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We narrowed to 643 results for: ATO

Showing: 161 - 180 of 643 results

  1. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ... bank account, business cards, incorporation, laboratory lease, etc. I still flip to that page in my lab...business. We ended up applying to a business accelerator freely stating that we had an idea, knew how ...rough day, and we have great partners in this accelerator program who want us to succeed. We’re excited...duty to pursue a degree in a synthetic biology laboratory and received her M.S. from CSU in 2014. Outside...

  2. Common Injection Routes in Mice

    Type
    Blog Post
    ...common use of intradermal injections is studying inflammatory responses, as this method is useful for analyzing... correct location requires a lot of skill and anatomical knowledge. Intrathecal injections are used for...Routes of administration. In H. Hedrich (Ed.), The Laboratory Mouse. Academic Press. Turner, P. V., Brabb, ...M. A. (2011). Administration of substances to laboratory animals: routes of administration and factors...

  3. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...with Addgene by year. (C) Cumulative number of laboratories that have deposited CRISPR plasmids by year....nuclease into three functional groups: nucleases, modulators, and chimeric effectors. Nucleases generate a... a classic double-stranded break, while modulators regulate transcription and chimeric efforts make a ...deposited and distributed CRISPR plasmids. But modulators and chimeric effectors, made available in 2013...

  4. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...up with poor droplet quality. Using a droplet generator, the sample and the droplet generation oil are...Pipette slowly If you are using a manual droplet generator, you will have to be careful about transferring...transferring your droplets from the droplet generator to the PCR plate. Maintaining a high droplet count is important...sample. Even if you are using an automated droplet generator, you should avoid pipetting too quickly when making...

  5. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...m a father of three, a scientist and science communicator, and I strive to be a volunteer and contribute...scientist. When our elementary school needed a new coordinator for the annual Math & Science Night, I took the...contacting our school district’s science curriculum coordinator with minimal luck, but eventually the 8th grade...great feedback from teachers and other science communicators.  What can CRISPR do? I spent more time on...

  6. Tips for arabidopsis transformation

    Type
    Blog Post
    ... J. (2002) Arabidopsis : a laboratory manual. Cold Spring Harbor Laboratory Press. Available at: https...insertion likely precipitated domestication of sweet potatoes (Kyndt et al., 2015)! It sounds simple, and it.... et al. (2015) ‘The genome of cultivated sweet potato contains Agrobacterium T-DNAs with expressed genes...

  7. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...is the Laboratory Operations Manager at Addgene.  In addition to helping with daily laboratory operations...through barcoded tubes that are scanned into our Laboratory Information Management System (LIMS), a database...operations, she also works on improving laboratory efficiency through automation.       More Resource Avaliable...

  8. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...amount of grant funds available and the increasing regulatory process for grants has fostered the demand for...professional. Dr. Cook served as a principal investigator for several research grants. One of the benefits...benefits of being a principal investigator is that you are asked to review grant proposals from others. ...worked for a health department and then for the regulatory services department as our healthy homes program...

  9. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...time-consuming, and complex to use outside of standard laboratories. In the case of the COVID-19 pandemic, qPCR ...platforms to reliably detect nucleic acids at the atomolar level. The Zhang lab has adapted natural RNase...reporter enables SHERLOCK to detect DNA or RNA with atomolar sensitivity and single base pair mismatch specificity... of HPV, HPV16 and HPV18, from human cells, at atomolar levels, within one hour. DETECTR thus has the ...

  10. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...recently was invited to attend the first Principal Investigator meeting of the NSF EDGE (Enabling Discovery ...parenting strategies and make important ecological indicators since species live in water and on land. Current...that tastes yucky (and is poisonous) for bird predators - birds eat a caterpillar once, vomit, and never... expensive. They are using a cool fluorescent indicator system to rapidly identify recombinants. Arthropods...

  11. Hot Plasmids Spring 2024

    Type
    Blog Post
    ... vector preps expressing the Voltron2 voltage indicator, created by the Schreiter Lab and the GENIE Project... available! Building on their earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they...The same A-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar improvements in...fluorescent signal at baseline voltage), the investigators found that administering these AAVs with a dilute...

  12. The Challenges of Cell Culture

    Type
    Blog Post
    ...simple as a piece of paper on the front of the incubator containing the passage number, cell line, and ...book and on the actual flasks of cells in the incubator). This means any other scientist who wishes to...The LGC Group also provides standards and has laboratories to carry our independent quality control for...culture contamination." Archives of pathology & laboratory medicine 133.9 (2009): 1463-1467. PubMed PMID...

  13. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...characteristics that make them ideal for studying in a laboratory environment. We’ve covered five model organisms...have been specifically developed to thrive in laboratory settings. One of its most attractive features...characterized genome, and is easily maintained in a laboratory environment. What sets Arabidopsis apart from.... The Norway rat, from an obnoxious pest to a laboratory pet. ELife, 9. https://doi.org/10.7554/elife.50651...

  14. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ... 37°C heat block, LED blue light illuminator or UV light illuminator. Approach: Highly similar to the ...method based on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch...SARS-CoV-2 Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang...

  15. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...transcriptional repressor), and dCas9-VP64 (transcriptional activator)-containing plasmids. Each destination vector ...one. This approach is especially useful for combinatorial analyses. For example, if you were to develop...A versatile modular vector system for rapid combinatorial mammalian genetics.” Journal of Clinical Investigation...Cress, Brady F., et al. “CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR Arrays for dCas9...

  16. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...pAAV-Ef1a-fDIO-ChrimsonR-tdTomato AAV9 Controls Jensen New serotype pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA AAV9...multiple serotypes pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] AAV5, AAV8 Optogenetics Boyden New serotypes...

  17. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...ways scientists have adapted them for use in the laboratory. The discovery of GFP The story of how fluorescent...sequence. Soon after that, in 1994, Prasher’s collaborator Martin Chalfie expressed GFP in exogenous organisms...studying it grew. In particular, studies in the laboratory of UCSD biochemist Roger Tsien resulted in much...

  19. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ...transduction of Cas9-expressing (Rosa26-Cas9) hematopoietic stem cells with a lentiviral vector containing...LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in...G., & Sharpe, A. H. (2024). X-CHIME enables combinatorial, inducible, lineage-specific and sequential ...

  20. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...Vampire bacteria Vampirovibrio chlorellavorus is a predatory bacteria that attaches to the cell wall of green...forcing it out of hiding to become exposed to predators. Hungry birds spots the “caterpillar” and then... "Back from the dead; the curious tale of the predatory cyanobacterium Vampirovibrio chlorellavorus." ...
Showing: 161 - 180 of 643 results