Evolution of Lab Techniques
Type
Blog Post
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4. Haruyama, Naoto, Andrew Cho, and Ashok B. Kulkarni. "Overview: engineering transgenic constructs ...
We narrowed to 206 results for: Kars;
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Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...Chen Y, Fischer T, Tedaldi E, Napoli A, Zhang Y, Karn J, Hu W, Khalili K. Elimination of HIV-1 Genomes... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. In vivo genome... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...2010) and the MAGIC Marker strategy described in Karine Loulier, et al. (2014). Currently, Brainbow techniques... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog Post...let’s take a moment to recognize your little prokaryotic minions that carried out the labor-intensive ... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...Hantman, A. W., Looger, L. L., Schaffer, D. V., & Karpova, A. Y. (2016). A Designer AAV Variant Permits Efficient... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...dCas9 to a small DNA methyltransferase from the prokaryote Mollicutes spiroplasma (M. Sss1) (termed MQ1.... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post...., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E. M., & Weissgerber, T. L. (2021... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post... it has been repurposed for genome editing in eukaryotes, with the most widely used CRISPR genome editing... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post...Hopkins University, the Salk Institute, and the Karolinska Institutet, among others). Mentors and mentees... -
Brain Initiative Collection
TypeCollection... PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Cre-dependent recombination... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog Post...number. RNA interference (RNAi) is a conserved eukaryotic process where approximately 20-30 nucleotides... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post...mycoplasma enzymes that are not synthesized by eukaryotic cells. First, the kit components rupture the ... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog Post... 3. Ellenberger, Tom, and Alan E. Tomkinson. "Eukaryotic DNA ligases: structural and functional insights... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...:18658221. PubMed Central PMCID:PMC2527278. 9.Karbowski, Mariusz, et al. "Quantitation of mitochondrial... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post...Protein expression in yeast MoClo is also for eukaryotes! The technology has been used to optimize protein... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog Post...): 517-528. PubMed PMID: 25319501. 7. Palucka, Karolina, and Jacques Banchereau. "Cancer immunotherapy... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...elsewhere (here and here for instance) [2], [3]. Karl Deisseroth of Stanford University , one of the key... -
Selecting Your Plasmid Purification Kit
TypeBlog Post...bacteria, a few manufacturers (such as Zymo and TakaraBio) offer yeast-plasmid specific kits. Medium/High-throughput... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...polycistronic glycine tRNA-gRNA (PTG) constructs. Eukaryotic RNases P and Z recognize the tRNA sequences, ...