We narrowed to 799 results for: Clu

...technology can be used for numerous applications, including transgenesis, gene-trap screens, and gene editing...manipulations in animal models or cells lines, including stem cells, without leaving behind any accessory...piggyBac™ transposon containing a selection marker is included in a standard homology directed repair template... used for a variety of research applications, including making transgenic cell lines and genetically-modified... using a variety of gene editing technologies including Zinc Fingers, TALENs, and CRISPR-Cas9 nucleases...

...high as 1-3%. In light of this finding, we now include guidance on the plasmid page of DIO/FLEx AAV vectors...you intend to use performs in your system. This includes a thorough testing of both the serotype and promoter... is reached you may obtain a false negative or conclude the viral vector is not working.  When using a...off-target expression that can confound your results.  Conclusion Using AAV vectors in your experiments requires...system. It is very rare for a methods section to include all of the variables that need to be accounted ...

...Wilson and Doudna, 2013). RNAi consists of two non-exclusive gene silencing pathways: transcriptional and post-transcriptional...afflicted by pervasive off-target effects. This conclusion was based upon numerous phenotype discrepancies...importance of using orthogonal methods to confidently conclude the function of a gene of interest. Tools for ...discussed below). Examples of these constructs include pGAPi, which allows easy creation of long hpRNA...experiment are now available on Addgene. These include the construct that allows for easy insertion of...

...various sources that are difficult to trace. These include the laboratory personnel, the serum, the cell culture...cross-contamination and due to poor lab techniques. These include reusing pipettes for multiple cell lines rather...Contamination! Tissue culture media often have indicators included in the media that turns yellow from lowered pH...the mycoplasma DNA appears in the form of small clusters, apart from the cellular DNA. Fluorescent DNA ... periods and affect the total cell viability. Conclusion To sum up, it is always important to prevent ...

... advice for the group of juniors and seniors, including that it’s never too early to start networking!...was the favorite part of their visit for many, including Natalie Reid who said “Seeing the bacteria on ... of programming for scientists!). Careers that include both science and business or programming were new...showed some highlights of his travels from 2015, including a great European photo slideshow from our UK team...

... impact downstream outcomes of an experiment, including the accuracy and efficacy of findings. If you’...peptides and antibodies. Example applications of this include coupling to an antibody, whereby excitation of ...them under experimental conditions. Common issues include photobleaching, non-specific binding, and quenching...biotechnology. Recent examples of advancements include the Janelia Fluor(R) (JF) dyes developed by the...super-resolution applications. Other current advancements include work exploring far-red-emitting dyes in bacterial...

...2015). This kit offers a broad range of parts, including copy number machinery, chromosomal integration...YTK and adds several additional capabilities, including conditional gene expression, multiplexed CRISPR...chromosomal integration and gene expression, the system includes a toolkit to express recombinant proteins within...kit plus the plant-specific parts you‘ll need, including sub-cellular localization signals, plant markers...engineering. This is a specialized system with parts including site-specific recombinases, regulatory elements...

...selection screening. They and their colleagues including Feng Zhang used the GeCKO library to identify ...libraries developed by MIT's Tim Wang and colleagues, including David Sabatini and Eric Lander, comprise 73,171...different gene targets. These pooled libraries include enrichment against kinases, cell cycle proteins...designed to target a smaller set of genes and it includes more guides per gene. The Sabatini/Lander library...

...building blocks including 14 different fluorescent proteins, 8 constitutive promoters, 2 includible promoters...Characteristics of the pHlash reporter protein include: H+ specific response cytoplasmic retention insensitivity...been used for a variety of experimental purposes including (1) single or multiple, constitutive or inducible...MXS-chaining were used in a variety of experiments including subcellular visualization of 6 different organelles...introduction of many different functional groups, including reactive groups for conjugation reactions, fluorescent... characteristics favorable for innocuous tags including high solubility, limited affinity for other proteins...mammalian cells with Broccoli Vegetable tags, including Spinach, Spinach2, and Broccoli, can benefit your...

... lobes, REC and NUC, and their subdomains. NUC includes the HNH and RuvC catalytic domains, as well as...as the CTD (also known as the PI), while REC includes most of the bridge helix. Created with BioRender.com...the cleavage domains, the recognition domains—including the CTD and the entire REC lobe—do not structurally...lobes. The guide RNA in this structure does not include stem loop 3, and guide RNA nucleotides 11-20 are...discoveries on this topic are still being made! Conclusion Finally, we can summarize the process from start...

...stays on the same page. Ideally, these meetings include all collaborators, from the lab members who are...like Zoom or Google Hangouts is a great way to include researchers from different locations. Having virtual...to your collaborators. This helps everyone feel included in the project and accelerates the pace at which...and morale. Especially when your collaboration includes scientists working in different time zones, it...

... and copy number of the plasmid) and typically include a gene that is advantageous for survival, such ...range of applications. Some common plasmid types include cloning plasmids, expression plasmids, gene knock-down...the many things that plasmids can be used to do include: Produce large amounts of a protein so that scientists...the lab. Addgene’s Molecular Biology Reference includes information about molecular cloning, how to choose...

... Now you’re wondering if your favorite gene is included in other CRISPR screens and whether that data ... each screen is captured by BioGRID curators, including the cell line/tissue type, enzyme system, sgRNA...-depth walk through of how to use ORCS:     Include your CRISPR screen data in ORCS  Researchers who...who wish to have their CRISPR screen data included in the BioGRID ORCS CRISPR database are encouraged ...

...replication, and fluorescent proteins that are already included in Snapgene’s extensive database. Snapgene also...of open reading frames, which is often our first clue about where the gene of interest might be located...plasmids are difficult to sequence by NGS. These can include GC-rich and repetitive regions. These regions require... same features annotated in our plasmid maps, including our most popular primers and certain restriction...

... collection of sgRNAs, but they may or may not include CRISPR machinery (which can be supplied separately...CRISPR pooled libraries! Addgene’s collection also includes a base editing sensor library, a base editing ...this library of all human transcription factors, including splice isoforms, to allow comprehensive screening...conducted with yeast. Created with BioRender.com. Conclusion Have we whetted your appetite for pooled libraries...

...distributing lentivirus (with many CRISPR tools included among the preps that are currently available) ... AAV preparations have worked well in my lab, including in applications where we have delivered them in...The blog provides tips for working with viruses including advice on how to titer lentiviral preps, troubleshoot...on each viral prep’s specific material page and includes western blots as well as other expression data...

... browse our entire AAV inventory. Our new AAVs include: Optogenetics AAV Control AAV Biosensor AAV And...H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both...have expanded our calcium sensor collection to include our first Flp-dependent GCaMP, AAV8-105714. Several... for the calcium sensor jGCaMP7 from Janelia, including three jGCaMP7 plasmids now packaged in AAVrg. ...

...the ins and outs of this classification system, including its history, biology, and usages in the lab. History...peptidoglycan membrane. Common gram positive bacteria include staphylococci and streptococci. Gram negative bacteria... lipid membrane. Common gram negative bacteria include salmonella and E. coli. Because of their thicker... For more specific procedural instructions, including timing, preparing the smear, and mixing the stains...

...span four continents and research diverse topics including microbiology, brain injury, drug discovery, and...PZA-resistance by using different approaches, including CRISPR and recombination-mediated genetic engineering...studied various aspects extreme life on earth including extremophile microorganisms, biomarkers, and recently...fluorescent signals from the sensor, and send a signal including its position in the river back to the user.   ...

...biological processes is highly useful in other fields, including synthetic biology. Addgene depositors Christopher...effectors are suboptimal for a number of reasons, including their potential toxicity and cross-reactivity ...level of “output” signal, was only about 10-fold, precluding the study of gene expression at very high or ...they can be used with many types of proteins, including Cas9 and dCas9-transcription factors, for which...