Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 381 results for: BRE

Showing: 161 - 180 of 381 results

  1. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...resistance gene you select? In this blog, we’ll break down the mechanism of action of the most common ...death by intercalating into DNA and generating DNA breaks. The Sh ble gene provides zeocin resistance by ...

  2. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ... current research. Understanding the depth and breadth of this bias, and then using that understanding...cancer cell lines (left). Heatmap indicating the breakdown of ancestry associated dependencies across ancestry...

  4. CRISPR Antimicrobials

    Type
    Blog Post
    ...restaurant chains including Chipotle and Panera Bread have pledged to eliminate the use of antibiotics... machinery, so a CRISPR-induced double-stranded break (DSB) in the genome is lethal. If this DSB occurs...

  6. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...group used an approach called BLESS (direct in situ breaks labeling, enrichment on streptavidin and next-generation...overcome with split AAVs. In general split AAVs break a large transgene in two pieces and package each...

  7. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...single-stranded nicks instead of the double-stranded breaks used in other insertion systems. Off-target nicks...nicks are much less harmful than off-target breaks, almost always resolved without any toxic effects to...

  8. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...centrifugation between washes which can cause loss of beads, break weak protein interactions, and limit high throughput...buffer has an optimal pH and ionic strength that breaks weak interactions from residual non-specific proteins...

  10. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...on studies in many disciplines has been a long brewing frustration for me. I read a variety of psychology... missing out on. Yet I believe that the big breakthroughs in my discipline, education, will come from ...

  11. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...variety of topics. Individual forums are hosted on subreddits (r/”topic”) which are essentially lists of forums...engaged, and has great questions! 2. The r/Science subreddit is well moderated. One of our original worries...

  13. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...gRNA Figure 1: Validation of double strand break (DSB) mediated homology dependent repair by enhanced...better that gRNA is at causing a double stranded break at the target site, allowing the two halves of EGFP...

  15. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...frameshift mutations through aberrant double strand break repair, it has proven more challenging to develop...editing via the introduction of a double strand break by using a targeted nuclease. Asuri et al, for example...

  17. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ... (HDR) of a CRISPR/Cas9 induced double-stranded break. This site-directed genome editing requires a template...researcher at Cornell University.       References 1. Breslauer, Kenneth J., et al. "Predicting DNA duplex stability...

  18. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...to specific loci without inducing double strand breaks. Below you'll find some of the CRISPR-based epigenetic...programmable and targeted de novo DNA methylation in breast cancer. Oncogene 34:5427–5435 . https://doi.org...

  19. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...Part 1: Genetically Engineered Mice and Part 2: Breeding and Crossing Mice. My first CRISPR mouse experiment...endonuclease that creates the DNA double-strand break (DSB) at the target site, and a donor oligonucleotide...

  20. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...standard DNA plasmid transfection, a double-stranded break may occur in your cargo. Additionally, with piggyBac...as CRISPR/Cas9) to create a double-stranded DNA break. After DNA cleavage, the piggyBac™ donor is used...
Showing: 161 - 180 of 381 results