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  2. Common Injection Routes in Mice

    Type
    Blog Post
    ...delivery is also common. Due to the intubation, this procedure is considered quite invasive. Restraint: anesthesia...similar to its intratracheal counterpart, as both procedures target the lungs. The main differences are that...

  3. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...control of biological systems (Wheeler et al., 2016; Duret et al., 2019). But the field has sometimes seen ...plasmid here!   References and resources References Duret, G., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas...

  4. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...Mathis et al., 2024) and concurrent research by the Shendure lab (Li et al., 2024) have highlighted how chromatin... Lalanne, J.-B., Nathans, J. F., Lee, D. S., & Shendure, J. (2024). Chromatin context-dependent regulation...

  5. Pooled Library Amplifications

    Type
    Blog Post
    ... library is maintained and reduce recombination during the amplification process. In liquid culture, all...meant for repetitive sequences (such as STBL4 or Endura Duo), growing your bacteria on solid media, reducing...

  6. Experimenting with New Careers while in Grad School

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    Blog Post
    ...before founding CINSO. Now, the network I built during graduate school is my informal advisory board. ...to share valuable life lessons they have learned during their scientific training and wrote about them ...

  7. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...significant advances in tissue clearing techniques during this century.  The first successful attempt to ...215. Pubmed. W. Spalteholz. (1914) Uber das Durchsichtigmachen von menschlichen und tierischen Praparaten...

  8. DNA Purification Without a Kit

    Type
    Blog Post
    ...high yields and without degradation during the extraction procedure. Additionally, purified DNA was free...

  9. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...occurred once a week for several months at a time during the spring and fall semesters. Our scheduling was...Addgene bioinformatics journal club! What happens during journal club? There are many common ways to run...

  10. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...steps and outlined the considerations to be made during each step. Now that you know the basic principles...harsh - lipids and soluble proteins can be lost during fixation with these chemicals, which impacts sample...

  13. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ... of the sample name. If there was a minor error during processing, the window shows a yellow checked circle...Value - When the ICE linear regression is computed during generation of the ICE Score, the Pearson correlation...

  15. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...Planning Resources. The key to ensuring success during graduate school and beyond is to live in your lab...on what I have found to be especially beneficial during my own graduate career – and in fact, some of the...

  16. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...were kept at the appropriate temperature for the duration of their journey. Figure 2: Annual distribution...in their experiments, to enable virtual learning during the pandemic. Feedback like that lets us know that...

  17. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...terminal repeats (LTR). The 3’ LTR is duplicated during lentiviral integration, producing a second copy...voltage fluctuations, while having high fidelity during action potentials of fast-spiking neurons. The ...

  18. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...target cells Labs tend to follow the same general procedure for lentiviral infections, produce the virus, ...receptors, other important binding events take place during the course of infection. These binding events can...

  19. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...constraints given. And there were many constraints during the COVID-19 pandemic. The Pandemic Pivot Back...projects lined up, some that started production during the middle of lockdown, and some that will spin...

  20. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...stabilizes the extended open integrin conformation. During embryonic development, extended-open integrins ...development, and the shaping of the extracellular matrix. During immune responses, this same conformation encourages...
Showing: 161 - 180 of 441 results