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...We are excited about our new partnership with Allele Biotechnology which allows researchers to deposit...blue-shifted compared to its parent protein CFP484. Collaborating with scientists from Florida State University...mWasabi plasmids here! One of the most notable things about these proteins is that they are monomeric fluorescent... fluorescent protein blog posts Find blog posts about choosing fluorescent proteins for your research ...the Addgene blog Resources on Addgene.org Learn about the Michael Davidson fluorescent protein collection...

...resources on Addgene.org Learn about Addgene's viral service Learn about viral production Find viral protocols... By now you have probably heard about Addgene’s viral service but did you know that Addgene also performs... approach can be adapted by vector cores and laboratories alike as an easy and inexpensive method to improve...Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production." Biotechnology and ...

...focus on molecular pathways, I found myself curious about other routes – In terms of both types and topics...not resist applying for. Not that I knew anything about being an editor - that I learned on the job. The...science? Look at our graphics and design editors. How about condensing a week’s worth of the world’s scientific...the scientific publishing world and debate issues about how to improve the scientific publishing process...science communication professionals who are thinking about, for example, how to use new technologies to reach...

... your science with others.    My favorite quote about science ever comes from an interview in which someone...storytelling business like to say, science is all about navigating conflict. In literary terms, conflict...find that once I sit down and talk to a scientist about their research, these conflicts come out quite naturally... a simple question, like “Have you ever thought about how X works?”, or even by introducing a person that...devices, we sometimes forget the most important thing about science: It’s cool. Like bowties! Using science ...

...our Summer SciComm Series, where we’ll be talking about science communication, best practices in the field...research.   This summer, we’re making time to talk about my favorite thing - science communication (scicomm...endless.    Activity Example audience  Talking about your career as a scientist underrepresented minorities...students in AP biology classes Creating a podcast about genetics using dog breeds as an example adults ...get into the more exciting parts - learning more about science communication itself! See you next time!...

... also be collecting feedback from the community about how the antibodies work in various applications,...antibodies are plasmid-based and recombinant. Learn more about recombinant antibodies here. Finally, Addgene is...against PSD-95 from the Trimmer Lab. Information about the antibody, the target antigen, and performance...blog and Antibody Guide, which include information about immunofluorescence, conjugates, isotypes, and more...learn more or get started.  If you have suggestions about how to make the resource better or ideas for new...

...Twitter, you might have seen the video of her singing about the paper recently published by her lab and Mustafa...professor at the University of Freiburg. This paper is about a new tool called BLADE (blue light-inducible AraC....   Inspired by @UriAlonWeizmann, here my song about our paper recently published in @nchembio Title:... based on the concept of bacteriographs created about a decade ago. To create these images, the scientists...Additional resources on the Addgene blog Learn more about inducible promoters Visit the Plasmids topic overview...

...living as a scientist in Beijing, talking to people about taking beautiful photographs of their studies, reporting...to friends or dates or colleagues we tell tales about parts of our lives that tend to have a story structure...informational interview question: Interviewer: “Tell me about a time you had a conflict at work.” Interviewee:...situation down by opening a dialogue specifically about the project and contributions, rather than getting...defensive or getting into ‘he said; she said’. We spoke about it for a while and he eventually calmed down, apologised...

...resources on the Addgene blog Learn about company culture at Addgene Read about other lunch activities at Addgene...scientists bring biases to research and don't collaborate with others to bring diverse perspectives. Everyone...Science is a terrifying and equally good wake up call about the science of race."     - Joanne Kamens, Executive...easy reads, some are hard. Some I’ll never think about again, some I loved so much I’ll force them on everyone...

...Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources, equipment, and...discovery, and synthetic biology. Read on to learn more about the five awardees and their promising research projects... to the Addgene blog for updates and future collaborations. Many thanks to all of the awardees, all who...Additional Resources on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out...Expression Systems Synthetic Biology Resources Learn about Global Plasmid Distribution ...

... and we thought it would be a good time to talk about the amplification and verification processes we ... DNA. These reactions should allow us to obtain about 100 to 1,000 bacterial colonies per individual plasmid...from the count_spacers.py file from Feng Zhang’s laboratory. There are a number of other analysis scripts...you have any tips, input, or stories of interest about library use, let us know in the comments! Additional...NGS Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries Resources on Addgene.org...

...the Addgene blog Read more about COVID-19 databases and resources Learn about Addgene's work to share plasmids... availability of this strain from The Jackson Laboratory. Originally developed and characterized by Stanley...mouse strain is regaining popularity. The Jackson Laboratory, a nonprofit research institution, has been able...clinical manifestations, transmissibility, and laboratory studies of COVID-19: an observational study. ...

...distinguish between DNA and RNA. See our protocol about using UV absorbance to quantify DNA Fluorescence...absorbance based methods, this method doesn’t tell you about contaminating proteins or chaotropic salts. Capillary... instruments are available. Some last reminders about quantifying DNA In choosing your DNA quantification...Additional resources on the Addgene blog Read more about molecular biology protocols and tips Find the best...best way to elute and store your plasmid DNA Learn about the different grades of plasmid DNA preps Resources...

...frequency of use in black. This paper talks more about the development of the De-jargonizer.   Academic...with others which makes it easier to work with collaborators. Zotero is a desktop based program that’s compatible...episode 2 "Organizing Literature" : I found out about Zotero here Dear Grad Student podcast episode 2 ...Programming, & Productivity": This podcast talks about Mendeley, Zotero, and other citation managers and...the pomodoro technique": This podcast talks more about using the Pomodoro method in grad school Additional...

...during the day? We asked three lab heads how they go about selecting new hires. This is followed by some perhaps...visits the lab for a full day, gives a 1 hour talk about his or her interests, and spends one-on-one time...current members to share short-term goals and chat about awesome science. Important factors in the search..., the lab has a reputation for not entering collaborations. Alternatively, perhaps the lab publishes and...Medical School) for taking the time to speak with us about adding new scientists to their labs. Read the Other...

...Cancer, Inflammation and Immunity and learn more about “... recent findings in basic immunology, cancer-immune...curing cancer in cross-disciplinary projects and collaborations. Summarized by Professor Michael Karin from...This was followed by a talk from Professor Karin about alcohol and obesity as driving factors for chronic...got a chance to talk with Dr. Friedmann-Morvinski about tools used to analyse the tumor microenvironment...community, contact [email protected] to find out more about our free deposit and distribution service! References...

...often relies on antibiotic resistance. But what about identifying clones that contain your desired insert... restriction digest. The key is to be strategic about your enzyme choices. You’ll want to pick enzymes... for your PCR.  Find the details and learn more about colony PCR in this blog post.   Figure 2: ...Sanger sequencing or the other techniques mentioned above. In molecular biology, cloning a gene into your ... and tips Resources on Addgene.org Learn more about Sanger sequencing Read the Molecular Biology Reference...

...We also want to tell you about things you didn’t know you wanted to know about, like research breakthroughs... want to know more about! You can also suggest blog topics or even reach out about writing a guest post...technical skills, and experiences from thousands of laboratories that makes up our Help Center, blog, website...

... Learn more about research applications for antibodies in Intro to Antibodies Read about the Developmental...antibodies in your experiments, you may be curious about all of the different kinds of antibodies that are...Disadvantages of monoclonal antibodies As mentioned above, genetic drift is one concern when using hybridomas...Hybridoma Bank, a repository for hybridomas  Learn about monoclonal antibodies for immunotherapy Resource...

...reason behind this isn’t really hard to guess. Think about it like winter tires in different conditions: the...stable folding and efficient functioning. But what about placing the mesophilic hosts at 0°C? That won’t ...them which help foster a more productive and collaborative environment for research.  We’ve also involved...understanding of the societal ramifications of laboratory science, and how it may best be leveraged for...re always delighted to engage with potential collaborators and interested audiences. Please visit our team...

...in Huntsville explains it, a major goal in his laboratory is to understand the function of the non-coding...protein tagging. We spoke with Mendenhall and Myers about their new CRISPR tagging system, which they’ve now... antibody against them. There’s also a question about how specific those interactions are. This is solved...This design cuts out weeks of waiting and hands on labor. That’s a huge deal. With Addgene, now these can...system or who use and adapt our reagents to think about adding them to Addgene’s special collection of CRISPR...

...to understand the basis for cardiovascular and metabolic human diseases. They do that by studying patients...disease-associated genes. Addgene spoke to Musunuru about how the new kit works, the research the kit now ...number of genes involved in cardiovascular and metabolic diseases. We are knocking out genes that have ...to examine the effect of a gene on cholesterol metabolism. The TALEN kit is quite useful in generating ...disease modeling in stem cells. Addgene: Tell me about the history. How had investigators done this kind...

...include a note about where you met IRL (in real life) and perhaps what you talked about (or how good the... this rule, but not that many). I’m asked a lot about requesting connections to complete strangers for...have been able to tell when some start-ups were about to be bought by seeing certain groups of people ...using social media as a scientists Read blog posts about networking ...

...information about the contents of the autophagosome nor does it provide information about what is being...higher pH of the vacuole. Read on to learn more about prior methods for studying autophagy and how Rosella... (as opposed to giving more fine-grained detail about what proteins or organelles are being degraded) ...autophagy, but, like the protein degradation assay above, it’s slow and only measures bulk autophagy. Morphological...

... data and the list goes on and on. As you think about your career (and your future in general), keep in...in this post benefit from the applicant thinking about the process early, keeping good records, and planning...writing, compliance, core facility management, collaboration management, grant administration, clinical operations... in professional publications written by others about the alien's work in the academic field Evidence ...people who have not worked with you directly or collaborated on a project where they provided you with feedback...Celano, CEO Propel Careers. Lauren is passionate about working with scientific individuals to find exciting...

... posters can be. You have also probably thought about why certain posters resonate with you (or not). ...important to reflect on these impressions when thinking about crafting your own poster. What sets a scientific...Making your poster readable While we are talking about font, remember this: bigger is better! Your poster...gradient (as is shown in all of our illustrations above) so that the color fades to a lighter or darker ...conference attendance Read all of our blog posts about conferences ...

...resources on the Addgene blog Learn about RNP delivery Learn about CRISPR delivery methods for mammalian...Nanoblades pseudotyped with a mix of VSV-G and the baboon endogenous retrovirus Rless glycoprotein (BaEVRless...have lower off-target edits. Another great thing about Nanoblades? They’re easily produced in a BSL2 lab... guide Find CRISPR plasmids and resources Learn about viral production ...

...Have you ever been stuck googling about an antibody that will be essential to your new research project...high-quality antibodies for IHC. Working with collaborators and support from the Brain Research Through ...extensive library of monoclonal antibodies; Addgene collaborates with these companies to create and provide general...pattern should align with what the atlases (mentioned above) and literature suggest, providing an expectation...open-source database, the mAb3D Atlas will foster collaboration, progress, and innovation by allowing scientists...

...different SARS-CoV-2 spike variants. You can learn more about their research in this article. To see other plasmids... optimized. Browse the collection or learn more about it on the blog. COVID-19 research is happening fast...through plasmid sharing and more. As you’ve read above, some of these plasmids have been in the repository...Additional resources on the Addgene blog Learn more about mapping the SARS-CoV-2 - human protein-protein interaction... interaction Find tips for remote collaborations Browse all COVID-19 blog posts Resources on Addgene....

... 2015 conference invited me to attend and speak about Addgene’s experience helping scientists share plasmids... with the first keynote presentation. He talked about the impact of the Human Genome project on its 25th...of the meeting was how much we still don’t know about RNA. One talk that really brought this home was ...presented by Todd Lowe from UCSC. Dr. Lowe talked about his work on the 500+ human tRNA genes and, until... Udi Qimron from Tel Aviv University then spoke about using CRISPR/cas to address the growing clinical...

...the work I agreed upon (just as I would in my laboratory work). Committee members have to work professionally...to work with many different types of people both above and below you. During my time at the NPA, there ...further opportunities opened up to me. As I spoke about my work with the NPA, I was ultimately invited to...society in my scientific field - ARVO (as mentioned above). I became an official member of the Committee and...important for postdocs and graduate students to think about their career options, gaining transferable skills...

...all the current code on GitHub, ask us questions about it, or send us a pull request for improving it. ...) so that they can be used in the rank equation above. Cut-to-insert distance was fitted to experimental...below) so that it can be used in the rank equation above. Generally, the greater this distance between the...splits up the guide sequence.) If neither of the above are options, the algorithm silently mutates codons...Addgene blog Read our CRISPR 101 blog posts Learn about some considerations when designing gRNAs Find other...

...positive experience, I was still incredibly nervous about leaving academia. A lot of academics tend to view...which may take a longer time to acquire. Learning about drug discovery: Unless your academic lab is closely...information. Working as a team: Industry is all about teamwork. A single individual could not bring a ...someone with whom you can have an in-depth discussion about your project might be challenging. In an academic...contacts to get that referral and find out more about the company’s program. Once you’ve found a few positions...

...Until then, you can only wonder what I am talking about. How do researchers use physical models of protein...students work closely with local researchers to learn about their research while creating physical models of...model is an effective teaching tool. You can read about Jacob’s initial reaction to this model on his blog...will wake up in the middle of the night dreaming about the conformational gymnastics that this protein ...Additional Resources on the Addgene Blog Learn All about CRISPR Use CRISPR for Genome Visualization Tips ...

...If you follow CRISPR research, you know all about using non-homologous end-joining (NHEJ) to make deletions...plasmids, GFP knock-in cell lines can be created in about a month and a half, without the need for complicated...more predictable than NHEJ. As seen in the figure above, short (5-25 bp) regions of homology flanking a ...Directed Repair and Non-homologous End Joining Read About Other Genome Engineering Tools like Sleeping Beauty... Additional Resources on Addgene.org Learn All about CRISPR on Our CRISPR Guide Pages Find CRISPR Plasmids...

... widely available, so you’ll only have to worry about cloning your transfer plasmid. The transfer plasmids...typical insect cell preparations, which we talk about below. Processing The viral supernatant is typically...available, so the amount of cloning required is about the same between insect and mammalian systems. Baculovirus...dead cells and cellular debris. While adding time (about three to four days), this second round provides ... can skip the first round of production, saving about a week of work. An important note is that this is...

...our tissues unimpeded. Andrew and Maria dreamed about the possibilities of a magnetoresponsive fluorescent...of improved response. Then, just when Andrew was about to suggest she deprioritize the project, Maria’s...with a strong commitment to exploration and collaboration. Andrew is a physicist with a background in ...potential applications.  Andrew is a frequent collaborator and is happy to give advice and support for ...interested in working on these questions or collaborating, the York Lab is eager to hear from you. Find...

... think much about management skills during your scientific training. I never thought about management ...open mind. First let’s clear up a misconception about being a manager: yes, being a manager means being...you should be doing : writing a grant, thinking about the next project, reaching out to other teams, expanding...buffer them from unnecessary pressure “coming from above”, which is when other skills such as negotiation...

...republished here with permission. Quilt is a collaborative database for genomics. In this article, Quilt...tri_methylation In the next section we’ll apply the above formula to a real-world experiment. We’ll start ...discovery and therapeutics. We’ll have more to say about clinical applications of CRISPR in the next section...Aneesh Karve is CTO at Quilt Data. Quilt is a collaborative database. Aneesh's research interests span machine...on Your CRISPR Background with CRISPR 101 Learn about CRISPR Delivery Methods Validate Your Genome Edit...

...Base Transversion Editors Learn about base editing for plants Read about four base editor reporters Additional... The BE3 system increased editing frequency to above 30% for a variety of targets in human cell lines... Liu lab created BE4max and AncBE4max mentioned above, they also made an adenine base editor with improved...cell therapy applications. Dual base editors What about combining the function of both editors into one?...also RNA base editors, which you can learn more about in this blog post: CRISPR 101: RNA Editing with ...