We narrowed to 911 results for: TRO

...Design a Primer Diagnostic Restriction Digest Introduction Sequence verification of important plasmid features... do not sequence the entire plasmid. Addgene strongly recommends that you sequence any portion of the...What primers were used by Addgene during quality control? Addgene lists the primers used to obtain each ...

...cloning scars"). A “stuffer” sequence allows for electrophoretic separation of linearized vector from the reaction...linearized vector from the reaction mixture by gel electrophoresis followed by gel purification . The cut vector... your chances of success. Set up a vector only control with water instead of the insert. Incubate the ...

...Protocols Centrifugation Centrifugation Protocol Intro to the Lab Bench Check out more protocols and videos...videos to help you get started in the lab! Introduction A centrifuge is a common piece of laboratory equipment...the rotor with microfuge tubes in place, and the control panel where you can set the time and speed needed...

...and 30 bp annealing to the target sequence. Avoid strong secondary structures in the homology region. Hairpins...seams between the assembled parts. Tips and Troubleshooting “Stitching” fragments together using oligos...oligos. This technique is especially useful for introducing promoters, terminators, and other short sequences...

... Biosafety Levels One and Two (BSL-1 and BSL-2) Intro to the Lab Bench Check out more protocols and videos...videos to help you get started in the lab! Introduction There are four biosafety levels. This protocol...protocols require bloodborne pathogens training. It is strongly recommended that anyone participating in BSL-2...

...like... Viral Vector Guides Mol Bio Protocols Introduction This protocol can be used to produce lentivirus...channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container pH meter Stir plate... should be aliquoted and snap frozen in liquid nitrogen and stored at -80 °C as soon as possible to avoid...

...like... Bacterial Transformation Agarose Gel Electrophoresis Agarose Gel DNA Purification Streaking and ...Bacteria Inoculating a Liquid Bacterial Culture Introduction Many molecular biology techniques require highly...window) Qiagen , (Link opens in a new window) Invitrogen , and (Link opens in a new window) Promega sell...

...standard Polymerase Chain Reaction (PCR) is an in vitro method that allows a single, short region of a DNA...product can be verified based on size by gel electrophoresis . Protocol Video Watch the protocol video below...to each reaction. The ideal setup for this troubleshooting step is to do one reaction with each, and one...

...Vector Guides Virus Blog Posts Mol Bio Protocols Introduction The AAV Titration protocol can be used to determine...samples being tested. Always include a No Template Control (NTC), i.e master mix + water Whenever possible...duplicate. Do not forget to include a no template control ( NTC = master mix + water ). Add 15 μL of Master...

... Versus Gamma-Retrovirus Lentiviruses and gamma-retroviruses fall under the Retroviridae family. The genome...Glossary Lentiviruses are a type of retrovirus and belong to the Retroviridae family. Infection with lentiviruses...variety of cell types than retroviruses. Both lentiviruses and gamma-retroviruses use the same packaging ...packaged by gamma-retroviral packaging systems, and vice versa. For more about gamma-retroviruses, see Addgene's...Panch, S. R., Jin, P., & Stroncek, D. F. (2022). Genome-wide profiling of retroviral DNA integration and its..., leading to disease. Lentivirus A retrovirus from the Retroviridae family. Characterized by long incubation...BIV), and feline immunodeficiency virus (FIV). Retroviruses are partially characterized by their ability...