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Addgene
Showing: 161 - 180 of 207 results

  2. Viral Production

    Type
    Collection
    ...detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). ...Mycoplasma The 293T cell line was obtained from Takara, and is routinely tested for mycoplasma contamination...

  3. Tetracycline Inducible Expression

    Type
    Collection
    ...of inducible promoters capable of controlling eukaryotic gene expression. Several endogenous promoters...Scientists began to pursue a non-endogenous system for eukaryotes. At the time, bacterial systems from E. coli ...

  11. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...better for PCR and surveyor. I used DNA polymerase Takara Ex Taq ™ DNA Polymerase for my genomic PCR, but...successfully used Herc II or Kapa Hifi in PCR. Maybe this Takara enzyme is not very robust in this case for EMX1...

  14. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...please try viewing on YouTube . Abstract The prokaryotic clustered regularly interspaced short palindromic...9 system may be re-purposed for site-specific eukaryotic genome engineering. CRISPR/Cas9 is an inexpensive...

  15. Luciferase Plasmid Collection

    Type
    Collection
    ...luciferase. Renilla luciferase is co-expressed. Oskar Laur 87070 pcDNA3.1-Nanoluc-ccdB NanoLuc® Creation...luciferase from broad host range plasmid Attila Karsi 105621 Lenti-luciferase-P2A-Neo Firefly EF1α Lentiviral...

  18. Brain Initiative Collection

    Type
    Collection
    ... PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Cre-dependent recombination...

  19. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...Hantman, A.W., Looger, L.L., Schaffer, D.V., and Karpova, A.Y. (2016). A designer AAV variant permits efficient...
Showing: 161 - 180 of 207 results