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Showing: 401 - 450 of 929 results

  1. Addgene’s GIPHY Page is Here to Help You Express Yourself (and Your Gene of Interest)

    Type
    Blog Post
    ...We’re making some moves over here at Addgene...and they’re looping? What’s the deal with looping...GIFs? We have answers! via GIPHY   We’ve been working hard to create the most informative and entertaining...protocol videos. via GIPHY   If you’re also looking for that perfect GIF for your TikTok or Instagram...us know on Twitter @addgene! Addgene is always looking for new ways to help you celebrate those wins in...

  2. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ...If you’re looking for an antibody that works for your experiment, you may be overwhelmed by options —... — or not know where to start looking to find one. Luckily, there are websites that can help: Antibody...antibody catalogs at the same time.  If you're looking for an antibody, especially a validated antibody...antibody quality throughout research, consider checking out the Only Good Antibodies community on LinkedIn...

  3. 2018 #DeckTheLab Contest Winners

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    Blog Post
    ...always gets done this time of year 😁 we stopped working on our reports/lab stuff for a few minutes to create...ladies #research #cardiovascularresearch #parkinsonsdisease A post shared by Ladies in labcoats (@ladiesinlabcoats...lab went all out to transform their lab into a walking tour of Buddy the Elf’s journey from the North ...fireplace scene.  Got our @EmoryChem lab office looking festive 🎄🌟🎁 @Addgene #DeckTheLab pic.twitter.com...

  4. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    ...Seeding Labs Plasmid Grant. For this grant, we’re working with fellow nonprofit, Seeding Labs, to distribute... health. 40:17 - 44:14 : Microbiome research - working with bacterial communities both on the human body...microbiome expertise. Many thanks to Kwabena Duedu for taking the time to sit down and talk with us.  Kwabena...the Addgene, Seeding Labs Plasmid grant and is working on a variety of projects in Ghana.     Special ...

  5. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    ...material so that you can more easily find what you’re looking for. Whether you’re joining us for the first time...the blog that will give you a better experience. Looking for something? Use the navigation menu. The new... the top of the screen and type in what you’re looking for. After you search, you’ll see the blog articles...bloggers Like a blog post from a certain author and looking for more from them? Check out the new author pages...

  6. Quick Guide to All Things Lentivirus

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    Blog Post
    ...competent lentiviruses (RCL) especially if you are working on HIV positive human cell from the clinic. The...extra care has to be taken especially if you are working with proto-oncogenes or with human samples that... is now used as it shows rapid gene expression kinetics as compared to the Tet-off system. One drawback...one with either tTA or rtTA (FUW-M2rtTA). In this kind of approach, a population of cells might express...transgene resulting in low inducibility and potentially making it difficult to interpret your results. This can...

  7. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...when did you start thinking about them? 18:30 - 19:54 : How to think about picking a post-doc project ... you leverage your community, think hard about picking a good mentor, and begin considering career options...Mentoring" We'd like to thank Niroshi and Ben for taking the time to sit down and talk to us and wish them...

  8. Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis

    Type
    Blog Post
    ...a startup and back to academia. Chapter 1: How working at a startup prepared Tom for an academic career...more complicated biological devices. Chapter 6: - Making research go faster through Open Access. Chapter...investigate synthetic biology at Boston University, working in one of the founding groups of the field under... Plasmids 101: Golden Gate Cloning Learn About Working at a SynBio Startup in Our Interview with Michael...

  9. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ... including that it’s never too early to start networking! Learning about careers at Addgene Afterwards...popular, with the students excited to see a real working lab full of busy Addgenies preparing plasmid samples...impressed our guests, as did how much work is put into making sure scientists all over the world know that we...Advice from Our Bloggers Learn How to Improve Your Networking Skills  ...

  10. 500,000 Plasmids Shipped and Counting!

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    Blog Post
    ...providing quality control and customer support, making plasmids easy for you to find and request in our... Addgene? Here's an inside peak into the daily workings in Addgene's lab. We ship individual plasmids ...Stabs are organized by shipment and moved to our packing area.Using the barcode system, the correct plasmids...necessitate an office move. We've got ~50 Addgenies working to ship plasmids, analyze QC sequence, answer scientists...

  11. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type
    Blog Post
    ...Brownstein, an Addgene board member. When I was working in Human Resources at Harvard Medical School, Joanna...Joanna was my colleague. She mentioned Addgene was looking for someone with HR expertise to join their newly... her forever home. I feel blessed that I’m not working so that I’m available to spend time caring for ...for my mother. For fun, I enjoy pilates, jewelry making, reading, sailing, and gardening. Learn more about...

  12. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...expression cassettes (Shaw et al., 2023). Those looking to express high levels of their gene of interest...more! Engineering disease resistance through gene stacking, increasing production of a protein of interest...requirements and editing abilities, such as base editing, nicking, cleaving, etc. The possibilities really are endless...mammalian systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate system was designed to work in....2024.100519. Chiasson, David, et al. “A Unified Multi-Kingdom Golden Gate Cloning Platform.” Scientific Reports...

  13. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ... don’t want to spend months doing surgeries and making fiber optic cables only to hook up your lasers ...alter the light spectrum for activation and the kinetics of opsin activity/inactivity. Dr. Deisseroth has...(not expressing the opsin) are also available. Picking your opsin  You must consider whether you wish ...neuronal firing, the cell-type specificity, the kinetics of the opsin variant, and other particularities...the promoter of the cre recombinase while still taking advantage of the robust expression via the promoter...

  14. Editor's Choice, September 2016

    Type
    Blog Post
    ...Francisco International Airport listening to the Lion King soundtrack and writing this post, it is my pleasure...what new post contributed the most to this record breaking month and to find other posts that deserve a second...Navigate a Scientific Enterprise in Flux CRISPR Kinome Libraries Available: Pooled and Individual Plasmid... Research Community Grad School Advice Part 1: Picking a Lab and a Project That’s it for now. As I indicated...

  15. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    ...Tools for Making Scientific Graphics by Beth Kenkel Find a round-up of tools you can use for making scientific...we're gearing up to start a new decade, we’re also looking back at the last year of scientific discovery. ...Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection by Joachim Goedhart Guest...

  16. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    ...at a lindy hop swing dance event,” Zheng says (networking can happen anywhere!). The pair quickly teamed... in November 2017. #Phage researchers! I am working with a team to get Burkholderia cepacia phages ...must recognize a specific part on the bacterium making each phage is very specific. Some infect only one...about scientific sharing Find blog posts about networking Additional resources on Addgene.org Find molecular...

  17. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    ...experiment. (And you’re certainly not alone – we’re all looking for high-quality antibodies and reagents that work...and sorting. Reference images can be viewed by clicking on the links in the "Agree" and "Disagree" columns...associated metadata adapted from the Affinity Reagent Working Group of the Human Biomolecular Atlas Program (...community when designing the IBEX Knowledge-Base, seeking to create an iterative process that is constantly...

  18. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    ...excited to finally share with you what we’ve been working on behind the scenes here at Addgene.  Addgene's...messaging, as well as simplifying the page, and making it all look clean and easy to use.  Easily search...global navigation and menus over the past year, making it much easier to get around our site.  Community...smile, but occasionally you might notice Blugene looking sad, especially if an experiment doesn’t go as ...

  19. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ... search. This is especially true of scientists seeking a first job after the academic bench. Utilizing... the job posting instructs even if you have a networking connection that will be helping (see below). ...strengths in your resume and don't be shy about asking for help. The worst thing that can happen is someone... scientist networking...

  20. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ...,” said Addgene’s Lianna Swanson, who has been working with members of Davidson's lab to coordinate the...For more information on choosing a backbone and making fusions, see this blog post from Joachim Goedhart...Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection.  mEmerald plasmids and many... many more! Baird has a helpful tip for anyone looking to peruse the collection without getting overwhelmed...

  21. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...faster kinetics. The opsin ChETA, for example, is a ChR2 opsin mutated to have faster off kinetics and allow...set of tools therefore depends on what you are looking to study. Figure 1: A schematic showing the...receptors in the brain. One way to address this is by working with organisms with light accessible brains, such...

  22. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...your plasmids are distributed. Next month, we are making two changes to the policy that will make the process...the plasmids online for distribution instead of risking leaving them on hold indefinitely. Of course, the...citation and release the hold. Increasing impact Making the plasmids available as soon as your paper is... paper comes out later. Or, if a project isn’t working out as planned, but you still hope somebody will...

  23. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    ...paper they’re reading has been linked in a ReFigure making it easy for others to discover your insights on...researcher Christa Osuna collated Zika case studies, making a meta analysis available to the community on ReFigure...PubMed Central, eLife, PLOS and Figshare but we are working on expanding our reach. We can’t save you the hard...career researcher and cofounded ReFigure with Joe Akin. She envisions a world where the impact of teaching...

  24. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...and you are smart, you can be a good manager. The kind of smarts and the type of skills that it takes to... from being included in organizational decision-making. It is imperative that employees are thanked for...manager keeps these factors in mind at all times when making decisions, assignments and polices. Sometimes the...priorities for advancement in science Have their own working schedules and may not necessarily be comfortable...

  25. Advice for Choosing a Research Project

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    Blog Post
    ...persuing milestones and working hard. Being patient, being selective, and talking to peers and mentors will...with the current technology to identify a problem, asking what approach will I take and what can I learn,... Dr. George Church (Harvard Medical School) for taking the time to speak with us about adding new scientists...

  26. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...whirlwind of skill acquisition. Classes covered filmmaking, podcasting, philosophy of science, creative ...life of Srinivasa Ramanujan, to be precise) and remarking to a classmate that, if this was one day required...communication is an equally fruitful endeavor for those embarking on the route towards academia. Many of those entering...master's degree in 2020, his research explores risk-taking behavior among collision sport athletes, as well...

  27. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...Working on the scientific support team at Addgene, I get to see everything that can go wrong with a plasmid...DNA yield. Test multiple, small colonies After streaking LB agar plates, you may notice that there are ...recombined product tend to grow faster, so we recommend picking the smaller colonies which are more likely to carry...the recombined vector backbone, you can try re-streaking for single colonies and testing multiple preps...

  28. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...immunohistochemistry images that are crisp, bright, and lacking in non-specific staining can be a challenge in ... mixing your primary and secondary antibodies, blocking excess secondary with serum from the same species...problem.  Pre-block with Fab fragments (Figure 2C) Speaking of Fab fragments… Another strategy for species-on-species...0022155413511620  Lu QL, Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody...

  29. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...just on the disease front. Viruses survive by hijacking the machinery of the cells they infect. Once they...proteins. This is, for the intrepid researcher looking for molecular biology tools, a very useful trait...Created using biorender.com   If you’re new to talking about viruses, here’s a quick tip on terminology...reproduce only in experimentally controlled conditions, making them far safer to work with than virions. The four...

  30. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...inclination to be a teacher was looking for an outlet. I started asking people: why do you do what you ...bioscience into context, while at the same time working through the details of experimental design and ...of science communication professionals who are thinking about, for example, how to use new technologies...

  31. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...features provide extra layers of risk management when working with viral vectors. Typically, one day after transfection...particles are secreted into the cellular media, making viral collection fairly easy. Producer cell media...transfect’ cells lines are amenable to transduction, making it a logical choice for efficiency. Where viruses...with alternative methods. Ready, set, transduce! Making virus can sound daunting, but it is easier than...

  32. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...Plasmagenes? In the 1940s and 50s, scientists were working to understand genetic cytoplasmic factors that ...and we’ve been using "plasmid" ever since! From napkins to notebooks Although discovered in the early 1950s...initiated at a Hawaiian deli in 1972. Using a deli napkin for paper, a small group of scientists including... curate, and distribute them all in the name of making it a little bit easier for scientists to conduct...

  33. K. phaffii: Rising to the Occasion

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    Blog Post
    ...established in yeast, including a widely used Parkinson’s experimental model. In fact, approximately 25%...phaffii is highly efficient at protein secretion, making harvesting recombinant proteins a cinch. This is...it doesn’t secrete its own endogenous proteins, making the recombinant protein its major secretion. Beyond...players in the game (even beyond K. phaffii ) worth looking into whether you’re just dipping your toes into...

  34. Science Career Options

    Type
    Blog Post
    ...Will the job require public speaking? Will you be leading a group or working as part of team? Will you .... It is all too easy to get tunnel vision when working towards a specific goal, and when you realise that...often be misleading. Key aspects to determine are working hours (9am-5pm Mon-Fri or overtime and weekends...

  35. Plasmids 101: NGS Quality Control for Pooled Libraries

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    Blog Post
    ...amplification looks like, but by looking at these kinds of graphs, making sure few/no plasmids have dropped... the key points you’ll need to remember when undertaking library amplification and sequencing. Maintain...

  36. Plasmids 101: Mammalian Vectors

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    Blog Post
    ...will define positive selection as the means of picking up positive traits (i.e. the plasmid contains a...toxin), whereas negative selection would be the picking up of a negative trait (i.e. the plasmid contains...Conferring Resistance Cell Types* Mode of Action** Working Concentation*** Blasticidin bsd  HeLa, NIH3T3...transfection when creating stable cell lines. Looking for Mammalian Expression Plasmids? Check Out Addgene's...

  37. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ... with translatable skills: working with data, working with people, working with complex processes Arm ...Ginkgo Bioworks, our software development team was working on lab operations software for next-gen sequencing...

  38. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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    Blog Post
    ...spread of COVID-19. That’s why we’re proud to be working with Ginkgo Bioworks to synthesize and share over...analyzed first thing the following day. Nothing like waking up to fresh data! These same plasmids are then ...ensuring our rigorous QC standards are met, we are taking a unique hybrid approach compared to our standard...high throughput platforms, Addgene and Ginkgo are striking a balance by modifying certain processes in both...

  39. Sweating the Small Stuff: Details in the Lab

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    Blog Post
    ... the experiment in my head, double-checking details, or just taking a moment to relax. During really stressful...worth doing, and doing well, so make sure you’re taking time to do them carefully and thoroughly. Have ...however, many ways for you to reduce the chances of making an error. Try a variety of things, including (and...

  40. Targeted Mutagenesis with EvolvR

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    Blog Post
    ...and molecular biologists use to tinker with DNA. Making changes to a DNA sequence can help scientists identify...components: the error prone PolI3M that’s fused to a nicking Cas9 (nCas9). Similar to mutagenesis with error...error-prone polymerase (PolI) that is fused to a nicking Cas9. A guide directs Cas9 to a site of interest...changes promote nCas9’s dissociation from DNA after nicking and yielded an enhance nCas9 (enCas9) EvolvR with...

  41. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...gate which selects anything to the right of it, marking it as GFP+. Quantification for such graphs could...plot! The dreaded smear - fear not! When you are looking to study the level of protein expression, instead...Figure 2). These smears tend to occur when you are looking at proteins that are expressed regularly in cells...percentage positive or negative in your dot plot, looking at a histogram vs antibody plot would be better...

  42. A Deep Dive into BioBuilder

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    Blog Post
    ...question more and more students are asking. Teachers are also asking, “How do I engage my students in real-world...outcomes by emphasizing the importance of critical thinking, persistence, teamwork, and communication. And... the only informal synthetic biology club of its kind, running from start to finish during the academic...

  43. Plasmids 101: Screens vs. Selections

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    Blog Post
    ...work best for your scientific question? If you’re thinking, ‘wait, those terms mean different things?!’, ... to clearly define what type of factor you are looking for and what process or outcome that factor functions... advantage – that is, you are not specifically looking at cells that can survive. Instead, all manipulations... screens are similar to CRISPR, but instead of knocking out the genes of interest (or the whole genome...

  44. CRISPR 101: Non-Homologous End Joining

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    Blog Post
    ...Ku then acts as a scaffold for recruitment of a kinase (DNA-PKcs) and a two subunit DNA ligase (XRCC4-...heterogeneous when constrained by sequence identities in flanking sequence (“microhomologies”). NHEJ doesn’t obligatorily...Renouf B, Renaud J-B, Sallmyr A, Ruis B, Oh S, Tomkinson AE, Hendrickson EA, Giovannangeli C, Jasin M, ...Inoue A, Matoba S, Zhang Y, Zhang F (2013) Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing...

  45. Dos & Don'ts When Publishing a Scientific Manuscript

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    Blog Post
    ...Developmental Editor at F1000Research.  With many changes taking place in the world of science publishing, and an...include in my paper? More and more journals are asking for detailed information on lab resources, such...enough information to replicate your experiments, making research more reproducible and your results more...journals as possible at the same time. Take your time picking the appropriate journal and explain in your cover...

  46. Delivery Methods for Generating iPSCs

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    Blog Post
    ...note: in this table, reprogramming efficiencies ranking as low, medium or high is based on the reprogramming... pluripotency of mouse cells typically involves making chimeras by injecting iPSCs into blastocysts and... injecting iPSCs into immunodeficient mice and looking for tumors that contains cells from all three germ...of iPSCs is the number one priority, with safety taking lower priority. Genomic integration is less of ... and Juan Carlos Izpisúa Belmonte. "Methods for making induced pluripotent stem cells: reprogramming à...

  47. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    Blog Post
    ...promote tumor durability instead of repressing them, making it even harder to cure cancer. To understand how...factor, vascular endothelial growth factor, and interleukin-6 (Witkiewicz et al., 2009) which contribute ...metastasis of tumors through inciting inflammation. Speaking of inflammation, a noteworthy cell type in the...Witz, Isaac P. "The tumor microenvironment: the making of a paradigm." Cancer Microenvironment 2.1 (2009...

  48. RaPID Detection of RNA-protein Interactions

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    Blog Post
    ...like cross-linking immunoprecipitation (CLIP) identify RNA-protein interactions by cross-linking RNAs and...enrichment compared to E. coli’s BirA*. Faster labeling kinetics also means that BASU could potentially be used...RaPID RaPID sounds like a nifty technique, but what kinds of questions can it help answer? Ramanathan et al...

  49. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...with a heterozygous 4 bp deletion in MYBPC3 donated skin, blood and semen samples. Ma et al. designed plasmids...of only 27.9% and homology-directed repair (HDR) taking place in 41.2% of edited cells. The results were...CRISPR editing. Conclusions & implications One striking feature of this paper is the failure of the embryos...comprised of individuals with varied backgrounds. Talking about this study with non-biologist friends Like...

  50. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...autophagosomal membranes, so tagging it with GFP allows for tracking the localization or accumulation of pre-autophagosomal...an autophagy-independent manner. Additionally, tracking Atg8p or LC3 doesn’t provide information about...easier to study the mechanisms behind autophagy by tracking what’s being transported to the yeast vacuole ...vacuole (i.e. cytosol, mitochondria), rather than tracking generic autophagy markers. It’s particularly useful...
Showing: 401 - 450 of 929 results