We narrowed to 1,082 results for: IND

...Addgene's zebrafish resources: Find plasmids for use in zebrafish....

...about this field. Use the table and filters below to find ready-to-use AAV preparations of optogenetics plasmids...

... Protocols Ligation Independent Cloning Ligation Independent Cloning You...for Ligation Independent Cloning Protocols...Molecular Biology Protocols Overview Ligation Independent Cloning (LIC) obviates the need for the time-...

...Validation Using the Indirect ELISA Method Antibody Validation Using the Indirect ELISA Method You may...This protocol describes how to run an indirect ELISA against a purified antigen to demonstrate the expected...Introduction This protocol describes how to run an indirect Enzyme-Linked ImmunoSorbent Assay (ELISA) against...display a green product. A precipitated product indicates the presence of too much HRP and the need to optimize...

...publically available through Addgene. Stovicek, et al. J Ind Microbiol Biotechnol. 2015. PubMed PMID: 26376869...Toolkit Many industries, such as the food and beverage industry, the bioethanol industry, and the pharmaceutical... make it easier for you to find and use the plasmids you need. You can find all the hot plasmids from ...Nile Virus (WNV) induced cell death. The Wu lab library consists of 77,406 individual gRNAs targeting ...optimizations are required for each individual target species. Indeed, in order to use MAGE, the λ Red recombinase...for genetic optimization of industrial yeast strains. However, industrial yeast strains are more difficult...Targets of RNA-binding proteins identified by editing Identifying targets of RNA-binding proteins (RBPs...

...FKBP rapamycin binding domain of mTor (Cas9(N)-FRB-NES) resulting in a rapamycin-inducible Cas9 for genome...Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885 & 62886) Rapamycin-inducible dCas9-VP64...domain unfolds allowing it to bind to SspB. This original Light Inducible Dimer (oLID) could be used to... not show large changes in binding affinity with light stimulation. Indeed oLID showed only a two-fold...throughput binding assays, to engineer photoswitchable dimers which show over a 50 fold change in binding affinity... coat protein binding sites and 24 copies of the MS2 bacteriophage coat protein binding sites to tether...constitutive or inducible, gene knockdown with shRNA or shRNA-miR30, (2) tamoxifen-inducible CreERT2 gene...

...ON. When a repressor binds the activator protein, the activator protein cannot bind the promoter sequence... cannot bind the promoter sequence and transcription is ON. Once a co-repressor protein binds the repressor...derivatives is added, it binds tTA, resulting in a confirmation change that prevents binding to the promoter and...cumate is added, it binds cTA, resulting in a confirmation change that prevents binding to the promoter and.... As ethanol accumulates, it binds to the repressor, enabling it to bind the promoter and repress pADH1...system). The GAL80 repressor can bind to GAL4, partially inhibiting the binding of GAL4 to UAS. One can therefore...manner, with LexA binding to the lexA operator (lexAop). Fusing the LexA DNA-binding domain (DBD) to GAL4...

...interactions. Next comes the binding step where the target antigen binds to antigen-specific antibodies...conjugated antibody binds to antigen coated on the multiwell plate. In an indirect ELISA a primary antibody...antibody first binds to the antigen and then a reporter enzyme conjugated secondary antibody binds to the primary...antibody that binds to the antigen in a sample and then a second detection antibody binds. The detection...less antibody available to bind to the competing antigen. In direct, indirect and sandwich ELISA the reporter...research before conjugating at the bench.   Indirect ELISAs The indirect ELISA process is similar to that of ... easy to find,  it is often easier for labs to source appropriate antibodies for an indirect ELISA than...

...dark-induced dark-induced dark-induced Loop 6 blue-light induced dark-induced blue-light induced Table...controlled binding, but with opposite effects: insertion into loop 1 caused dark-induced binding while loop...OptoNBs with dark-induced binding, but insertion into loop 6 generated both blue light inducible and dark-inducible...OptoNB can bind, it co-localizes with its target at the cell membrane and when the OptoNB can’t bind, it localizes...loop 6 insertion caused blue-light induced binding. The lab used this general design scheme to generate...the effect of AsLOV2 insertion site on light-induced binding.  Light controlled protein purification with... path to light-controlled protein binding, they probably don’t bind your favorite protein yet. More OptoNBs...

...While a primary antibody binds to the protein of interest, a secondary antibody binds to the primary antibody...primary antibody, due to increased binding affinity, many indirect antibody protocols are designed to ...anti-mouse secondary will bind to all the primary antibodies indiscriminately, giving them the same output...antibody with a signaling molecule, the antibody binds to a protein, and then voila! Wherever your protein...protein of interest, it may be time to look at the indirect approach - which means it’s time to talk about...antibody. In the indirect approach, the signaling molecule is conjugated to the secondary antibody instead...antibody. Since multiple secondary antibodies can bind to a single primary antibody, this approach can ...

...attachment. The five other IPI rAbs bind RGD-binding integrins at non-ligand-binding sites and do not interfere...morphology changes induced by integrins binding the RGD motif compared to those binding the Leucine-Valine-Aspartate-Proline...integrin antibodies exist, almost all bind outside the ligand-binding pocket, their sheer size impeding ligand... investigators inhibited other RGD-binding integrins, individually and in combination, showing that among...study. Cells often express multiple integrins that bind the same ligand, making it difficult to dissect ...The collection includes 26 unique antibodies that bind discrete subunits or particular subunit pairs. Some...antibodies in the collection are able to block ligand binding — and therefore interrupt integrin function — providing...

...SF-iGluSnFR. Image from Aggarwal 2022.   Find iGluSnFR3 plasmids here! Find viral prep here: pAAV.hSyn.FLEX.iGluSnFR3...al. 2022, under CC-BY-NC license.   Find JEDI-2P plasmids here! Find viral preps here: pAAV-EF1a-DIO-JEDI...this in mind, Ed Boyden and colleagues set out to engineer a potassium ion biosensor with a binding affinity...of potassium binding proteins, they developed a family of fluorescent potassium indicators with a range...new tools to measure their favorite targets and finding new targets to measure! Let’s take a look at some...adapted from Ino et al. 2022, under CC-BY license.   Find MTRIA sensors here!   Ino D, et al. A fluorescent...doi: 10.1038/s41592-022-01597-x. New glutamate indicator iGluSnFR3 Improving on the glutamate sensor iGluSnFR...

...transferred to an industry position. If you cannot get an Industry H-1B, remember that industry career paths...Subject Industry H1B Academic H1B National Interest Waiver EB-1A EB-1B Cap subject industry H-1B There...process or quota like industry, these organizations can be a good choice for individuals needing sponsorship...importance of the individual’s work in their field and the impact of the individual’s past work on the ...passionate about working with scientific individuals to find exciting growth opportunities in life sciences...growing shift away from employers sponsoring individuals for green cards because of the cost, long timeline...available to you including careers in academia, industry, non-profit, and government sectors.  Within these...

...canonical base editing window, base editing has higher efficiency and fewer indels than prime editing. But...increase activity, enhance binding between the template and primer binding site, increase processivity...nicking increases indel formation slightly. Designing the sgRNA with a spacer that only binds the edited strand... the indicated tool. ~ reflects an innovation that may optionally be combined with the indicated tool.... advances in this evolving field! Find prime editing plasmids! Find plasmids from David Liu's lab! This...developed base editors with a <8 base-pair editing window cannot edit within what Fyodor Urnov refers to ...edit all the target bases within the base editing window, while prime editors make a specific edit defined...

...too common for antibodies to bind to off-target molecules or even to not bind to their target molecule at... to make sure that your antibody binds to what you expect it to bind to. Show specificity for the target...because an antibody can bind to its target, does not mean that is all it will bind to - particularly when...of antibody-independent approaches to your antibody-dependent approach. Antibody-independent methods may...different tissues or cell types. If your antibody indeed binds to your target then you would expect to see ...influence antibody binding by altering the relative concentration of potential antibody binders and so this ...of the researcher toolkit due to their ability to bind to molecular targets with high affinity and specificity...

... cut site.     Indel Distribution In the Indel Distribution tab, you’ll find an Indel plot (left panel... 21+ bp indel. This score is a useful measure to determine how many of the contributing indels are likely...deletions (indels) and then characterizes the sequence and abundance of each particular indel.   For a ...processing, the window shows a yellow checked circle. Typically, a yellow check mark indicates that a particular...will open up a new window with three tabs.  Each of the three tabs - Contributions, Indel Distribution and...distribution of indel sizes in the entire edited population of genomes. Hovering over each bar of the indel plot ... Check out our CRISPR guide Find CRISPR plasmids for your research Find other CRISPR software resources...

...make it easier for you to find and use the plasmids you need. Below you'll find our hot plasmid articles...chemically inducible approaches to provide temporal control, this new system, Fkbp/Frb Inducible Recruitment... GAL4 or ZIF268 DNA binding domains fused to either the hER or hPR ligand binding domains and the MSN2...expression is dependent only upon its cognate inducer. Since induction via estradiol and progesterone do not ...transcription factors in series and independently changes inducer levels. That is, if transcription factor...created stable cell lines overexpressing TRIM21, finding that TRIM21 overexpression did not affect cell ...changes in expression of TRIM21-associated genes, indicating that TRIM21 overexpression does not aberrantly...

...arrows indicate a conversion directly catalyzed by the base editor. Dotted red arrows indicate the change...well as a 2.3 fold decrease in indel formation. To further decrease indel formation 1.5-2 fold, the team...editing window of target positions 4-7. ABEs 6.3, 7.8, and 7.9 display slightly wider editing windows of position...but higher indel formation than BE2 Nishida et al., 2016 Target-AID   Edits 3-5 base window surrounding...following base editing and DNA repair. Black arrows indicate natural cellular processes. BER = base excision...locus A target base for editing within the editing window specified by the Cas protein These elements were...of targets in human cell lines, with an average indel frequency of only 1.1%. These numbers are a vast...

...protein DnaA. First, DnaA must bind ATP. Then, the ATP-bound DnaA binds multiple specific sites within...controlled plasmids. Binding of IHF (green) redistributes DnaA (red) from binding primarily high-affinity...protein binding it (Grimwade et al., 2018). Rather than serving as an energy source, ATP binding is one...interacts with DnaA, stabilizing its binding and contributing to DNA unwinding. This promotes initiation at the... plasmids that can initiate DNA replication independently are said to be under relaxed control, as they...come in both high- and low-affinity versions, but binding of the low-affinity versions is required to start...DnaA alone, as long as there are enough copies to bind every available DnaA box. In bacteria, however, ...

... make it easier for you to find and use the plasmids you need. You can find all the hot plasmids from ...SpCas9 (NGG)  Editing window ~2 nt  85175  pBK-EE-BE3  SpCas9 (NGG)  Editing window ~2 nt  85176  pBK-YE2...essential in B. subtilis 168. Each individual strain includes a xylose-inducible dCas9 paired with a single, ...found that TZAP binds directly to TTAGGG repeats via its zinc finger domains. This binding was also independent...independent of another TTAGGG binding complex, shelterin. Swapping the DNA binding domain of another telomeric...N-terminal variants on iPSC generation Deriving iPSCs (induced pluripotent stem cells) through the expression ...valuable tool for the research community. You can find the AAV vectors for infecting GABA-ergic interneurons...