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Protocol - How to Inoculate a Bacterial Culture

Type

Protocol

...presence of the antibiotic). Luria broth (LB) is a nutrient-rich media commonly used to culture bacteria in...
Centrifugation

Type

Protocol

...Relative Centrifugal Force (RCF) or Revolutions Per Minute (RPM), these are not the same units and are not...
Lab Safety for Biosafety Levels One and Two

Type

Protocol

... in a final concentration of 10% bleach for 30 minutes before pouring down the drain. Solid BSL-2 waste...
Lentivirus Production

Type

Protocol

...Centrifuge the viral supernatant at 2100 rcf for 5 minutes to pellet any packaging cells that were collected...
Antibody Validation Using the Indirect ELISA Method

Type

Protocol

...TMB substrate and the reaction stopped after 15 minutes. Absorbance was read at 450 nm and plotted against...
Isolating a Monoclonal Cell Population by Limiting Dilution

Type

Protocol

...PES filter or centrifugation at >500 x g for 5 minutes. Do not use the medium if the cells are overly ...

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