...immunogenic properties of the final product, particularly in large animal studies. Endotoxin contamination...detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). pAAV-CAG...being discarded and replaced with a new vial of early passage cells. Approximately two weeks post-thaw...

...could produce ultrasound contrast. That is, until earlier members of our lab - the Shapiro Lab - developed...applicability. They also did not scatter ultrasound nonlinearly, making them difficult to distinguish from background...next-generation bARGs that could scatter ultrasound nonlinearly and be easily expressed heterologously by bacteria...

... cell types depending on their serotype, are particularly challenging. Their efficacy in any given system...from 2016–2023. All three generations are regularly requested and a valuable part of our viral vector..., new partnerships, and new platforms, from our early engagement with UPenn to our launch of the redesigned...

...out.  In some fields, a cover letter is vital, particularly in fields where communication and writing are...skills-based ones, may not use them very often. Similarly, entry-level jobs are less likely to require cover...working for that organization or group. This is particularly important for labs and nonprofits, who tend ...

...products may be toxic to the host when expressed, particularly those that are insoluble, act on DNA, or are... can neither restrict nor methylate DNA. dcm: Similarly, strains with this mutation are unable to methylate...dead cells, and, conversely, you cannot induce too early as there are not enough cells in the culture to ...

...inconvenient data must not be ignored. Returning to an earlier analogy, no amount of shouting will make an apple...comparison to 37% of the general public (5, 6). Similarly 90% of active research scientists believe climate...13th the March for Science homepage has amassed nearly 500,000 ‘likes’, while their website lists over...

...transparent. Thus scientists can easily observe early developmental stages which can be difficult in other... large experiments over multiple generations. Similarly to other model organisms, the C.elegans genome... PubMed PMID: 26712014. Pandey, Udai Bhan, and Charles D. Nichols. "Human disease models in Drosophila...

...fluorescent and can be challenging to track intracellularly, but aptamers permit the study of intracellular...is better able to maintain its fluorescence intracellularly due to a lower dependency on magnesium and ...binding with RNA by about 7x). Mango fluoresces nearly as brightly as Spinach, but binds with much higher...

...research directions that were inaccessible with earlier tools. They also produce more than 20 other MTRIA...MTRIA biosensors are generated by inserting a circularly-permuted GFP into an intracellular loop of various...brightness, expression, and sensitivity compared with earlier SF-iGluSnFR. Image from Aggarwal 2022.   Find iGluSnFR3...

...researchers investigating HIV integrase mutants in the early 1990s (Leavitt et al., 1996), and since then, numerous...severe combined immunodeficiency (SCID-X1) in the early 2000s. These trials unfortunately resulted in several...transduction.  The process for producing your own IDLVs is nearly identical to that of regular lentiviral vectors...

...improve muscle function, and they must be injected regularly. Dystrophin and genome editing Since complex oligonucleotide...approaches for exon skipping. Addgene depositor Charles Gersbach used paired zinc finger nucleases to remove...force, and other muscle tests, muscle function is clearly improved, although not to wild-type levels. With...

...the description to watch any sections you’re particularly interested in, but I’d recommend watching the...Reproducibility Project: Cancer Biology have produced similarly low rates of reproducibility. Failed attempts ...modified analyses on published data if you need to. Similarly, FigShare also makes it easier to share data through...

...mouse model generation simpler, as described in an earlier post on generating CRISPR mouse models. For simple...the example at right using blue flame plasmid pmScarlet_C1! After creating the T7-DNA template, you'd use...right homology arm to the desired insert (e.g., mScarlet).   Plasmid ID Plasmid Name Tags ...

...are always traveling, or too busy to interact regularly with the newer lab members. You must get an idea... the lab head? Are meetings with the lab head regularly scheduled or only ad hoc? Does the mentor keep...at your data and suggest follow up experiments regularly? Can you work on some experiments of your own ...

...H3K27ac histone modification at specified loci. Charles Gersbach’s lab has deposited mammalian expression...pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1. LSD1 is a particularly large protein, and this fusion allows for packaging...this article. References Haldeman JM, Conway AE, Arlotto ME, et al (2018) Creation of versatile cloning ...

...) outside of the homology elements allowed for scarless insertion — no UTR sequence added to the genome...outside of the homology arms (red) to allow for scarless insertion of the payload (teal). The Cas9 gRNA...homology-directed repair (HDR), and insertion is scarless, unlike previous integrase-based approaches. Find...

...Controlling the expression levels of your gene may be particularly useful when the gene being expressed has threshold...transposase/transposon gene modification system and the scarless, Footprint-Free™ removal of cargo with the excision-only...accessory transposon sequences.  The use of PBx is particularly important to avoid any possible random re-integration...

...and applied research since their first use in the early 90’s to genetically modify primary cells. Amongst...un-transfectable cells, labs use viral vectors and particularly retroviral vectors because they target a broader...was obvious that its safety was a disadvantage particularly for clinical development. In addition, MoMLV...

...base-pair size nearly indistinguishable from a Watson-Crick base pair, are joined with nearly the same efficiency...high-throughput profiling of ligase fidelity that extends earlier studies through a high-sensitivity multiplexed ...

...reason most people enter postdoctoral training. Similarly, there are several factors to consider when selecting... get an experimental system up and running very early on. Once your system is working, you should maximize...to strengthen your new model?  PFs need to be regularly thinking about manuscripts. The other items will...