...engineering, including CRISPR/Cas9 components. MoChlo: Modular Cloning Chloroplast Toolbox Plant Expression Scott...

...system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created...xCas9 3.7. Base editor variants with xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7...

...browser, Ensembl, and others. It builds on existing popular web services, currently Crispor and Crispresso,...below.) For guide design, we decided to adapt the popular guide design tool Crispor by Max Haeussler. With...

... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection... AAV1. Synapse targeting GCaMP6s from the Rylan Larsen lab are now available in a ready-to-use AAV5 viral...

...RNPs are capable of cleaving genomic targets with similar efficiency as compared to plasmid-based expression... homology directed repair (HDR), and generating large genomic deletions. What are the advantages of Cas9...