...amplification protocols that have been developed by the depositor for a specific pooled library available at Addgene...Addgene. We recommend following the depositor's specific protocol if one is available. If a pooled library...require modifications dictated by the originating laboratory for optimal results. If you obtained the pooled...amplification protocol is expected to obtain satisfactory results for libraries up to 200,000 individual...limited. Equipment Table top centrifuge BioRad Electroporator (MicroPulser TM , Bio-Rad 1652100) Reagents...time for a total of eight electroporations): Electroporator Conditions: Bio-Rad Micropulser Ec1 0.1 cm ...