We narrowed to 220 results for: LRE

1. Plasmids 101: Colony PCR

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   ...expected size, you know the genome of your bacteria already contains your target sequence. Additional Resources...

2. Plasmids 101: Protein tags

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   ... cannot be used in eukaryotes because there is already too much SUMO protease around, but it is convenient...

3. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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   ...beauty of embryo development in real time. I was already a biology geek, but this formative experience nailed...

4. Lab to Office Culture Shock

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   ...expect during the lab → office transition? I’ve already provided a few counterpoints to some of the causes...

5. Supporting Reproducibility with a Connected ELN

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   ...repository. The ability to deposit data that has already been structured in folders or notebooks in the ...

6. Transferable Skills Guide: External Collaborations

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   ...collaboration management. For example, you may already be part of a scientific collaboration - show initiative...

7. Rett Syndrome: A History of Research and Therapeutic Outlooks

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   ...Cell Reports 32:107878 . https://doi.org/10.1016/j.celrep.2020.107878 Sinnett SE, Hector RD, Gadalla KKE...

8. To Codon Optimize or Not: That is the Question

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   ... no need to codon optimize as this gene should already be biased to that organism's codon usage. Others...

9. New CRISPR Tools: Cas7-11 and PASTE

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   ...dependent on the cell’s intrinsic machinery being already activated to work. (It was also found to be independent...

10. Antibodies 101: The Four ELISAs and When to Use Them

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    ...Advantages Disadvantages Direct Fast and simple protocolReduced chance of cross-reactivity Higher level of...

11. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    ...was doing the kind of work my lab wanted to do already seemed to know each other and what to do.  Saved...

12. Transferable Skills Guide: Career Planning Resources

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    ...to do this first for companies at which you’ve already made a personal connection. That way, once you’...

13. Antibiotic Resistance: An Old Solution but a New Problem

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    ...Limiting the use of antibiotics in animal farming is already being recognized as an important step, as you can...

14. Changing Labor Laws Bring Increased Postdoc Wages

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    ...Sciences) and the Whitehead Institute (at MIT) all already have salaries at or above $50,000. Boston Children...

15. CRISPR Activation: A Practical Guide

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    ...achieve using a CRISPRa. Your gene of interest might already be hitting an upper bound of activation that current...

16. Data Freedom: The Expansion of Data Sharing in Research Publications

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    ...policy on data access is a formal statement that is already practiced or required by many other journals and...

17. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    ... If the distance is only 10 bp, the efficiency already drops by half and after only about 30 bp, it is...

18. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    ...Cell Reports 20:750–756 . https://doi.org/10.1016/j.celrep.2017.06.064  Carvalho LS, Turunen HT, Wassmer ...

19. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    ...many scFv-based therapies in clinical trials or already in the clinic (Bates and Power., 2019).Many of ...

20. Antibodies 101: Introduction to Gating in Flow Cytometry

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    ... reasonable range. If colleagues in your group already have experience with using flow cytometry, or if...