We narrowed to 532 results for: abo.3

...." F1000Research 3 (2014). Pubmed PMID: 25653845. Pubmed Central PMCID: PMC4304227. 3. Sauermann, Henry...positions within 5-7 years of earning their PhDs (3), showing that the ‘standard’ pathway into academia..., Eve. "Looking out for future scientists." eLife 3 (2014): e04901. Pubmed PMID: 25291257. Pubmed Central... the Jackson Laboratory for Genomic Medicine and a Senior Research Associate at the Labor and Worklife...increasing data collection about the scientific workforce, in particular about postdocs. Among other involvements...Research Symposium Boston 2015 – Academic Data and the Labor Market. For more information on the symposium as...generating too many trainees per PI” “Lack of awareness about how the system operates and functions” “Complete...

...plasmids ready for deep mutational scanning.     3 reasons to be interested in deep mutational scanning... Taq polymerase, 2) increased PCR extension time, 3) increased concentration of MgCl2, 4) increased concentration...synthesize a complementary mutant strand (step 2 and 3). Then the uracil-labeled template strand is degraded ...mutagenesis method are 1) ease of use, 2) time savings, and 3) ability to perform single site or multi-site saturation...Mutagenesis Protocols, pg. 103-109. PubMed PMID: 20676978. 3. Wrenbeck, Emily E., Justin R. Klesmith, James A. ... all desired codon changes (step 1 in the image above). A second universal primer is used to synthesize...first followed by the top strand. Both produced about the same number of positive clones. See Supplementary...

...antigen. J Gen Virol., 73 (Pt 3), 653–660. https://doi.org/10.1099/0022-1317-73-3-653. PMID 1372038. Randall...comparable to GFP during early embryogenesis (Figure 3). mScarlet-I3 also offered clearer signals due to ...autofluorescent tissues like the intestine. Figure 3: Expression of fluorophore-tagged GOLG-4 through eight...disruption in Mycobacterium abscessus. J Bacteriol., 206(3), e0033523. https://doi.org/10.1128/jb.00335-23.  ...more feasible, the lab of Alessio Ciulli and collaborators developed CRBNmidi (Kroupova et al., 2024). ...stepwise, achieving mutation efficiencies of up to about 9% of colonies (102 to 104 times higher than traditional...Bentley Time to get flexible! Alex Dunn’s lab and collaborators have deposited a new mechanosensor called PILATeS...

... step involves digesting phage genomic DNA into 1-3 kb (approximately gene-sized) fragments so that these...bulk of resultant fragments falling between 1 and 3 kb results in optimal likelihood of capturing a whole...filter paper and saturate each with lysis solution (~3 ml each), pouring off any excess. When your protein...Expression Vectors in the FX Cloning System Kit  Step 3: Probe random phage DNA fragments with live host cells...PMID: 27536293. PubMed Central PMCID: PMC4971087. 3. Simpson, David J., Jessica C. Sacher, and Christine..." Chemical Reviews (2016). PubMed PMID: 27319741. 3. Simpson, David J., Jessica C. Sacher, and Christine...our planet, but we still know collectively little about how they infect and influence the evolution of their...

...crRNACpf1 Endonuclease size spCas9: ~4 kbsaCas9: ~3 kb ~3.9kb crRNA/gRNA length gRNA: ~100 nt crNA:... Lastly, an AAV vector was used to multiplex edit 3-4 genes in a primary culture of mouse neurons and ...and GFP.  Of these neurons, ~15% had indels at all 3 targeted loci.  See the chart below for a summary ...Endonuclease of a Class 2 CRISPR-Cas System. Cell,163(3), 759-771. PubMed PMID: 26422227. PubMed Central PMCID...targets compared to Cas9.  Read on to learn more about Cpf1 multiplexing.  For an in-depth review of Cpf1... Addgene. If you have any questions or thoughts about Cpf1 multiplexing, leave them in the comments below...

...Recombinant DNA by CcdB.” Biotechniques. 1996 Aug;21(2):320-3. PubMed PMID: 8862819. 2. Bahassi, EM., et al. “F ...Microbiol. 1995 Mar;15(6):1031-7. PubMed PMID: 7623659. 3. Bernard, P., et al. “Positive-selection vectors using...DNA cleavage by gyrase.” J Mol Biol. 1993 Dec 5;234(3):534-41. PubMed PMID: 8254658. 5. Bernard, P. and ...DNA-topoisomerase II complexes.” J Mol Biol. 1992 Aug 5;226(3):735-45. PubMed PMID: 1324324. 6. Tam, JE. and Kline...in modern cloning, and why should you learn more about it? Read on to find out how ccdB can make your cloning...of this system for enhancing cloning efficiency about 20 years ago and developed cloning vectors to harness...

...Scientific reports 3 (2013). PubMed PMID: 24284873. PubMed Central PMCID: PMC3842082. 3. Mashiko, Daisuke...pCAG-EGxxFP target plasmid contains overlapping 5′ and 3′ EGFP fragments under the ubiquitous CAG promoter....classified into four groups (4; brighter than control, 3; same as control (Cetn1/sgRNA1), 2; darker than control...sequence the resulting plasmid. It can take roughly 3 months from the day you decide to do a gene modification...the pX330 plasmids used in the validation assay (above) and it is just as effective (Mashiko et al., 2014...Engineering with CRISPR Validate Your Genome Edit Learn about Non Homologous End Joining and Homology Directed...

...Topic Page Learn about Non-Homologous End Joining Learn about Base Editing Read 3 Tips to Improve ... break is resected by nucleases to create a ssDNA 3’ overhang. This will serve as both a substrate for... repair (DSBR) In the classical DSBR pathway, the 3’ ends invade an intact homologous template to serve...the non-invading strand at the other DSB end. The 3’ end of the non-invasive strand is elongated and ligated... donor DNA. Richardson CD, et al. Nat Biotech. 34(3); (2016). PubMed. Efficient homology-directed repair...

...the CEO of 3-D Molecular Designs.     Additional Resources on the Addgene Blog Learn All about CRISPR Use...MSOE center for BioMolecular Modeling and the CEO of 3-D Molecular Designs. Have you ever held your favorite...models were useful in that they not only traced the 3-D path of the protein’s backbone in three-dimensional...arginine 53 really is right there, at the end of helix 3…just like we said it was”.   Seeing is believing. ...Until then, you can only wonder what I am talking about. How do researchers use physical models of protein...students work closely with local researchers to learn about their research while creating physical models of...model is an effective teaching tool. You can read about Jacob’s initial reaction to this model on his blog...

...field, (2) that you have three 3 years of research experience in the field, (3) that you have an offer of ...applications and approved 91,023 during the first 3 quarters of 2017. As an international researcher and...well positioned to advance the proposed endeavor; (3) The foreign national’s proposed endeavor will be ...for this category. To qualify, you need to satisfy 3 out of the 10 criteria listed below. An international...of the institute. For the EB-1B, you need to prove 3 threshold requirements: (1) that you have international... you must show that your company employs at least 3 full-time people engaged in researcher activities.... data and the list goes on and on. As you think about your career (and your future in general), keep in...