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We narrowed to 753 results for: ELP

Showing: 321 - 360 of 753 results

  1. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...current methods, understanding the differences can help you make an informed decision. Mammalian cells Mammalian...on these differences in viral production and will help you decide which is right for you. Table 1: Mammalian...

  2. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...they have collected a variety of data that might help formulate some hypotheses. They are excited to see...troubleshooting tips and works with other labs to help them adopt the tools his group develops. And though...

  3. Pooled Library Amplifications

    Type
    Blog Post
    ...is your desired library coverage, since this will help decide what transformation efficiency you need to...plates). In general, amplification on plates can help to ensure that diversity of the library is maintained...

  5. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...AAV-RPE65 treatment, famously visited Congress to help increase awareness about the potential of gene therapy...and colleagues at MEEI co-founded a non-profit to help push forward candidate therapies for these diseases...

  6. DNA Purification Without a Kit

    Type
    Blog Post
    ...fragments You might be wondering, “How is milk going to help me extract DNA from agarose gel?!”   The DNA gel... it solubilizes both DNA and agarose, and 2) it helps DNA stick to the glass. After binding DNA, the glass...

  7. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...density. In some cases, nanobodies have been found to help improve label density (Mikhaylova, et al. 2015)....approaches. Since it arrived on the scene, ExM has helped researchers gain a deeper understanding of neuronal...

  9. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...improvements to yeast transformation protocols also helped establish the yeast two-hybrid (Y2H) system as ...for the modification in the assay host strain can help. In order to reduce the chance that the configuration...

  11. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...reliable results from CRISPR edit data requires the help of robust software tools. As a consequence, a critical...detected edit types in the sample. The visualization helps the researcher see which of the multiplexed sgRNA...

  12. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ... lentiviral vector will infect, pseudotyping can help. In pseudotyping, you produce viral vectors using...stable and can withstand ultracentrifugation which helps scientists achieve a high titer (reviewed in Joglekar...

  13. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...RepZ to drive its own replication. Like RepA, RepZ helps recruit host initiation proteins to the plasmid ...Plasmids 101 Posts Get practical molecular biology help with our Molecular Biology Reference Pages   ...

  14. How to Write a Scientific Review Article

    Type
    Blog Post
    ...stuck trying to write one, hopefully this post will help you get things moving - remember you're becoming...Year) in the document while writing, because it helps you later on to remember where you read particular...

  16. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...For us, transient leaf transformations are a great help in screening candidate genes and speeding up research...transformations, but we hope that these tips might help to get you started. Once you have established a ...

  17. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...gene modified mouse lines. Hopefully this post will help get your in vivo research up and running as soon...Resources on Addgene.org Find Software Tools to Help Design You gRNA Find CRISPR Plasmids for Use in ...

  19. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...are a few simple troubleshooting measures that can help get you back on track: Compatibility: Double-check...inducer or adding an affinity tag such as GST may help with solubility issues. We want to hear about your...

  21. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ... probes, we decided to write this quick guide to help researchers choose the probe for their needs. You...assays with other FPs.   We hope that this post helps you in getting to know the state-of-the-art NIR ...

  22. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ... phage display library with plate-bound antigen helps select for high affinity scFvs. Bound phage are ...expression in the presence of molecular chaperones that help scFvs fold properly in the cytoplasm (Sandomenico...

  23. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...muffled voices beneath masks, humans rely on mouths to help them hear and read emotional cues. We had two options...written and video protocols at Addgene? If you need help in the lab, we have you covered.  ...

  25. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...biotinylation reagents to help with the specific elution of biotinylated proteins. For help determining which ...

  28. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...publication, improving your general writing skills will help you in your academic publishing goals and make you...reach out to your peers and other mentors who can help you. You Can Talk to Your Editors I wish I had...

  29. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...modifications to DNA or histones, the proteins that help package DNA, were associated with gene expression... CRISPR has made targeting much easier! With the help of guide RNAs, fusions between Cas proteins and ...

  30. A Control for All Seasons

    Type
    Blog Post
    ...world where so much is out of your hands, it’s helpful to focus on something controllable, like experiments...results of a single mouse. Biological replicates help you to determine which responses arise from drug...

  31. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...options to consider, and the following factors will help you determine which one(s) are candidates for your...are a number of optogenetic tools now available to help light your path! Resources and references Recommended...

  32. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...You’ll need to consider if such properties could help, or harm, your experimental goals when designing... other posts in our Viral Vectors 101 series can help guide you with these, so keep reading! Good luck...

  33. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...deposit your piggyBac™ transposon vectors with us to help us expand this useful resource. While Addgene cannot...nucleases. Mathew applies his specialized knowledge to help researchers identify the right technologies and ...

  34. Antibodies 101: Validation

    Type
    Blog Post
    ...likely won’t work in a given application is just as helpful as knowing when it will. And remember, antibodies...others in the community can benefit and we can all help improve antibody reproducibility!     References...

  35. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...So, labeling a HaloTag or SNAP-tag construct can help you better detect dim structures or low levels of...are comfortable with it or can find a colleague to help you, synthesizing your own ligands can open up more...

  36. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...polybrene (protocol) or DEAE dextran (protocol), which help the viral particle bind to your target cells. If...packaging cell lines to avoid recombination leading to helper virus production." Molecular and Cellular Biology...

  38. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...If this sounds like you, then flow cytometry can help you overcome these challenges and make these types...except one. The fluorescence-minus one panel will help you set up gating during data analysis.  We hope...

  39. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...what we discuss. If you can think it, MoClo might help you achieve it! MoClo applications and specialized...2017). If you can dream up an experiment, MoClo can help you achieve it! More recently, a CRISPR MoClo kit...
Showing: 321 - 360 of 753 results