We narrowed to 655 results for: EPO

...experiments in planarians with their recent plasmid deposit. These plasmids enable two main types of experiments...expression of specific gene expression. The 49 deposited plasmids enable students to study gene expression... genes (all of which are available through the deposit) are described in the Sánchez lab publication [...different planarian genes, we encourage you to deposit them back with Addgene and expand the number of...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here.     Listen to the Hot Plasmids...future work with this goal, the Barrick lab has deposited the pDCAF3-IS5 plasmid containing a synthetic ...

... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  Here's what...2024 under a CC-BY 4.0 license.  The team deposited an array of constructs for tagging the kinesins...Hao and Lee et al., 2024. The authors have deposited AAV plasmids for Cre-dependent and -independent...6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...

...Fluorescent Protein (GFP/EGFP) for a transcriptional reporter or as a general cytoplasmic label of cells, there's...FP dimerization. Equally problematic, several reportedly monomeric FPs are not actually monomeric, at ...Incorporation into a FRET biosensor A FRET biosensor is a reporter that undergoes a conformational change to bring...help you make better fluorescent fusion protein reporters for your studies and greatly lower your chances...

...expression plasmid. Experimental use Since the initial report describing CRISPR-mediated purification of a specific...including Protein A, 1xFLAG, 2xAM, and HA have been reported, as well as affinity purification with an anti-Cas9...chromatin immunoprecipitation (enChIP)." Scientific reports 3 (2013): 3171. PubMed PMID: 24201379. PubMed Central...Find CRISPR plasmids by function Find recently deposited plasmids ...

...are removed and replaced by a single (non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked...necessary to direct minigenome replication and reporter gene transcription by the EBOV polymerase (reviewed...the minigenome as a template to transcribe the reporter gene (e.g. firefly luciferase). The polymerase...start signal and initiates transcription of the reporter gene. The polymerase will elongate the mRNA until...

...technology more user-friendly and precise. We will deposit the plasmids of these systems to Addgene in the...Scientific Community Addgene: In 2012 your lab first deposited plasmids with Addgene. In 2014, you joined Addgene's..., as well as the Editorial Board of Scientific Reports and PLoS One. Additionally, you are involved in...shopping for a wide range of plasmids. The merit of depositing plasmids to Addgene has been spectacular. We'...

...maintaining fluorescence. The protein structure, first reported in 1996, is an eleven β-sheet-containing “barrel...constructing fluorescent fusions. Transcription Reporter: Placing GFP under the control of a promoter of...a given cell type. This type of transcription reporting was among the earliest uses of GFP. Fluorescence...micrometastases in nude mice in cancer studies, act as a reporter for DNA double strand break repair, and to label...

... extract as much information as possible from depositing laboratories and associated publications, but...would ‘spot-check’ critical regions of plasmids deposited with us via Sanger sequencing, while more recently...Generation Sequencing (NGS) technology. As our repository continues to expand, we realized the need to ...scale our lab work as we grew. As the number of deposited plasmids increased, the accompanying sequencing...

...pooled library.  Pooled libraries Scientists have deposited many types of pooled libraries here at Addgene... from the original library constructed by the depositing lab. (For our more popular libraries, you might...recommend following the protocols provided by the depositing lab whenever available. Addgene also shares the... other information or scripts provided by the depositing lab for data analysis.  I'm often asked whether...

...-founded by Harvard Investigators and Addgene depositors David Liu, PhD, Keith Joung, PhD, and Feng Zhang...Center will continue to expand as Rett scientists deposit their MECP2-related plasmid tools with Addgene ...Researchers can also find links to cell line repositories including patient-derived iPSCs, MECP2 animal...Neurological Disorder by Programmable RNA Editing. Cell Reports 32:107878 . https://doi.org/10.1016/j.celrep.2020.107878...

...content_id":null,"href":"https://www.addgene.org/depositor-collections/allen-institute-aav-enhancers/","href_with_scheme..."href_with_scheme":"https://www.addgene.org/depositor-collections/allen-institute-aav-enhancers/","type...evaluate the resulting expression of the fluorescent reporter, the brain tissue was extracted, sectioned, and...expression from mouse to primate neocortex. Cell Reports, 34(13), 108754. https://doi.org/10.1016/j.celrep...

...integrin antibodies available through Addgene’s repository. The collection includes 26 unique antibodies...in competition with other similar integrins, as reported by IPI and the Springer lab (Hao et al., 2024)...In this study, we, along with the Springer lab, reported the binding specificity, kinetics, and affinity... by IPI. Our integrin antibodies are the first deposit from an expanding collection of readily accessible...

... (Specific ​H​igh ​s​ensitivity ​E​nzymatic ​R​eporter un​LOCK​ing) technique Sensitivity: Single molecule...DNA polymerase, recombinase, and the ssDNA-FQ reporters that exhibit fluorescence upon cleavage. Upon ... results in the trans cleavage of the ssDNA-FQ reporter resulting in fluorescence. Cas12a binds all the...results in the continuous cleavage of the ssDNA-FQ reporter. The resulting fluorescence can be observed under...

...in the same lane, and the resulting fraction is reported. Figure 2: Normalization with loading controls...equally strong, so the normalized value simply reports which POI band is stronger. The right gel has exactly...abundance on your membrane, which would be directly reported by band intensity. Researchers use later steps...perturbed by the experiment Unfortunately, multiple reports suggest that common housekeeping proteins are influenced...

...of interest and co-expressing a cytosolic ZtSH2 reporter, they observe fluorescence localizes to the plasma...And orthogonal sensors with different ITAM/SH2 reporter pairs allow multiplexed measurement of different...10.1101/2022.08.13.503850.  iGECInano NIR calcium reporter Near-infrared (NIR) fluorescent proteins promise...photochemical characteristics than previous NIR calcium reporters iGECI and NIR-GECO2G, and its small size (~1500...

...that uniquely express selectable and/or sortable reporters in certain states of interest. For example, in...above (Sanger sequencing, qPCR, and fluorescent reporter-based) each come with their advantages and disadvantages...the most convenient strategy is the fluorescent reporter based method since it lends itself to cheap, quick...detection with flow cytometry. However, expressing reporters will consume cellular resources and therefore ...

...stage-specific promoter and design a UAS reporter construct, the reporter will only be expressed where and/or...UAS to examine the expression of reporter genes, or to combine reporter expression from one promoter with...

...approaches may become even more powerful following a report that one can use linear repair templates (ie. PCR-derived... 2014). A further development of this approach reported in 2017 uses the direct injection of a CRISPR/...templates (Farboud et al., 2019).   The Mello lab also reported that modifying the 5’ modification of oligos used...mixtures (Doksin et al., 2018). The Mello lab also reported efficient editing using a co-injection marker ...

...protein analysis products. You can find plasmids deposited by Promega here.    References and resources ...ligands for dopamine D2/D3 receptors. Scientific Reports, 10(1), 21842. https://doi.org/10.1038/s41598-020...fluorescence-based ligand-binding assay. Scientific Reports, 6, 25769. https://doi.org/10.1038/srep25769  Grimm...mitochondrial dynamics in mammalian cells. Biophysics Reports, 3(4–6), 64–72. https://doi.org/10.1007/s41048-...