We narrowed to 478 results for: FLU

...separate DNA or transforming cells with green fluorescent protein, these activities are limited to those...Typically, if you want to make a protein – say green fluorescent protein – you first need the DNA sequence that... instructions it needs to produce that green fluorescent protein through transcription and translation...students can easily sense, whether that’s bright fluorescent proteins that they can see, enzymes that can ...Stark, Jessica C., et al. "BioBits™ Bright: A fluorescent synthetic biology education kit." Science Advances...

...various structured surfaces. Plasmids expressing fluorescent proteins will be used to tag individual strains...within the biofilms will then be monitored using fluorescence microscopy and FACS. In an additional approach...this condition is crucial. Plasmids expressing fluorescent proteins and a new two-photon laser scanning ...plasmids containing promoter regions that activate fluorescent protein transcription in response to river contaminants...water samples at specific locations, measure fluorescent signals from the sensor, and send a signal including...

...microenvironment enzymes and metabolic reprograming influence tumors development? Being pathological active ...to cure cancer. To understand how cancer cells influence microenvironments and vice versa, let’s take a... being shaped by them. Another set of highly influential microenvironment cells are Cancer-Associated ...fact, Sanford H. Barsky and his team tested the influence myoepithelial cells had on the conversion of non-malignant...begun to understand two main areas of study that influence tumor microenvironments: immunology and angiogenesis...

...implications for the fusion protein effectivity), and a fluorescent protein tag for visualization (Fig. 1b). While...catalytically inactive dCas9) to modify chromatin. A fluorescent protein can still be included for visualization....   G-baToN can also be applied to other fluorophore/antibody pairs. Because the anti-GFP nanobody...quantify protein degradation the Ward lab used fluorescent proteins (FPs) fused to the AID* (minimal 44 ...Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair pathways, Amit Choudhary's lab developed...

...colleagues identified a highly bright and stable fluorescent protein, StayGold. Although it quickly saw wide...dimerization while maintaining the bright and stable fluorescence of the original (Ivorra-Molla et al., 2023)....Ivorra-Molla, E., et al. (2023). A monomeric StayGold fluorescent protein. Nature Biotechnology, 1–4. https://doi.org..., et al. (2023). Bright and stable monomeric fluorescent proteins derived from StayGold. ResearchSquare...mature marmosets. CAP-Mac also robustly expressed fluorescent reporters in green monkeys and cultured human...

...bacterial expression system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid collection...Kits for Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic biology... Enterococcus sp. Geobacillus sp. Haemophilus influenzae Helicobacter pylori Listeria monocytogenes Mycobacterium...Plasmids for Viruses Species Dengue Hepatitis Influenza Mumps virus Rubeola (measles) West Nile virus ...systems) and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression...expression - Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning...

... (Alias: CN4496) Expression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in... (Alias: CN2610) Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny... (Alias: CN2408) Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl... (Alias: CN2787) Expression of SYFP2 (yellow fluorescent protein) in striatal Pvalb+ neurons. Note strong... (Alias: CN3038) Expression of SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic...BGHpA (Alias: CN2700) Expression of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium ...BGHpA (Alias: CN2237) Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium ...

... FAST system, Ye’s lab could edit a tdTomato fluorescent reporter gene in mice using Minicircle DNA vectors...of Cas9 and a photo-dissociable dimeric green fluorescent protein (pdDronpa) (Zhou et al., 2018). pdDronpa... flanking the DNA-binding cleft of Cas9, the fluorescent domains dimerize and prevent DNA binding in the...chromatin architecture (Hoffman et al., 2020). Fluorescent dCas9 proteins can be used to identify specific...gRNA and a dCas9 fusion protein with three red fluorescent tags in U2OS cells. Upon light activation, dCas9...figure below. Next, by using a dual reporter fluorescence assay, Hemphill et al. demonstrated that the...

...Which Fluorescent Protein Should I Use? Plasmids 101: Green Fluorescent Protein (GFP) Fluorescent Protein...Plasmids 101 has covered GFP and its related fluorescent proteins, which are sometimes used as tags for...tag for purification? Or you want to combine a fluorophore with a tag that localizes your protein to the...

...better ability to record subthreshold voltage fluctuations, while having high fidelity during action potentials...Structure of Voltron or Voltron2 conjugated to a JaneliaFluor dye emitting at 525 nm. Image reused from https...Because they are negative-going sensors (with fluorescent signal at baseline voltage), the investigators...closely-packed labeled cells. Plus, Voltron2’s fluorescence is tunable by changing the dye that becomes ...6).   Figure 6: Illustration of split fluorophore assay to test reconstitution via mRNA trans-splicing...

...The SunTag system uses an scFv to amplify the fluorescence intensity of a tagged protein. SunTag has two...bind the SunTag scaffold, the intensity of the fluorescent signal is boosted and enables single molecule...contains an HA-binding scFv that’s fused to a fluorescent protein such as GFP. The HA frankenbody works...frankenbodies, an anti-HA scFv-based probe for fluorescent imaging. Learn more about antibodies  Read about...SunTag system which uses an scFv to amplify the fluorescence intensity of a tagged protein. Resources on...

...switch to turn recipient cells from red to green fluorescence (Figure 2). Donor cells express iCre with a ...GECIs have been derived from just a few parent fluorescent proteins, PinkyCaMP is the first based on mScarlet...team could multiplex PinkyCaMP alongside green fluorescent and optogenetic tools. FRCaMPi uses an inverted...et al. (2025). A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal...expressing the protein of interest fused to GFP forms fluorescent biomolecular condensates. A GFP-nanobody fused...

...transporters. The intensity-based Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by ...primary visual cortex (V1) layer 2/3 (L2/3). B) Fluorescence from axonal boutons of neurons expressing iGluSnFR3... IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Thermo Fisher A-21131) or Goat anti-Rabbit ... (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (Thermo Fisher A-11012), and counterstained ... optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato under the control of one of ...

...26322837. 2. Stadler, Charlotte, et al. "Immunofluorescence and fluorescent-protein tagging show high correlation...displayed good binding efficiency in both immunofluorescence and immunoprecipitation applications, but...Plasmids Tag endogenous proteins with CRISPR Fluorescently Tag Your Protein of Interest ...

...with Rosella, a pH-sensitive fluorescent biosensor A pH-sensitive fluorescent biosensor-based assay system...expression constructs fused with epitopes or fluorophores, generate expression vectors with various promoters...DsRed is relatively pH-insensitive, while SEP fluoresces at cellular pH (7.0) but not at vacuolar pH (...environments that inactivate SEP and leave only DsRed to fluoresce (1). With variants that can be targeted to specific...tagging event. Commonly used sequences, including fluorescent tags, a floxed Cbr-unc-119 selectable marker,...the mAID vectors come with mCherry2 or mClover fluorescent proteins allowing you to screen for fusions. ...

...element (tyrosine sulfation consensus sequence, fluorophore, or APEX2), allowing for quantitative analysis... Since the anti-GFP nanobody is able to bind fluorescent proteins derived from A. victoria the functionalized...fused with GFP, YFP, mCerulean, or other related fluorophores. Find all of the nanobody toolkit plasmids here...technologies to quantify proteins in tissues, cells, and biofluids. Recently, Sam Myers, a Scientist in the group...

...mCherry fluorescent protein followed by a stop codon and the coding sequence for a green fluorescent protein...representation of cell-free system, capable of producing fluorescent proteins and enzymes. Image from Moore et al....validated through monitoring the expression of fluorescent proteins and oxytetracycline enzymes. Next, this...

...recent contribution to the field is a series of fluorescence resonance energy transfer (FRET)-based calcium...with a solution: they devised self-associating fluorescent domains whose association is disrupted in the...Robust red FRET sensors using self-associating fluorescent domains. ACS Chem Biol. 2013 Oct 18;8(10):2133...et al. MagFRET: The First Genetically Encoded Fluorescent Mg(2+) Sensor. PLoS One. 2013 Dec 2;8(12):e82009...

...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable... sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to...: Epigenetics Visualizing Genomic Loci Using Fluorophores CRISPR imaging has numerous advantages, including...customizable DNA or RNA label for fluorescence microscopy. RNA aptamers, like MS2 or PP7, can also be fused...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging...dCas9 and S. aureus dCas9) tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal...imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent CRISPR systems have been used for dynamic tracking...

...Genetically encoded voltage indicators (GEVIs) change fluorescence in real time when neurons or muscle cells transmit...However, technical difficulties such as small fluorescence changes or a requirement for very high excitation...sensors can detect action potentials in worms with fluorescence changes of up to 128% per 100 mV in the pharynx.... elegans membranes, which might beneficially influence the rhodopsin properties. Find these voltage biosensors...