A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
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Blog Post
...one of lox2272 sites will now be sandwiched between 2 loxp sites and is excised by Cre (Figure 1). This ...flank the transgenes (non-homologous pairs) (Figure 2.). They found that almost all off-target expression...exercise caution when using them.
Figure 2. Schematic diagram showing the mechanism of spontaneous... Tropism and Axonal Transport of AAV Serotypes 1, 2, 5, 6, 8 and 9 in the Mouse Brain. PLoS ONE 8:e76310... Pseudotyped with Viral Capsids from Serotypes 1, 2, and 5 Display Differential Efficiency and Cell Tropism...and volumes to determine the optimal injection parameters needed to transduce your cells of interest. Using...Once you have determined the optimal injection parameters for the serotype that best transduces your cells...