...independently of any other accessory proteins or co-factors, thus allowing for broad applications in a variety...

...Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids Recovering Plasmid DNA from Bacterial...temperature. Now screw on the top of the bottle and store the LB at room temperature. When ready to grow your...bacterial culture at 37°C for 12-18 hr in a shaking incubator. Note: Some plasmids or strains require growth... overnight incubation. Optional: For long term storage of the bacteria, you can proceed with Creating ...

... Protocols Video protocol guides for standard laboratory procedures. How-To Videos Screencapture walk-...enzymes Diagnostic Restriction Digest Protocol Storing and Handling Addgene Plasmids What to do after ...receive your plasmids from Addgene Instructions for Storing and Handling Plasmids Genomic Deletions with CRISPR...Electrophoresis Gel electrophoresis is the standard laboratory procedure for separating DNA by size for visualization...

...specific, individual proteins under endogenous regulatory control. But even with the advent of CRISPR/Cas9...

...applied antibodies and need to dry the membrane for storage, but plan to reprobe it later, strip it thoroughly...

...the appropriate primers to detect your targeting vector or gene of interest. 2. Breed your parental mice...

...quickly. Just make sure to incorporate the dilution factor in your calculation from OD to protein concentration...

...high-quality antibodies for IHC. Working with collaborators and support from the Brain Research Through ...

...Mol Syst Biol. 2014 Galit Lahav EYFP Gateway vectors with EYFP split at 175 Arabidopsis homolog of the...

...impacts in the way we study and treat complex or multifactorial diseases, enabling faster diagnosis and treatment...