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Showing: 401 - 450 of 1092 results

  1. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...maps. Resources on Addgene.org Sequence Analysis of your Addgene Plasmid Addgene Plasmid Guide     ...This post was updated on Dec 4, 2017. At Addgene, we continually use the Basic Local Alignment Search...databases, and elements of the BLAST search pages. At Addgene, we use blastn to identify any discrepancies in... viewing blastx results. When using blastx at Addgene, we use the default “Non-redundant protein sequences...NCBI BLAST website. Additional Resources on the Addgene Blog: Plasmids 101: How to Verify Your Plasmid...

  2. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...discuss the importance of actionability on the Addgene blog and how SITN short-form blog posts help readers...Additional Resources on the Addgene Blog Resume Writing for Non-academic Science Careers 10 Steps to a Perfect...

  3. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    ...was contributed by guest blogger, member of the Addgene Advisory Board, and Associate Director of the Genetic... that model. Both libraries are available from Addgene as pools provided in two sets, each with 4 gRNAs...individual plasmid formats A first for a library at Addgene, you can also order the individual lentiGuide gRNA...PubMed Central PMCID: PMC4744125. Resources on the Addgene Blog Learn to Conduct Genome-wide Screens with...CRISPR for Single Base Editing Resources on Addgene.org Browse Our New and Improved Pooled Library Page...

  4. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...the wide array of Cas9s (many available through Addgene’s repository) and make it easy to find your perfect...variety of species and cell types can be found on Addgene’s website. Activating target genes using dCas9-based...variety of species and cell types can be found on Addgene’s website. Fig. 3: Cas9 repressors include...What do i do next? Designing or selecting a sgRNA Addgene may carry a validated sgRNA for your target gene...Next CRISPR Experiment Cas9 expression systems Addgene carries a variety of Cas9 containing plasmids that... ). 368, 290–296 (2020). More resources on the Addgene blog Read all the posts in our CRISPR 101 Series...Library Experiments Additional resources on the Addgene website Find CRISPR Plasmids Catch up on your ...

  5. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...plasmids with Addgene. Additional control plasmids for the TKO library will be available at Addgene soon. Hart...cellular compartments (cytosol, Addgene catalog #71245; mitochondria, Addgene catalog #71247) Rosella provides...read? Listen to our hot plasmids segment on the Addgene Podcast. View Plasmids from:  March (Q1) | June...sequencing. The STARR-seq backbone is available from Addgene in both human and fly versions. Arnold, et al....alongside a growing number of CRISPR libraries from Addgene’s repository that can be used to perform screens...PMID: 24395366 Oxford Drosophila CRISPR library Addgene has had the privilege of distributing several human...promoter and Cas9 from the actin 5C promoter. Addgene’s fly library web page offers more details and protocols...

  6. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...Additional resources on the Addgene blog Read about company culture at Addgene See some of our previous ...costumes, and spooky science stories. Yes, please! At Addgene, we love finding ways to incorporate science themes...teams dressed up as plasmids, Blugene, and the Addgene ping pong table. This year, in addition to the ...Halloween is no exception. For the past 10+ years, Addgenies have formed teams, chosen a theme for the costume...

  7. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...to highlight some of the top articles from the Addgene blog this year. Spoiler alert: many of the top ...SARS-CoV-2, including many with plasmids deposited at Addgene! 2. DNA Purification Without a Kit by Beth Kenkel...

  8. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    ...community, so email us at blog@addgene.org if you are interested. Addgene's Most Popular Posts of 2014 ...year in science – as seen through the lens of Addgene’s blog and plasmid repository. Our blog is just ...

  9. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...plasmids you’d like to deposit with Addgene, be sure to visit addgene.org/deposit. Who knows, maybe yours ... articles cite the use of pMD2.G obtained from Addgene. Skimming the list, one gets a sense of the broad...searchable list here). Tips for using pMD2.G At Addgene, we use pMD2.G for producing our ready-to-use lentivirus...Lentiviral Transduction Additional Resources on Addgene.org Check out our Viral Service Learn more about...

  10. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    ...promoter in rat. Contribute your AAV data Just like Addgene’s plasmid repository, the AAV Data Hub is a community-driven...only accepting data for materials distributed by Addgene.  Future directions As the AAV Data Hub grows, ...accelerate your research. Sincerely, Melina & The Addgene Team ...

  11. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...Additional Resources on the Addgene Blog Read Additional Cancer Blog Posts Addgene's Cancer Collection Pages... between tumor and immune cells in real time. Addgene depositor Professor Max Krummel from UCSF showed...engineering (Ref 5)  as described by Blue Flame Addgene depositor Professor Michel Sadelain from the Memorial...poster is pTomo-Ras which has been deposited at Addgene was used to produce the glioblastoma model for ...with the scientific community, contact deposit@addgene.org to find out more about our free deposit and distribution...: Deciphering the Relationship Resources on Addgene.org Browse Cancer Plasmids Browse Immunology Plasmids...

  12. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    ...tools. Find the mouse CRISPR knockout library at Addgene. Henriksson et al., Cell, 2019. PubMed PMID: 30639098...cardiovascular disease. Find the LipoGlo plasmids at Addgene! Lawler et al, QJM: An International Journal of...systems. Find these CRISPR activation plasmids at Addgene. Liu et al., Nature Communications, 2019. PubMed...worms and have made these constructs available at Addgene. These include Arch(D95N) and QuasAr, as well as...biosensors at Addgene. Hashemi et al., PNAS. 2019, PubMed PMID 31371514. ... Various Addgenies...

  13. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ... planning an experiment to performing one.  At Addgene, over 120 labs have deposited CRISPR reagents, ...already been shown to work in CRISPR experiments. Addgene’s dedication to resource sharing has led us to develop...table columns are target gene, species, sequence, Addgene plasmid ID (if we have one associated with the ...Sharing. 2016. In press. Additional Resources on the Addgene Blog Learn How to Design Your gRNA Use the CRISPR...This post was written jointly by Addgenies Brook Pyhtila and Nicole Waxmonsky Resource sharing shortens...of Our CRISPR Posts Additional Resources on Addgene.org Browse gRNA Design Tools Read Our CRISPR Guide...

  14. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...University Medical Center are now available at Addgene along with a description of their experimental ...The three plasmids which have been deposited to Addgene are: pAdSh.PGK.Cas9, pAdSh.U6.gRNAS1, and pAdSh.U6...Maddalo et al., 2014). These tools are available at Addgene and could be useful to labs wishing to create this...including protocols, check out the plasmids at Addgene: pAdSh.PGK.Cas9 (expresses S. pyogenes Cas9 from...technology guides, FAQs, and CRISPR resources on Addgene's CRISPR page. References Beatty MS, Curiel DT ....1089/hum.2015.087 Additional resources on the Addgene blog How to Design Your gRNA for CRISPR Genome...

  15. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...adeno-associated viral (AAV) vectors available at Addgene are suitable for in vivo studies, our scientists...measuring viral titer and assessing purity. But Addgene is also conducting more rigorous quality control...plexWell technology, we can now perform NGS at Addgene to sequence all the DNA packaged inside the viral...AAV-68544, the best match for the contaminant is Addgene plasmid #44362. Method validation, limitations,...commonly performed for research-grade materials. Addgene is going the extra mile and pioneering the systematic... Press, pp 247–278 Additional resources on the Addgene blog Check out our Viral Vectors Featured Topic...discuss. We are also available by phone or at help@addgene.org to answer your questions about our quality control...

  16. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...yielding promising results in the laboratory, and Addgene is accelerating this research by helping to make...possible. Connecting the research community - Addgene's cancer collection pages Scientists are making...latest resources? In response to this challenge, Addgene has launched an initiative to curate our collection...direct the investigator to a list of all available Addgene plasmids he or she can use to study it (as seen...section below. Michael G. Lemieux is a Scientist at Addgene with interests in education and scientific writing...Institute: Ras Pathway v2.0 Page Resources on the Addgene Blog Tackling Cancers' Drug Resistance with a ...Used in Cancer Lineage Tracing Resources on Addgene.org Visit our Cancer Collection Pages Deposit Your...

  17. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    ...molecular weight ladders were recently deposited with Addgene. A team of undergraduate students led by Dr. Song...pPSU1 and the pPSU2 plasmids are available from Addgene. Learn How to Analyze a Restriction Digest References...Central PMCID: 5446395. Additional Resources on the Addgene Blog Are you a Frugal Scientist? Save Money with...the Evolution of Lab Techniques Resources on Addgene.org Use the Kit-Free Alkaline Lysis Plasmid Miniprep...

  19. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...sometimes unclear or missed altogether. Emailing Addgene might not be as high on the published-paper-celebration...in this status — a win-win situation for all. Addgene will release plasmids on hold if they are clearly...distribute unpublished materials. What about preprints? Addgene supports preprints and encourages authors to share... for reading, and for depositing plasmids with Addgene! We'll continue to review our policies to make ...celebrate!), send us a quick email at deposit@addgene.org with the citation and your deposit or plasmid...website, Help Center, or email us at deposit@addgene.org. ...

  20. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    ...talked a lot about the quality control process at Addgene by introducing our new sequencing partner seqWell...blog, we’ll walk you through how a scientist at Addgene uses Snapgene to confirm the sequence of a plasmid...designed many primers for Sanger sequencing at Addgene. By using Snapgene’s “Import primers from a list...sequences coming soon!  Additional Resources on the Addgene Blog How to verify your plasmid Check out our ...featured topic page Additioinal Resources on Addgene.org Molecular Biology Reference Pages Basic Molecular...

  21. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    ...thread ⬇️⬇️⬇️ — Addgene (@Addgene) April 15, 2020   Additional resources on the Addgene blog Read about...essential research into COVID-19 (like here at Addgene!), and new databases are supporting their access...guide for external collaborations Resources on Addgene.org Browse our COVID-19 plasmid collection ...

  22. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...resources at Addgene Check out Addgene’s plant vectors! Find the plasmids from this paper at Addgene. Other...as plasmid yield during preparation in E. coli. Addgene depositor Indu Maiti has created a new and versatile...

  23. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ... Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein Plasmids and Resources... GFP is great news for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used...PubMed. Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis...

  24. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    ...We’re happy to introduce the newest member on Addgene’s Board of Directors, Bart Newland. Newland is General...all. Q: How does your experience help you as an Addgene board member? BN: Having seen so much in the business.... I think these are things that I can bring to Addgene -- that comfort with saying, “if we do this, then...

  26. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...workshops and organizational skills. Meanwhile, Addgene was also thinking about ways to address irreproducibility... to Teytelman’s connection with Joanne Kamens, Addgene also had the opportunity to contribute to this ...anyone could present the material. For example, Addgene’s contributions by Tyler Ford included reagent sharing...Tsang for editing. Additional resources on the Addgene blog: Check out this guide on Transferable Skills...workshops Levelling Up: Reproducibility for Everyone Addgene: a global resource working to enhance scientific...

  27. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...editing LARRYv2 pooled libraries for cell barcoding Addgene’s own AAV6 and AAV11 packaging plasmids Mapping ...planning on lounging poolside or swimming laps, Addgene has pooled libraries for any summer project. While...  New AAV6 and AAV11 packaging plasmids from Addgene By Meghan Rego Attention AAV users: in an effort...effort to expand your gene delivery toolkit, Addgene now offers packaging plasmids for AAV6 and AAV11! Why...projection neurons. Created with BioRender.com. Addgene has tested these capsid plasmids in vitro and uses... viral vectors ready to use in your lab. Find Addgene’s AAV6 and AAV11 packaging plasmids here!   An ...

  28. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Antibodies Resources on addgene.org Addgene's Antibody Purification Protocol Addgene's Video Antibody Purification...of recombinant antibodies (rAbs) exist (though Addgene has a great collection of ready-to-use rAbs and... are contained in (Randolph et. al., 2018). At Addgene, we ensure the efficacy of our antibodies through...Resources and references More resources on the Addgene blog Introduction to Antibodies Plasmid-based Recombinant...Purification Protocol Addgene's Antibody Guide References Trimmer JS. Recombinant Antibodies in Basic Neuroscience...

  29. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...Resources on Addgene.org Learn how Addgene produces AAV and lentivirus Check out Addgene's virus protocols...needed to expand the HEK293 cells. Check out the Addgene AAV Production protocol for more details. Pro tips...because I don’t have enough AAV. Compared to the Addgene AAV Production protocol, I take a lazier approach... the qPCR run and data analysis. Refer to this Addgene AAV Titration by qPCR Protocol for more details...PubMed PMID: 18265393. Additional resources on the Addgene blog Find out what makes AAV a versatile tool ...

  30. Deck the Lab 2022!

    Type
    Blog Post
    ...Follow @Addgene, use the hashtag #DeckTheLab, and tag @Addgene in the post! In January, Addgenies will ...or Instagram with #DeckTheLab and tagging us, @addgene to be judged! In previous years, scientists have...

  31. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...Check out Addgene’s Step-By-Step Protocol Pressed For Time? Order Ready-to-Use AAV from Addgene ...PubMed PMID: 18265393. Additional Resources on the Addgene Blog Important Considerations When Using AAVs ...for Gene Expression in Mammals Resources on Addgene.org Find AAV Packaging and Transfer Plasmids Here...

  32. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    ... Sciences in Ghana will be receiving the third Addgene/Seeding Labs plasmid grant and plans to use the...future. Future awardees We will be opening the Addgene/Seeding Labs Plasmid Grant to a third round of ...inspiring research. Additional Resources on the Addgene Blog Learn How We Raised Money for Rare Disease...Grants to Rare Disease Research Resources on Addgene.org Check out our Cancer Pages Learn about Global...

  33. Editor's Choice, July 2016

    Type
    Blog Post
    ... them. July 2016 was a fantastic month for the Addgene blog. We achieved roughly 50,000 views over the... Science Communications Intern (and soon to be Addgene Scientist!) Mary Gearing, this post focuses on ...to read all of our excellent posts from July: Addgene at the ASM Microbe 2016 Conference Generating Mouse...

  34. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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    Blog Post
    ... and have the biggest impact. We should know — Addgene has built its foundation on this idea! And we believe...high specificity and brightness, now available at Addgene!   {% module_block module "widget_58e2d882-2fb6...experimental requirements. Best-in-class collection at Addgene The GTA contains data on thousands of AAV enhancer...enhancer plasmids, which are all available at Addgene. This allows scientists to choose enhancers that may... or target a very specific cell type. However, Addgene has partnered with the AIBS to showcase the “best-in-class...Science. There are also more resources available at Addgene, including the Allen Institute for Cell Science...doi.org/10.1002/bies.202300044 Resources on the Addgene blog Plasmids 101: Gene, enhancer, and promoter...

  35. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...protocols Sep-OTSλ plasmid - Addgene # 68292 C321.ΔA bacterial strain – Addgene #68306 pCRT7 NT TOPO E17TAG...E17TAG GFP plasmid - Addgene #68295 SupD plasmid – Addgene # 68307 For further technical reference and protocols...flavors’ of Sep-OTS plasmids are available through Addgene, but we have worked most extensively worked with... recombinant phosphoproteins’ available on the Addgene website. Many thanks to guest blogger Natalie ...Protoc. doi 10 (2008). Journal. Resources on the Addgene Blog Verify Your Constructs with Colony PCR Read... Tags for Protein Purification Resources on Addgene.org Find Synthetic Biology Plasmids Find Bacterial...

  36. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ...conference format. Additional resources on the Addgene blog Read all of our blog posts about conferences...scientific conferences have followed suit. Two Addgenies, Susanna Bachle and Yijun Zhang, recently attended...conferences (TAGC and ASGCT, respectively) and Addgenie Angela Abitua hosted a virtual Reproducibility...

  37. Donations from Addgene to Yield Answers for Rare Disease Researchers

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    Blog Post
    ...those rare diseases will receive plasmids from Addgene worth a total of $5000.  Lowe Syndrome One of those...the award from the Rare Genomics Institute and Addgene will make a substantial difference in pushing the...iPS) technology and CRISPR-Cas9 constructs from Addgene to gene-edit mutations into XP genes and other ...analyses.  “We believe that with this award from Addgene, we’ll be able to make big progress in elucidating...

  38. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    ... who each are being awarded free plasmids form Addgene to accelerate their research. Read on to learn ...how these researchers plan to use plasmids from Addgene to advance their research on diseases of glycosylation...this, Prof. Videira and collaborators will use Addgene plasmids to immortalize CDG patient-derived immune...and other specific gene sequences available from Addgene. Dr. McMurray hopes that by demonstrating GdnHCl...neural stem cells. The lab will use plasmids from Addgene to construct NFIX expression vectors as well as...

  39. Developing Lab Management Software for Biology

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    Blog Post
    ...placed on the Addgene website, it sets off a chain reaction of events throughout all of Addgene - within the... of thousands of plasmids a day, like we do at Addgene, pen and paper will fail you. The benefits of lab...fun too. Kris Shamloo is a Software Engineer at Addgene and is interested in writing software tools to ...krisshamloo.com     Additional Resources on the Addgene Blog Learn about How We Use Barcodes in the Lab...Track Your Experiments Additional Resources on Addgene.org Find Plasmids for Your Research ...

  40. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...At Addgene, we love GFP, and we’re always excited when depositors find new ways to make this workhorse...proteins. The T-DOGGs and Cre-DOG are available from Addgene, and we’re eager to see how you use them in your...PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “Cell type-specific...26258682. Find plasmids from this publication at Addgene. 3. Kirchhofer, A., et al. “Modulation of protein...Biol. 19 (2012), 117–121. PubMed PMID: 22157958. Addgene Resources Cre-lox Resource Guide Fluorescent Protein...

  41. Year of the Camelid: Antibody Style

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    Blog Post
    ...from addgene.org  Plasmids encoding nanobodies at Addgene Sybody Generation Toolbox from Addgene     ...to human populations around the world. Here at Addgene, we love camelids too, though not just for the ...10.1038/s41586-021-03676-z  More resources on the Addgene blog Introduction to Antibodies  Secondary Nanobody...

  42. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...-59259-301-1:99 Resources on Addgene.org Addgene antibodies Addgene’s antibody guide Antibody protocols...having solubility issues!). Need a GST antibody? Addgene has you covered! His His tags, often called polyhistidine...protocols  Resources on the Addgene blog Plasmids 101: Protein tags Antibodies 101: Epitope tags To Each...

  43. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ... More resources from Addgene The AAV Data Hub Addgene's Viral Services Addgene's Viral Protocols...10.1007/978-3-319-07758-1_2 More resources on the Addgene blog Viral Vectors 101: Viral Applications Viral...

  44. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...PMC5517107. Additional resources on the Addgene blog Subscribe to the Addgene blog Find lab tips for your research...to learn about practices I could bring both to Addgene, and share with my peers. In fact, there was so...resources by sharing plasmids Find plasmids at Addgene ... about conservation in the lab Resources on Addgene.org Sign up for our quarterly newsletter Help other...

  45. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    ...Internship with Addgene, I didn’t know a lot about scicomm, but had enjoyed writing a few Addgene guest blog...careers here Resources on Addgene.org Learn more about careers at Addgene ...Twitter @HansPacker. Additional Resources on the Addgene Blog Get tips on how to do your own informational...

  46. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...Additional Resources on Addgene.org Brush Up on Your CRISPR Background with Addgene’s CRISPR Guide Page Find...try, as always, the plasmids are available from Addgene. Happy CRISPRing! References Harrington, Lucas...bioRxiv (2017): 138867. Additional Resources on the Addgene Blog Read about CRISPR Methods for Bacterial Genome... Find CRISPR plasmids at Addgene ...

  47. Hot Plasmids - October 2022

    Type
    Blog Post
    ...available! by: Ashley Waldron The majority of Addgene's more than 50 antibodies target neuroscience-related...protein tags - a quick search for ‘Myc tag’ in the Addgene catalog pulled up over 13,000 plasmids! Our recombinant...love to hear from you!   Fig. 3: Image of Addgene’s western blot for the myc-tagged protein GFP-smFP-myc...Anti-c-Myc [9E10] page for more details. Image credit: Addgene.   Find anti-c-Myc antibody here!   Evan, G...

  48. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...below and an Addgenie will help you find what you need.   More Plasmid Resources from Addgene: Plasmids... is which vector will best suit your needs? At Addgene, we have a vast collection of empty backbones that... of the specific mutations. For these purposes Addgene has a large collection of yeast expression vectors...Plasmids 101 blog series Addgene's Molecular Cloning Guide & Other Plasmid Protocols List of common epitope ...

  50. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ...for all incoming plasmids was a big change for Addgene - we had used Sanger sequencing for quality control...provide requesting scientists high quality data! Addgene's new in-house NGS process To start, the process... the plasmid sequences). As with all things at Addgene, each part of this new process involves a significant...available as a partial sequence with the heading "Addgene Partial NGS Result." In some cases, there will ...
Showing: 401 - 450 of 1092 results