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We narrowed to 1,144 results for: Gene

Showing: 161 - 180 of 1144 results

  1. Lentivirus Plasmids

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    Collection
    ...viral particle) and toxicity of the gene product (i.e., if the gene encoded by the transfer plasmid is ... which can be modified to encode a wide range of gene products. The main restrictions on a lentiviral ...chimeric 5’LTR, Any expression cassette (promoter and gene of interest) can be cloned into the plasmid. Nolan...gateway plasmid, constitutive Hygromycin resistance gene coexpression. See article for other versions of ...pULTRA 3rd bi-cistronic expression of EGFP and the gene of interest, also can express shRNA (cloning H1-...mutagensis. The 2nd and 3rd generation lentiviral systems available at Addgene have been designed for increased...used with 2nd and 3rd generation lentivirus technologies. ID Plasmid Generation Type Description PI 12253...

  2. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    Blog Post
    ...Reporter Genes (ARGs)? Fluorescent and bioluminescent reporter genes are widely used to study gene expression...for visualizing gene expression in vivo.  But what ultrasound lacked was reporter genes that could produce...Lab - developed Acoustic Reporter Genes (ARGs), which are sets of genes that, when expressed, enable cells...vesicles gene clusters from a diverse set of gas vesicle-expressing species. The gas vesicle genes from Serratia...structural gene gvpA marked with EBFP2 and the second cassette contains the P2A-linked accessory genes gvpNJKFGWV...first-generation mammalian ARGs (mARGs) enabled ultrasound visualization of in situ mammalian gene expression...hypothetical protein, improving expression, and named this gene set bARGSer, for bacterial ARG from Serratia sp....

  3. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

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    Blog Post
    ...find AAV tools for optogenetics and chemogenetics on the Addgene website. Optogenetics also requires that...molecule activation (chemogenetics) or light (optogenetics).   Timing Optogenetics offers a high degree...limitations.  The four main factors in which chemogenetics and optogenetics differ are: Timing Targeted manipulation...schematic showing the differences in chemogenetics and optogenetics in a mouse experiment. Viral delivery...faster.  Chemogenetics does not offer the same level of control over timing. Because chemogenetics relies...required. Targeted manipulation Both optogenetic and chemogenetic tools can have their expression targeted...stimulation is gradual. Invasiveness Both optogenetic and chemogenetic tools need to be delivered to cells,...

  4. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post
    ...resistance gene, NPTII, facilitates phosphorylation of kan, which inactivates it. The NPTII gene confers ...death by intercalating into DNA and generating DNA breaks. The Sh ble gene provides zeocin resistance by expressing...the Sh ble gene does not fully suppress Zeocin-induced DNA cleavage in human cells. Mutagenesis, 20(2), ...Plasmids need antibiotic resistance genes so that you can specifically isolate bacteria containing your.... But does it matter which antibiotic resistance gene you select? In this blog, we’ll break down the mechanism...action of the most common antibiotic resistance genes and which applications are best (or worst) for each...plasmid, isolate individual plasmids, or express a gene contained in a plasmid. During the propagation and...

  5. Antibodies 101: Isotypes

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    Blog Post
    ...constant genes μ and ẟ. These are the first two genes in the heavy-chain constant domain gene cluster....constant domain genes, placing the variable domain genes next to a different constant domain gene. This process...Heavy chain constant genes are clustered together downstream of the variable region gene in this order: μ,...constant domain gene. For example if a B cell switches to producing IgE antibodies, the gene segments for...nomenclature. Antibody heavy chain proteins as well as the genes that encode those proteins are designated by the...μ, δ, γ, ε, and α. An antibody variable gene is initially expressed with the IgM and IgD constant domains...domains because they are the first constant domain genes in the cluster. However, when B cells switch to ...

  6. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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    Blog Post
    ...The white gene is most often used, but other selection markers include vermillion (another gene controlling...as species of gene. Find Gal4 and UAS plasmids at Addgene Choosing a vector to generate a transgenic fly...described the Gal4/UAS system used by geneticists to study gene function. In this final installment, I...you’d like to use to generate your new fly line: 1. Where would you like the gene to be incorporated into...spot flies with the appropriate transgene. One such marker is the white gene, which restores eye color to...engineer new transgenic flies to study your favorite gene (YFG).  Sometimes, you want to use the Gal4/UAS...fairly straightforward. Basically, you place your gene of interest in a plasmid and get the vector incorporated...

  7. CRISPR Plasmids - Purify Genomic Loci

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    ... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter...Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  8. CRISPR Plasmids - RNA Targeting

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    Collection
    ...ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid Gene/Insert PI Publication... Publication Plant ID Plasmid Gene/Insert PI Publication Do you have suggestions for other plasmids that...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  9. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

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    Blog Post
    ... Acoustic reporter genes (ARGs) Acoustic Reporter Genes (ARGs) are engineered gene clusters that encode...for expression in E. coli: acoustic gene 1 (arg1) and acoustic gene 2 (arg2). The key difference between...Resources on the Addgene Blog Learn more about in vivo imaging tools The luciferase reporter gene The green ...green fluorescent protein reporter gene Resources on Addgene.org Find plasmids for in vivo imaging with ... reporters has its drawbacks. Acoustic reporter genes (ARGs) overcome these limitations by using gas vesicle...and high spatial resolution. By piecing together genes from gas vesicle operons from two different bacteria...their ultrasound signal. Using acoustic reporter genes for in vivo imaging Following in vitro characterization...

  10. PiggyBac-ing Through the Genome Editing Field

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    Blog Post
    ...numerous applications, including transgenesis, gene-trap screens, and gene editing. How does the piggyBac...levels of your gene of interest, as needed. Controlling the expression levels of your gene may be particularly...Transposagen.   How does piggyBac™ enhance gene editing? Gene editing can now be accomplished in a more... How does the piggyBac™ gene editing system compare with other methods of gene editing? There are primarily...Decoding the Epigenetic Heterogeneity of Human Pluripotent Stem Cells with Seamless Gene Editing.,” Methods...remain, such as making gene edits completely seamless (i.e., so no other genetic alterations have occurred...cell lines and genetically-modified cell lines. Furthermore, the use of piggyBac™ for gene editing allows...

  11. DAP arrays for multiplex gene editing

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    Blog Post
    ...any CRISPR-based gene editors, for correcting mutations or installing functional genes to study and potentially... number of genes with a single viral vector.   Fig. 1: Schematic of multiplex gene editing with...enable multiplex gene editing in a variety of cells and organisms. However, lengthy genetic payloads and ...demonstrating simultaneous editing of genes against multiple genetic diseases, such as muscular dystrophies...limited the scalability and efficiency of multiplex gene editing. Moreover, existing multiplex strategies...single array, has been a top choice for multiplex gene knockout or regulations, it lacks proper functions...derived tools are leading the edge of the precision gene editing. CRISPR-Cas9, the key component to enable...

  12. An Introduction to Adenovirus

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    ...Adenovirus Vectors for Gene Therapy, Vaccination and Cancer Gene Therapy. Curr Gene Ther, 13(6), 421–433...compartment, gene expression, and protein translation. Its genome consists of “early” (E) and “late” genes, and...with the E1 and E3 (Early 1 and Early 3) genes deleted. These genes’ functions are rescued by helper plasmids... be expressed, since the early genes are absent, and the late genes (and their structural proteins, which... does not require de novo viral gene expression: Implications for gene therapy. Proc Natl Acad Sci U S...house a recombinant sequence (transgene) instead.    Although the late genes are usually present in the ...and E3 gene complementation. In target cells that are infected by the vector, only the transgene will be...

  13. Plasmids 101: The Wide World of Natural Plasmids

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    Blog Post
    ...hosts through horizontal (or “lateral”) gene transfer. Horizontal gene transfer can occur naturally through...carrying genes for bacterial toxins like colicin typically also carry the necessary resistance genes to help...Additional Resources on the Addgene Blog Plasmids 101: Antibiotic Resistance Genes Antibiotic Resistance: ...cell to another). Bacteria need special fertility genes to be capable of conjugation, and these are encoded...there is evidence that lightning can also promote gene transfer in soil bacteria! (Demanèche et al., 2001...organisms to thrive in competitive environments. The genes encoding these functions form the basis of many ... Resistance (R) plasmids: microbial defense Genes allowing bacteria to survive toxins are the clearest...

  14. A new platform for fast functional characterization of potentially cancerous mutations

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    Blog Post
    ... several genes spanning 33 cancer types. The types of mutations they identified included gene amplifications... plasmids each carrying a single gene with a defined mutation or gene fusion. Plasmids carrying the corresponding...how a given gene is involved in a complex pathway. Some of the most heavily-studied genes in cancer research...help cancer researchers studying an assortment of genes. Using patient data to identify mutations of unknown...corresponding wild-type genes were constructed as well. They called this library the CTD2 FASMIC Plasmid...thoroughly investigate any of the dozens of wild-type genes and their associated mutants in other model systems... Yiu Huen, et al. "Functional annotation of rare gene aberration drivers of pancreatic cancer." Nature...

  15. Five Popular Model Organisms

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    Blog Post
    ...spatiotemporal expression of a gene of interest. This type of control over gene expression is possible in ...diseases that are caused by a specific gene. For example, several genes mutated in human cancers have a homolog... They share about 70% of their genes with humans and 85% of human genes associated with a disease have... share many genes with humans, are easily maintained in the lab, and have short generation times that ...Thus any genetic modification, such as expression of fluorescent protein or disruption of a gene, can be...Refinement of tools for targeted gene expression in Drosophila." Genetics 186.2 (2010): 735-755. PubMed ... of Drosophila research. Morgan discovered that genes were found within chromosomes using the fruit fly...

  16. Plasmids 101: Modular Cloning

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    Blog Post
    ...platform. Gene Stacking As the name implies, gene stacking is the use of a combination of multiple genes to ...regulate the transcription of specific genes. What if one of those genes coded for a protein that regulated...combinations of different candidate genes at once to identify the best cocktail of genes when the relationship between...transcription of another gene? Now you have a genetic circuit! Genetic circuits are multigene systems whose protein...characterized activities with predictable behaviors. While a gene is the most obvious example of an individual part...transcribed together. They can express one or more gene(s), and typically begin with a promoter and end ...could be comprised of just three parts: promoter, gene, and terminator.     Fig 1. MoClo enables...

  17. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    Blog Post
    ...strategy is to knockdown a gene with RNAi. Here, a short sequence targeting the gene is cloned into a viral...whether it is an overexpression gene, downregulation via RNAi or gene editing/deletion using CRISPR. In...conjunction with a genetic manipulation so that behavior can be linked to a specific gene or brain region...viral vector should be used? Modifying gene expression Generally speaking, one may want to achieve either... application.  Genetic manipulation can also reduce expression levels of certain genes. A popular method...can now precisely control where and when certain genes are expressed to investigate their role in a specific...specific brain region. One option is to overexpress a gene that alters neuronal function directly such as receptor...

  18. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    ...Positive-selection vectors using the F plasmid ccdB killer gene”. Gene. 1994 Oct 11;148(1):71-4. PubMed PMID: 7926841... experiments, and here we will focus on a unique gene that makes the popular GatewayTM method possible...accomplish this? Let’s start with a brief history of the gene and how molecular biologists have harnessed it to...cloning technology. CcdB: A potent toxin... The ccdB gene, located on the F sex factor plasmid of E. coli,...and ultimately causing cell death. ccdA, another gene found in the ccd operon, codes for the antitoxin...vectors, called pKIL18 and pKIL19, contained the ccdB gene in frame with an MCS. E. coli that were transformed...transformed with the empty vectors expressed the ccdB gene and were therefore unable to propagate because CcdA...

  19. Plasmids 101: FLEx Vectors

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    Blog Post
    ... the gene you would like to rescue with in the antisense orientation (let’s call the gene “GeneR”). The...rescue of a gene knockout: To test if the phenotype you are observing is really due to a single gene deleted...endogenous gene. Spatio-temporal expression of optogenetic reporters: Since most optogenetic reporters...transduced reporter gene. Generating a FLEx switch to control expression of an optogenetic reporter would ... were designed as a genetic tool for researchers to conditionally manipulate gene expression in vivo using...conditionally turn off one gene and activate another. In the field of mouse genetics, FLEx is currently being... vivo conditional gene rescue experiments, as well as for improvement of optogenetic adeno-associated ...

  20. Plasmids 101: Gateway Cloning

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    ...recombination; ccdB gene for negative selection Used to clone attB-flanked genes of interest to generate entry clones...for recombination; ccdB gene for negative selection; elements to express the gene of interest in the appropriate...for gene expression.     The LR Reaction takes place between the attL sites of the generated entry...attB sites is generated. As in the BP reaction, a DNA fragment containing the ccdB gene is excised from... cloning to generate our desired constructs: lentiviral expression of the human KRAS gene in mammalian...   Pro Tip: Addgene also has ready-made entry clones available for many popular genes, including Hs....our website to search for your favorite gene! STEP 2: Generate an Expression Clone When making the expression...
Showing: 161 - 180 of 1144 results