...Penguin Publishing Group. Day, F. (2019). Embrace your weird face your fears and unleash creativity. Gallery...focus. Find yourself a block of time and a quiet space where you can address the issue head on. Prepare...can come any time. Meetings shouldn’t be the only place people share ideas. Ideas had over lunch are just.... Identify your enemies In Felicia Day’s book Embrace your Weird, Day identifies nine enemies that stand...backordered for months, we have to have plans in place to be able to continue our experiments. Thinking...team has used these techniques to optimize our lab space, our protocols and even our Lab Inventory Management...

...the sequence is located just upstream of a Protospacer Adjacent Motif (PAM sequence). The 3-5 nucleotide...modifications at DNA targets adjacent to NGG PAM sequences when used in place of wild-type SpCas9. The remaining...enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target, but many do not... REPAIR (RNA Editing for Programmable A to I Replacement) system fuses Cas13b to RNA deaminase ADAR2 to...

... Angela DePace has put personal development at the center of her lab (pictured above). DePace holds yearly...through thoughtful planning and honest feedback DePace’s yearly planning meetings revolve around two simple...tone of the meeting is almost more important. As DePace says, science is full of “terrible perfectionists...have tangible benefits for both PI and trainee. DePace and her lab members have noticed that the meetings... Park J, Bragdon MD, Lopez Rivera F, Biette KM, DePace AH. Mol Cell. 2015 Jun 4;58(5):718-21. doi: 10.1016...

... in the extracellular domain which activates the native signaling cascade of the intracellular part of...ligands (PORTLs). OptoXRs are chimeras of the extracellular domain of endogenous light-sensitive receptors...receptors, like mammalian rhodopsin, and the intracellular domain of an endogenous GPCR, such as adrenergic...signaling pathways? Here’s where photoswitchable intracellular proteins come into play. Photoswitches can be...state.      Figure 4: Example of an intracellular signaling pathway activated by an optogenetic...., & Cui, B. (2015). Optogenetic control of intracellular signaling pathways. Trends in Biotechnology,...

...rich and diverse workforce. Out of all the career spaces I explored for my post-PhD life, the science policy...science policy. Luckily, there are many mechanisms in place to ensure your effective networking---and they’re...pandemic has foisted upon us. Action Items:  A simple place to look for science policy folks is on Twitter- ...! Someone from your alumni community is a great place to start. What is science policy? THE IMPOSSIBLE... policy is so diverse, you can do it in so many places-- not just the government. When you talk to folks...technologies accessible to patients. And what better place to do that than at the nation’s largest integrated...to chat with someone working in a science policy space you’re interested in. Many fellowship programs can...

... entered human cells via hACE2, could not enter cells expressing murine ACE2 (Subbarao et al., 2006). ...SARS-CoV-2. The resurgence in popularity of the hACE2 transgenic mouse strain, for example, is sustained...the University of Iowa (McCray et al., 2006), the hACE2 transgenic mice were designed after it was discovered...Repositories around the world facilitate the rapid pace of SARS-CoV-2 research by removing logistic hurdles...Look DC, Perlman S (2006) Lethal Infection of K18-hACE2 Mice Infected with Severe Acute Respiratory Syndrome...

... that helps attack extracellular pathogens.  Transportation of antibodies to places they can’t reach on...valency of 10 antigens. An extra constant domain replaces the hinge region in IgM antibodies. IgM has no.... IgD While IgD is coexpressed with IgM on the surface of most immature B cells, its function is unknown.... They are found bound to IgE receptors on the surface of basophils and mast cells, two types of white... the intervening DNA sequence is deleted, which places the variable region next to a different constant...

...target proteins. The surface-exposed loops of nanobodies seemed like a good place to put AsLOV2 because...inserted AsLOV2 into conserved sites in all eight surface-exposed loops of an mCherry nanobody. Only insertion...-dependent shift in mCherry fluorescent to the surface of the bead, suggesting that OptoNBs still bound...bound their target protein even when stuck to the surface of a bead.  Using OptoNBs to control cell signaling...With darkness, SOS binds Ras and initiates the extracellular signaling-regulated kinase (ERK) signaling cascade...

...’t have to worry about having enough space! There’s enough space here at our new location to grow. We ... welcoming, inclusive workplace for everyone. We had Unbiasing Training in place before it was cool or...supportive Board, we have a robust committee process in place and continue to incorporate additional Board best... hard but as a 3 year Boston Globe and BBJ Best Place to Work with a positive, supportive culture, we ...

...other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol, 0.5% peracetic acid, ...they are not currently provided. Eye protection or face shields are required whenever there’s a splash or... and removed in a biosafety cabinet. For ultracentrifugation, heat sealed tubes can be used for extra ...decontaminate after use. Virus-exposed plastics and other surfaces should be thoroughly decontaminated after use,...basically, you’ll need to get trained in each new place (and pay attention to the trainings!), even if you...

...flanked by randomized nucleotides in place of a protospacer adjacent motif (PAM). They're used to determine... GFP on the surface and “receiver” cells express an anti-GFP nanobody on the cell surface. When the cells...cross-reacts with BFP, sender cells expressing surface BFP generated BFP-labeled receiver cells. What’...

...But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists ...research, and prevent technological advances from exacerbating existing inequalities and injustices. The Personal...at pgEd, I take on a variety of projects in the space of science education, communication, and policy....quickly into sensitive topics and uncomfortable places, from sticky ethical dilemmas to dark episodes ...policy requires broad interest in issues at the interface of science and society, and an ability to talk...in the legal, ethical and policy issues at the interface of biology and society.     Additional resources...

...target cell line, researchers often find themselves faced with a roadblock. In this post, we will provide ...concentrated by a variety of methods such as ultracentrifugation, centrifugation on a sucrose gradient, spin...starting. We recommend calculating the ratio of the surface area of the larger vessel to the 10 cm2 dish and...some final adjustments, this is generally a good place to start. If you don’t anticipate that you will ...relatively inexpensive and does not require an ultracentrifuge, PEG will precipitate all proteins in a solution...high-titer lentivirus preparations without ultracentrifugation. Sci Rep. 2015; 5:13875. PubMed PMID: 26348152...

... by ultracentrifugation (75,000 - 225,000 g for 1.5 – 4 hours at 4 °C). After ultracentrifugation, virus...vectors) you can concentrate your virus by ultracentrifugation of the viral stock, followed by resuspension...packaging cell debris before proceeding to ultracentrifugation to avoid contaminating your transduction ... to concentrate a virus solution without ultracentrifugation, you can reduce the volume of cell culture...exchanging the medium after transfecting cells, replace the medium with half the volume typically used ...

... Specially constructed plasmids can be used to replace genes in yeast, mice, or Drosophila through homologous...targeting takes advantage of this natural process to replace a targeted genetic locus with homologous sequence...gene will be removed from the chromosome and and replaced by the resistance gene.The gene is thus disrupted...recombination, the GFP/NeoR cassette is inserted in place of exon 2. Thus, the targeted gene is disrupted ...normally because one of its exons has simply been replaced with the same sequence flanked by LoxP sites while... while the resistance cassette has been placed into an intron.    Figure 4: A targeting vector...

... they not only traced the 3-D path of the protein’s backbone in three-dimensional space, but their flexible...ribosomal subunit, he kept sliding his palm across the surface of the protein where the nascent polypeptide emerges...realized how perfectly flat the complex is on this surface”….where the complex is docked on the membrane of...serious way. We represented the Cas9 protein in a surface format made of rock-hard, inflexible plaster. As...primed for cleavage. This structure captures the displaced non-target DNA strand and shows the HNH nuclease...

...student at the Max Planck Institute of Colloids and Interfaces. Here are some tips for cell culture that will...Clutter not only makes it difficult to clean your workspace properly, but can also disrupt the laminar air...materials into the hood. This will give you enough space to move around freely without causing spills. Also... than 80-85% confluent because cells like their space. If they reach confluence, they stop growing (contact...cells stored in their original condition and can replace your working stock if it gets too old or contaminated...lab at the Max Planck Institute of Colloids and Interfaces. Sana is particularly interested in Glycobiology...

...of common genetically encoded neuronal tracers Neuronal tracers such as epitope tags and fluorescent proteins...Spaghetti Monster - A new and improved neuronal tracer With the limitations of epitope tags and fluorescent...fluorescent proteins, it has been crucial to develop new tracers that enable accurate labeling of neurons. As discussed...Addgene Uses of smFPs 1. Multi-channel connectomic tracers Fluorescent proteins such as GFP have been used...labeling experiment using smFPs and standard neuronal tracers. 2. Visualization of neuronal sub-cellular structures...

...in-house software has been crucial for us to keep pace with our current need to analyze nearly 300 sequences...sequencing data collection and analysis eventually outpaced our software, even with continuous updates. The...other bioinformatics purposes  Integrated user interface, so our QC team could initiate and monitor the...open-source sequence assembly tools at the appropriate place in our workflow without having to translate or convert...Biopython.  We started at the usual recommended place: documentation. We oriented ourselves with the basic...smoothly operate the pipeline, we needed a user interface. In our next post, we’ll share our story about...

...simply embellish and adorn your lab space or your work from home space in a fun, festive holiday or winter...winter or holiday theme decor in your work from home space… but with a science twist. Maybe you have a collection...using life science catalog pages. Make your WFH space feel a little more festive this holiday season! ...Instagram as we share pictures of our decked out spaces.       ...