We narrowed to 641 results for: ato

...either the optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato under the control of...Here's what you'll find in this post: Glutamate indicators with increased sensitivity and tailored deactivation...viral preps targeting interneurons Glutamate indicators with increased sensitivity and tailored deactivation...imaging rapid synaptic events such as in the somatosensory cortex, while iGluSnFR4s produces larger signal-to-noise..., Iyer, R., … Podgorski, K. (2025). Glutamate indicators with increased sensitivity and tailored deactivation...Glypicans By Ashley Waldron Glypicans are important modulators of cell signaling pathways during embryonic development...Shilatifard Lab was able to identify both new and known regulators at the FOS gene promoter and the MYC locus (Figure...

...Suntag” Cas9 activator that recruits up to twenty copies of the p65-HSF1 transcriptional activator to each ...and engineered combinations of transcriptional activators to induce gene transcription at genomic sites... vitro settings due to the large size of Cas9 activators, which largely precludes delivery by adeno-associated...will fit). Cre-dependent expression of the SPH activator paired with AAV sgRNA delivery make the SPH mouse...calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin-1 (pan-neuronal) promoters...Neuron-optimized viral vectors Alternatively, Cas9 activators can be delivered to the CNS by lentivirus, which...expression of dCas9-VPR (Figure 2), a popular Cas9 activator developed by the Church Lab. Neuron-specific expression...

...editing assemblies are sensor-actuators, either transcriptional regulators or chromatin-modifying enzymes...s chromatin status into account (Fig 1a). The laboratory of Karmella Haynes at Emory recently contributed...with examples of potential synthetic chromatin mediators and probes. Image from Priode et al., 2021. ...flexibility of the single-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying...interactions By Jennifer Tsang GFP-based Touching Nexus (G-baToN) is a new tool developed by Rui Tang in Monte Winslow...label cells after cell-cell interactions. Using G-baToN, “sender” cells express GFP on the surface and “...receiver cell. Image from Tang et al., 2020.   G-baToN can also be applied to other fluorophore/antibody...

...single-molecule biophysics Fast and responsive voltage indicator ONE-GO biosensors kit Antibodies for neurodegeneration...lab recently "broke the PE efficiency barrier in tomatoes" by combining several approaches (Vu et al., 2024...chaperone, RT: reverse transcriptase. Right: T1 tomato plants that inherited an edited gene to confer ...the team was able to drive prime editing in both tomatoes and Arabidopsis, suggesting the improvements may... species. In addition, they generated offspring tomato plants that inherited the genomic edit but not ...prime editing enables heritable desired edits in tomato and Arabidopsis. Nature Plants, 10(10), 1502–1513...2024.12.20.629623.  A fast and responsive voltage indicator with enhanced sensitivity for unitary synaptic...

...finally decide the LEGO laboratory was good enough. There are no pre-made LEGO laboratory sets so we had to ...to show people the very human struggles in the laboratory routine. We hope that sharing and laughing at...the LEGOs were mirrored by the nearly infinite regulatory capabilities of gene pathways just waiting to...was creating a LEGO stage that resembles a real laboratory. It only took one night to build the first model...use our imaginations to mold it all into a full laboratory. It is priceless to see our “toys” gaining life...working as scientists in a custom made, colorful laboratory. When we started, the hardest part of the process...building each scenario, I go “WOW, now I have my own laboratory” (the main character is much more than a piece...

...Cancer Institute! Sonic Hedgehog, an important regulator of embryonic development The largest conference...the hedgehog signalling pathway, which is a key regulator of all bilateral animal development. SHH plays...publication, Ivics et al 1997. Spaghetti Squash, a regulator of cellular contraction You may think that a gene...vessels). Spaghetti squash (Sqh) is the Drosophila Regulatory Light Chain (RMLC) of nonmuscle myosin II (Karess... Jordan and Karess, 1997), which contains the regulatory regions that are necessary for myosin activity...monster, a meiotic arrest gene required for spermatogenesis One of our smaller conference rooms is named... meiotic arrest gene that is necessary for spermatogenesis. Flies that lack cookie monster are sterile...

...doi: 10.1038/s41592-022-01597-x. New glutamate indicator iGluSnFR3 Improving on the glutamate sensor iGluSnFR...PDGFR.codonopt    Aggarwal A, et al. Glutamate indicators with improved activation kinetics and localization...have developed a new genetically-encoded voltage indicator (GEVI) called JEDI-2P (Liu et al. 2022) optimized...Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell. 2022 ...has generated a new genetically encoded calcium indicator using small monomeric NIR fluorescent proteins... of a Small Near-Infrared Fluorescent Calcium Indicator. Front Cell Dev Biol. 2022 Jun 29;10:880107. doi...they developed a family of fluorescent potassium indicators with a range of binding affinities (Torres Cabán...

...genes. We’ve created enough regulatory elements, CRISPRs, repressors, activators. We’ve been putting together...CRISPR/Cas genetic circuits are linked to the regulatory networks within E. coli host cells. ...in building genetic circuits is getting enough regulatory proteins that don’t interfere with each other...programmable that you can make a very large number of regulators. That basically means larger genetic circuits...that could conceivably be as large as natural regulatory networks. Addgene: What kinds of things could... pattern, shape. All of that is controlled by regulatory networks in the plant. If we want to access that...CRISPR is how much easier it is to work with than regulatory proteins. There are still things we haven’t solved...

...In-Fusion cloning molar ratio calculators Lab math calculators These calculators can help you with common...lab. Molarity calculator Promega's Biomath Calculators Primer resuspension calculator (sign-in required...Golden Gate Reactions Cloning ligation reaction calculators These links can help you calculate how much insert...assembly, or In-Fusion cloning.  NEB Ligation Calculator: This tool works for any cloning method. You ...required.)  DailyCalcs Science Calculator app for iPhone Reagent and Data Repositories These links can help...

...transcriptional regulator to modulate expression at that locus. The use of regulators allows a greater...shifted in recent years toward tinkering with regulatory networks and multiplex gene control. However,...approaches are often met with the challenges of regulatory bottlenecks and their scope is limited by the...scaffolds that can recruit various transcriptional regulators (Zalatan et al. 2015).  These scaffolds are stretches...levels dependent upon the type of transcriptional regulator or the number of designed scaffold binding sites...with distinct programs controlled by each dCas9 regulatory switch. By no means limited to the field of metabolic...scaffolds can recruit various transcriptional regulators to alter gene expression at the targeted site...