We narrowed to 113 results for: PIP

...single channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette 2–50 µL multichannel...diluent, pipette 10 times, to remove virus from pipette tip. To mix the dilution, set the P200 pipette to 200...multichannel pipette 20–200 µL multichannel pipette Ice bucket 96-well freezer blocks Vortex, VWR, 10153-... 200 µL and pipette 10–20 times. Use a new pipette tip to transfer 200 µL of the first dilution into the...mixes and samples on ice before use. Wipe down all pipettes and surfaces with 10% bleach. Safety Warnings ...well before seeding. Mix each well with a 1 mL pipette 5–10 times. The final volume in the well is 1.5...6-well plates. Be sure to use a new aspirating pipette for each well as to not contaminate cell conditions...

...channel pipette 20–200 µL multichannel pipette Multichannel pipette reagent reservoirs Pipette controller...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...Neptune 3745.X Pipette tips, 1000 µL, VWR 76322-154 Pipette tips, 10 µL, VWR 76322-132 Pipette tips, 200 µL... µL, VWR 76322-150 Pipette tips, 20 µL, VWR 76322-134 Pipettes, 10 mL, VWR 89130-898 1 L polystyrene bottle...microfuge tube and vortex. 0 ng/µL : 450 µL PBS only. Pipette 50 µL into the first three wells of each row, using...wash buffer (0.05% Tween-20 in PBS) as follows: Pipette 0.5 mL of Tween-20 to into 999.5 mL 1X PBS Cap ...aside. Aspirate the wells and use a multichannel pipette to add 200 µL wash buffer to each well. Washing...

...Heating plate Pipettors 1–10 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...single channel pipette 1–10 µL multichannel pipette 2–50 µL multichannel pipette 20–200 µL multichannel...multichannel pipet to minimize pipetting error and variability Mix samples very well by pipetting back and...multichannel pipette Reagents Universal SYBR Master Mix 2X Primer pair targeting AAV2 ITR ( Aurnhammer et al., ...water Microcentrifuge tubes 96-well optical plate Pipette tips General Considerations Always run standards...that result from a variety of causes, such as pipetting error and sample evaporation. Make the master ...before use. Use a reservoir and a multichannel pipette to dispense the master mix into the wells. Reagent...

...channel pipette Reagents Aspirating pipette, VWR 414004-265 5 mL pipette, VWR 89130-896 10 mL pipette, VWR...single channel pipette 0.5–10 µL single channel pipette 20–200 µL single channel pipette 2–20 µL single...VWR 89130-898 25 mL pipette, VWR 89130-900 50 mL pipette, VWR 89130-902 50 mL conical tubes, VWR 89039-...A2 Biological Safety Cabinet 4 °C Refrigerator Pipette controller Benchtop centrifuge compatible with ...tubes, VWR 76289-498 Before Starting Wipe down all pipettes and equipment with 10% bleach prior to use. Warm...the remaining space in the column with PBS and pipette several times to mix. Centrifuge at 3100 x g for...

...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...Invitrogen 26619 Pipette tips, 10 µL, VWR 76322-132 Pipette tips, 20 µL, VWR 76322-134 Pipette tips, 200 µL...VWR 76322-150 Pipette tips, 1000 µL, VWR 76322-154 Pipettes, 5 mL, VWR 89130-896 Pipettes, 10 mL, VWR 89130...single channel pipette Pipette controller Microcentrifuge Electrophoresis chamber Power supply Rocking ... 89130-898 Pipettes, 25 mL, VWR 89130-900 Pipettes, 50 mL, VWR 89130-902 Gel loading tips, Corning 4853...

...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...single channel pipette Hemocytometer or cell counter Ice bucket CO 2 incubator Pipet controller Hazardous...Neutralize trypsin by adding 10 mL of DMEM Complete. Pipet back and forth vigorously multiple times to obtain...with the cells harvested in the previous step. Pipette back and forth or shake vigorously to obtain a ...total of 5 mL of cell lysis buffer (recipe above). Pipet back and forth to resuspend each pellet completely...adding a total of 12.5 mL of cell lysis buffer. Pipet back and forth to resuspend each pellet completely...

...Fix and label cells Equipment Pipette controller Pipette tips and pipettes Rocking platform Tweezers Fluorescent...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...single channel pipette Reagents 1X PBS Microcentrifuge tubes Sterile Poly-D-lysine coated coverslips HeLa...

...single channel pipette Pipette controller Pipette tips and pipettes Spectrophotometer Heat block Mini gel...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...

...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Inverted fluorescence...cells into 14 mL of DMEM complete. Mix well by pipetting or inverting. Aliquot 2 mL of cell suspension ...

...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container pH meter Stir...out of the previously seeded 10 cm plate. Slowly pipette the transfection mix onto the 10 cm plate, being...