We narrowed to 731 results for: Sis;

...the accompanying sequencing data collection and analysis eventually outpaced our software, even with continuous...singular purpose (ex. adapter trimming, quality score analysis, k-mer counting, or sequence assembly), but the...would handle plasmid sequences on a 96-well plate basis, but for rapid testing and refinements, we specified...

...that a plasmid can transfer to, replicate in, and persist in. Some plasmids have a narrow host range, while...host range recombinant vectors Replicons, which consist of a plasmid’s ori and all of its control elements...scientists can incorporate interchangeable antibiotic resistance cassettes (Prior et al. 2010), inducible promoters...

... is the person you report or talk to on a daily basis who will help you get on your feet with techniques...accordingly in case you have questions or need a little assistance at some point during the procedure. Be an active...everything is magically made up by helpful TAs or assistants, and sitting out on the bench waiting for you...

...of its parts. Elements such as the antibiotic resistance gene and the origin of replication are necessary...elongation factor 1 alpha Constitutive  Tends to give consistent expression regardless of cell type or physiology...expression, but may vary by cell type. Tends to resist promoter down regulation due to methylation or ...

...contributed by guest blogger Joachim Goedart, an assistant professor at the Section of Molecular Cytology...conditions needs to be examined on a case-by-case basis. GFP is sensitive to acid The chromophore of GFP...blogger, Joachim Goedhart! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...

...promiscuous biotin ligase (Kim et al 2016). BioID2 consists of a humanized biotin ligase from A. aeolicus,... assemble when in close proximity. Split-BioID consists of a split BirA* molecule that can reassemble ...compared to the standard E. coli BirA* mutant. RapID consists of this mutant BirA* and an RNA component that...

...ability to reassemble fragmented genomes is the basis of several split vector approaches developed in ...transgene expression from split vectors has been inconsistent. A greater understanding of basic AAV biology...vector strategies and lead to greater and more consistent transgene expression. References: Allocca M...

...s group created Spinach2 using site-directed mutagenesis of Spinach. In doing so, they identified the ... IV, were then identified through microfluidic-assisted in vitro compartmentalisation for their high binding...PMC3852148. Warner, Katherine Deigan, et al. "Structural basis for activity of highly efficient RNA mimics of green...

... social science to an audience that I thought consisted of all biologists. The social scientists in the...to them with an answer, and do so if you can. 9. Resist the urge to say everything I allow 1 slide for ...topic or story is all you need, if you do it well.  Resist the urge to show all or even a lot of data.  I ...

...must be recoded to ‘TGA’ using site-directed mutagenesis. In the C321.ΔA strain, this TGA will be recognized... formation: the last puzzle of aminoacyl‐tRNA synthesis." FEBS letters 462.3 (1999): 302-306. PubMed PMID...Sauerwald, Anselm, et al. "RNA-dependent cysteine biosynthesis in archaea." Science 307.5717 (2005): 1969-1972...

...C-terminal HA tag. Selectable and stable: A puromycin resistance gene makes stable cell line creation a breeze...supernatant and selected with puromycin. Puromycin-resistant cells were fixed and labeled with primary antibody...the genome (Figure 4). Figure 4: STITCHR consists of a nicking Cas9 fused to a shortened R2Tocc ...

...particularly interesting as I had just completed my thesis on the study of e2Fb, which is a tumour suppressor...but I really struggled with the large scale data analysis, as this is increasingly reliant on programming... to use diagnostic tool for the detection and diagnosis of different types of pancreatic cancer and highlighting...

...coupled with iterative rounds of selection and mutagenesis to refine antibody performance. If you are interested...L. (2019). Antibody Structure and Function: the basis for Engineering therapeutics. Antibodies, 8(4), ...Wilson, D. S., & Keefe, A. D. (2000). Random mutagenesis by PCR. Current Protocols in Molecular Biology...

...as a control. DNA was analyzed by PAGE. The basis of the Surveyor assay is that Surveyor nuclease ...edit. Using Surveyor nuclease followed by gel electrophoresis, these mismatches are indicated by the presence...Figure 4). 5. In theory you could use densitometric analysis to quantitate the intensity of the bands on your...

...plasmid vectors and/or rely on cumbersome in vitro synthesis of single guide RNAs (sgRNAs), are time-consuming...reagent preparation in the lab. Figure 3: csRNP-assisted epitope tagging is scalable to 96-well format...., Gogolok S et al. 2017. Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic...

...depends on it. Deanna McLafferty is an editorial assistant and activist. Washington, DC, USA 38.9072°N 77.0369...scientists – including global warming skeptics – insisted that more data and research funding are critical...trying turn a few exoplanets into a successful PhD thesis. We use the same atmospheric physics to understand...

...undesirable side products generated during chemical synthesis of these molecules. To make large amounts of pure...applications, but there are more! Engineering disease resistance through gene stacking, increasing production ...Generation of 8× Nicotiana Benthamiana and 12× Arabidopsis Mutants.” The Plant Journal, vol. 106, no. 1,...

...number. First you generate four unique kanamycin-resistant plasmids, each containing a different gRNA target... all but one of your gRNAs into spectinomycin-resistant plasmids pX330S-2 to pX330S-(last gRNA number).... Yin, Linlin, et al. “Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs...

...engages the prime editor protein. The prime editor consists of a Cas9 nickase fused to a reverse transcriptase... domain for optimization. They developed phage-assisted evolution strategies to evolve more effective ... J., Osborn, M. J., & Liu, D. R. (2023). Phage-assisted evolution and protein engineering yield compact...

...creation of improved fluorescent proteins through mutagenesis. Chapter 6 - The future of FRET and fluorescent...guest, Joachim Goεdhart! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...

...career landscape and there is insufficient (or no) emphasis on developing transferable skills to enable pursuit...recent discussions of this apparently worsening crisis recently appeared in eLife: "A Fair Deal for PhD...

...you're transforming a plasmid with ampicillin resistance.  After a plasmid is introduced to bacteria, ...culture without antibiotics to give the antibiotic resistance gene time to be expressed.  However, you can ...

...Saftic) to educational gifs created by the Amoeba Sisters. Here’s one example of how they “bridge the jargon...jargon gap using gifs”: https://amoebasisters.tumblr.com/post/139944465237/lactose-around-we-can-make-...

...lectures targeting three broad concepts: the genetic basis of disease, cancer, and viral infections. An interactive...science, but they didn’t seem to mind! Attendees consistently mentioned how pleased they were to learn about...

...perturbations that may play significant roles in cancer pathogenesis. Their work culminated in the first functional...lentiviral constructs using the High-Throughput Mutagenesis and Molecular Barcoding (HiTMMoB) technique (...

...genes as drivers of peritoneal mesothelioma tumorigenesis. They plan to use CRISPR and lentiviral plasmids...peritoneal mesothelioma cells lines and assess their tumorigenesis. These experiments will serve to validate these...

...they also went in search of genes involved in resistance to the cancer drug vemurafenib. Enriched libraries... screen for genes involved in bacterial toxin resistance, identifying 4 previously unknown genes.  “With...

...Optogenetic Tools Like pDawn  Making cassava plants resistant to cassava mosaic virus Dr. Fiona Robertson, University... cry genes from the soil bacterium Bacillus thuringiensis and analysing the insecticidal potential of ...

... evolution took over (with a little mutagenic assistance). Quicker CAST activity allowed certain mutated...evoCAST to the Liu lab’s own PASSIGE (Prime-Editing-Assisted Site-Specific Integrase Gene Editing), which combines...

...and undigested samples of the clones on an electrophoresis gel in order to differentiate which clone had...Once we had gotten our results back from the electrophoresis, my manager and I were unsure if there were...

...  Joining the Addgene crew!  This pattern was consistent: everyone I spoke with at Addgene, no matter ...presentation.  My first time in the lab! I got to assist production on a protocol video. Don't worry - no...

...microtubules. Tanenbaum et al. then tested their hypothesis that the lower expression levels of SunTag constructs...suitability in your experiments on a protein-by-protein basis. However, since many of Addgene’s SunTag plasmids...

...University of North Carolina (plasmids at Addgene). It consists of two domains, LOV2 and Zdk1, where Zdk1 binds...RNA localization in mammalian cells. These tools consist of cobalamin-binding riboswitches that bind to ...

...We believe that this simplified sequencing and analysis approach can be easily incorporated into standard... A Rapid and Robust Assay for Batch-to-Batch Consistency Evaluation of AAV Preparations. Molecular Therapy...

..., 2020. Now that we know all about antibiotic resistance genes, let’s consider another basic element of...for single- and multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12...

...neuronal circuit tracing.     Marcus Leiwe is an assistant professor at Kyushu University. He has a particular...resolution using chemical cocktails and computational analysis." Cell 157.3 (2014): 726-739. PubMed PMID: 24746791...

... dynamic process which involves autophagosome synthesis, delivery of materials to be degraded to the lysosome...mammalian dual-emission autophagy biosensor. Its resistance to lysosomal proteases is key to its pH-dependent...

...perception of head injury risk. In completing a master’s thesis in science communication, I had the chance to carry...necessary to inform the experimental aspect of my thesis: a survey instrument designed to measure how individuals...

...Instead, it targets a specific audience - giving consistency, purpose, structure, and intent to the messaging...your audience, and their expectations of you, consistent and clear.   And now that we’re all on the same...

...detection of the tagged protein. These peptides consist of as little as 8 or as many as several hundred...10.1007/s00253-002-1158-6 Baker, M., Reproducibility crisis: Blame it on the antibodies. Nature (2015) 521,...