We narrowed to 997 results for: lars;

... a Liquid Bacterial Culture Introduction Many molecular biology techniques require highly purified and... of bacteria to grow (low copy plasmids require larger cultures). Centrifuge the culture to pellet the...

...will need to use a phosphatase to prevent re-circularization of the recipient plasmid. You should treat ...Additionally, if your final product is going to be very large (>10kb) you might want to use electro-competent ...

...from academic viral vector cores. Our titers were similar to those reported by these institutions. This protocol...Concentration of Addgene plasmid #59462 : 1.07 μg/μL Molecular Weight: 6208 bp x 650 dalton/bp or (g/mole)/bp...

...and reagents from other vendors should produce similar outcomes when using these protocols. However, please...plate washer (optional) Scale Microsoft Excel or similar software Reagents Purified antigen Purified recombinant...

...empirically, and that the cell population be monitored regularly for transgene expression. This protocol has been...over-confluent, trypsinize the cells and expand them to larger culture dishes, being sure not to cross contaminate...

...37°C, 5% CO 2 overnight. Although cells should regularly be passaged in DMEM + 10% FBS with penicillin/...limited to the implied warranties of fitness for a particular purpose, non-infringement, typicality, safety...

...and additions describing CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3...

...Enhanced prime editing systems by manipulating cellular determinants of editing outcomes🎵 On the 5th ...

... to me: Selective Endogenous eNcapsidation for cellular Delivery (SEND). On the fourth day of CRISPR, ...

... technologies are going? What is the next hot molecular biology technology? Have you deposited your plasmids...