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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 971 results for: lars;

Showing: 821 - 830 of 971 results

  1. Ligation Independent Cloning

    Type
    Protocol
    ... like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Overview Ligation Independent...Transform Mix your treated vector and insert at a molar ratio of 1:2 or 1:3, using between 20 and 50 ng ...

  2. Video Library

    Type
    Protocol
    ...success in numerous fields such as microbiology, molecular biology or cell biology. Learn some of the key...samples. This is a commonly used technique for molecular cloning, such as PCR- or restriction enzyme-based...

  3. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ... a Liquid Bacterial Culture Introduction Many molecular biology techniques require highly purified and... of bacteria to grow (low copy plasmids require larger cultures). Centrifuge the culture to pellet the...

  5. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...from academic viral vector cores. Our titers were similar to those reported by these institutions. This protocol...Concentration of Addgene plasmid #59462 : 1.07 μg/μL Molecular Weight: 6208 bp x 650 dalton/bp or (g/mole)/bp...

  6. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...and reagents from other vendors should produce similar outcomes when using these protocols. However, please...plate washer (optional) Scale Microsoft Excel or similar software Reagents Purified antigen Purified recombinant...

  7. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...empirically, and that the cell population be monitored regularly for transgene expression. This protocol has been...over-confluent, trypsinize the cells and expand them to larger culture dishes, being sure not to cross contaminate...

  8. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...37°C, 5% CO 2 overnight. Although cells should regularly be passaged in DMEM + 10% FBS with penicillin/...limited to the implied warranties of fitness for a particular purpose, non-infringement, typicality, safety...

  9. CRISPR Guide Update

    Type
    Blog Post
    ...and additions describing CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3...

  10. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ... to me: Selective Endogenous eNcapsidation for cellular Delivery (SEND). On the fourth day of CRISPR, ...
Showing: 821 - 830 of 971 results