We narrowed to 214 results for: Kars

1. Plasmids 101: Common Lab E. coli Strains

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   Blog Post

   ...let’s take a moment to recognize your little prokaryotic minions that carried out the labor-intensive ...

2. Antibodies 101: Multiplex Immunofluorescence

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   Blog Post

   ...., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E. M., & Weissgerber, T. L. (2021...

3. CRISPR 101: Epigenetics and Editing the Epigenome

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   Blog Post

   ...dCas9 to a small DNA methyltransferase from the prokaryote Mollicutes spiroplasma (M. Sss1) (termed MQ1....

4. Generating Mouse Models Using CRISPR/Cas9

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   Blog Post

   ... it has been repurposed for genome editing in eukaryotes, with the most widely used CRISPR genome editing...

5. Building Global Connections with the International Mentorship Program USA-EUROPE

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   Blog Post

   ...Hopkins University, the Salk Institute, and the Karolinska Institutet, among others). Mentors and mentees...

6. Brain Initiative Collection

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   ... PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Cre-dependent recombination...

7. When Fidelity Matters: A frank discussion about ligase fidelity

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   Blog Post

   ...  3. Ellenberger, Tom, and Alan E. Tomkinson. "Eukaryotic DNA ligases: structural and functional insights...

8. RNA Interference in Plant Biology: New Tools for an Old Favorite

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   Blog Post

   ...number. RNA interference (RNAi) is a conserved eukaryotic process where approximately 20-30 nucleotides...

9. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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   Blog Post

   ...mycoplasma enzymes that are not synthesized by eukaryotic cells. First, the kit components rupture the ...

10. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    Blog Post

    ...:18658221. PubMed Central PMCID:PMC2527278. 9.Karbowski, Mariusz, et al. "Quantitation of mitochondrial...

11. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post

    ...Protein expression in yeast MoClo is also for eukaryotes! The technology has been used to optimize protein...

12. Cancer and the Immune System: Deciphering the Relationship

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    Blog Post

    ...): 517-528. PubMed PMID: 25319501. 7. Palucka, Karolina, and Jacques Banchereau. "Cancer immunotherapy...

13. A Primer on Optogenetics: Introduction and Opsin Delivery

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    Blog Post

    ...elsewhere (here and here for instance) [2], [3]. Karl Deisseroth of Stanford University , one of the key...

14. Selecting Your Plasmid Purification Kit

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    Blog Post

    ...bacteria, a few manufacturers (such as Zymo and TakaraBio) offer yeast-plasmid specific kits.  Medium/High-throughput...

15. CRISPR 101: Multiplex Expression of gRNAs

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    Blog Post

    ...polycistronic glycine tRNA-gRNA (PTG) constructs. Eukaryotic RNases P and Z recognize the tRNA sequences, ...

16. Delivery Methods for Generating iPSCs

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    Blog Post

    ...Minicircles are like mini-plasmids. They contain only a eukaryotic promoter and the cDNA(s) to be expressed and ...

17. CRISPR 101: Cytosine and Adenine Base Editors

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    Blog Post

    ...2016). Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science...

18. Fluorescent Protein Guide: Biosensors

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    ...Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression...Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. Karl Deisseroth , INTRSECT 2.0 Project Calcium Mitochondrial...

19. Using AAV for Neuronal Tracing

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    Blog Post

    ...Hantman, A.W., Looger, L.L., Schaffer, D.V., and Karpova, A.Y. (2016). A designer AAV variant permits efficient...

20. CRISPR Plasmids - Parasites

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    ...Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic parasites continue to cause substantial death...