Addgene

We narrowed to 294 results for: SPI;

Showing: 241 - 270 of 294 results

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Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Type

Blog Post

...physical size of the genomes is still ~4.7kb. But despite this, larger functional transgene products were...
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Type

Blog Post

...wheat, mushroom, and rice (Khatodia et al., 2016). Despite the almost universal functionality of the CRISPR...
Using Phosphoserine to Study Protein Phosphorylation

Type

Blog Post

...organisms lacked a classic cysteinyl tRNA synthetase, despite abundant incorporation of cysteine in their proteomes...
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Type

Blog Post

...strategy may be a better option than direct cleavage. Despite the potential difficulties in translating these...
Treating Muscular Dystrophy with CRISPR Gene Editing

Type

Blog Post

...wasting leading to death at around 30 years of age. Despite the amount of research conducted on DMD, there ...
Thoughts on the Future of Research 2015 Boston Symposium

Type

Blog Post

...clearly not equitable to that of other trainees despite the fact that the underrepresented individual is...
Viral Vectors 101: AAV Serotypes and Tissue Tropism

Type

Blog Post

...through our in-stock viral prep service. AAV2 Despite being isolated second, AAV2 is the best studied...
Plasmids 101: Repressible Promoters

Type

Blog Post

...to the promoter and turning transcription OFF. Despite their bacterial origins, Tet systems function well...
CRISPR 101: Epigenetics and Editing the Epigenome

Type

Blog Post

...methyltransferase from the prokaryote Mollicutes spiroplasma (M. Sss1) (termed MQ1.) The Q147L mutation improves...
Is this the right place for me? 8 tactics for choosing a lab

Type

Blog Post

...Will you learn how to run a lab if that is your aspiration? Will you help or at least observe grant writing...
Recombinase-based State Machines Enable Order-dependent Logic in vivo

Type

Blog Post

...described in Nielsen et al (3) and Lou et al (4). Despite choosing proper register parts and designs, we ...
Important Considerations When Using AAVs

Type

Blog Post

...to their Biosafety Level-2 or 2+. Please Note: Despite the fact that AAVs themselves are not pathogenic...
Quick Guide to All Things Lentivirus

Type

Blog Post

...order to aid viral binding. This method, called spinoculation, has been found very effective for T cells transduction...
When Fidelity Matters: A frank discussion about ligase fidelity

Type

Blog Post

...fidelity") better than T4 DNA Ligase (21, 25, 26). Despite this higher fidelity, Taq DNA Ligase can still ...
Mycoplasma Contamination: Where Does It Come From and How to Prevent It

Type

Blog Post

...these containers. Clean up any media or culture spilled immediately. Water baths, liquid nitrogen containers...
A History of Genome Engineering in Popular Culture

Type

Blog Post

...advancements and the noteworthy work of fiction that it inspired. CAUTION: Spoilers alert! Pre-DNA Era H.G. ...
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

Type

Blog Post

...Boyden et al., 2005; Deisseroth et al., 2006). Despite its relatively recent development, the history ...
9 tips for a successful postdoctoral experience

Type

Blog Post

...new pieces of data, your talk will probably not inspire you or the audience. Ask yourself: What is the...
Antibodies 101: Validation

Type

Blog Post

...to be present and recognizable by your antibody despite the manipulation. Additionally, not all sample ...
Antibodies 101: Designing Your First Flow Panel

Type

Blog Post

...panel-design/en/flow-cytometry-fluochrome-brightness-spillover Excitation Laser Color Very Bright Bright...
Cancer and the Immune System: Deciphering the Relationship

Type

Blog Post

... is to be successful, cancer immunotherapy must aspire to be a field of healthcare as dynamic as cancer...
With an Eye Towards the Future, We Look Back at the March for Science

Type

Blog Post

...was supremely proud of Nashville that morning. Despite the rain, about 4,000 supporters of science showed...
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

Type

Blog Post

...postdoctoral training from Massachusetts General Hospital and Harvard Medical School. Connect with her on...
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

Type

Blog Post

...quarters of 2017. As an international researcher and aspiring entrepreneur, you have many career options available...
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

Type

Blog Post

...party goers, the piñata bursts and its contents spill to the ground. There is something akin to a frenzy...
Immunology Research Plasmids and Resources

Type

Collection

...1 Gp95, NT3 SPP1 secreted phosphoprotein 1 BNSP, BSPI, ETA-1, MGC110940, OPN SST somatostatin SMST SSTR1...
15 Hot Plasmids from 2017

Type

Blog Post

...induction of structural plasticity in single dendritic spines. The set of CyRFP constructs available at Addgene...
Plan Your Experiment

Type

Guide

...containing a C nucleotide at the same position in spite of being a perfect match for the target sequence...
22 Hot Plasmid Technologies from 2014

Type

Blog Post

...for spatial proteomic mapping in living cells. Despite these advances, the Ting lab’s first-generation...
Molecular Biology Reference

Type

Guide

... selective pressure to retain the plasmid DNA, despite the added replication burden, as bacteria without...

Showing: 241 - 270 of 294 results

Results per page:

Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Using Phosphoserine to Study Protein Phosphorylation

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Treating Muscular Dystrophy with CRISPR Gene Editing

Thoughts on the Future of Research 2015 Boston Symposium

Viral Vectors 101: AAV Serotypes and Tissue Tropism

Plasmids 101: Repressible Promoters

CRISPR 101: Epigenetics and Editing the Epigenome

Is this the right place for me? 8 tactics for choosing a lab

Recombinase-based State Machines Enable Order-dependent Logic in vivo

Important Considerations When Using AAVs

Quick Guide to All Things Lentivirus

When Fidelity Matters: A frank discussion about ligase fidelity

Mycoplasma Contamination: Where Does It Come From and How to Prevent It

A History of Genome Engineering in Popular Culture

FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

9 tips for a successful postdoctoral experience

Antibodies 101: Validation

Antibodies 101: Designing Your First Flow Panel

Cancer and the Immune System: Deciphering the Relationship

With an Eye Towards the Future, We Look Back at the March for Science

Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

Immunology Research Plasmids and Resources

15 Hot Plasmids from 2017

Plan Your Experiment

22 Hot Plasmid Technologies from 2014

Molecular Biology Reference

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