We narrowed to 207 results for: PHI-1

...plasmids CRISPRi in Caulobacter crescentus Figure 1: Serial dilutions of CRISPRi in Caulobacter cells ... the type II CRISPR3 module of Streptococcus thermophilus (the popular Streptococcus pyogenes system does...

...plasmids. The Zhang lab, on the other hand, uses a 1 plasmid, single infection system. Genome wide mouse...not possible before,” said the Broad Institute's Ophir Shalem. “Whole regions of the genome which were ...

...social media? No problem - see our recap below! Day 1: gRNA design tools: How to Design your gRNA for CRISPR... category. Each plasmid page now has a helpful graphic to introduce you to the plasmid class covered on...

... inhibitory synapses in the brain.  Figure 1:Schematic diagram of different FingR constructs and...over to our CRISPR Plasmids and Resources page.  CasPhi is 70 kDa Cas protein identified exclusively in... RNA targeting Cas system that can be used in Drosophila melanogaster. Get the plasmids at Addgene The...

...started:  Subscribe to Career Blog Posts from Addgene 1) Twitter – Get your voice out there Here’s one thing...involved in education advocacy non-profits like Phipps Conservatory or the Brilliant Club and share your...Radio, WIRED, Scientific American or National Geographic. Similarly, media fellowships from the British...

...in Streptomyces (Cobb et al., 2015). pCRISPomyces-1 includes Cas9, a tracrRNA, and a CRISPR array, while...INTEGRATE consists of four major components including (1) a CRISPR RNA, (2) four proteins forming the QCascade...identified the Cas9 orthologue from Streptococcus thermophilus, Cas9sth1 as a robust and highly active enzyme... al., 2014). These could be genes, specific polymorphisms, including antibiotic resistance genes. This...

...to western blotting  In a western, proteins are: (1) separated by size, (2) transferred to a membrane,...  (3) detected using antibodies.   Figure 1: Overview of the Western blot process. The sample,...sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein...

...in online patient/caregiver communities  Table 1: Examples of defining your audience for science communication...ethnic background, patient or caregiver status, geographic location, sense of humor, media consumed… really...

...could be kept frozen after collection. Figure 1: Paul included a voltmeter on top of the battery box...sampling trip to Yellowstone National Park (YNP). Extremophiles - phylogenetically diverse microbes living in...

...relations, advocacy, and writing legislation (see Figure 1 for career map). The beautiful part of science policy...about science policy as a profession. Figure 1: Science policy can lead you to many paths! For a ...approval for a new product or a well-endowed philanthropy who can shift the funding landscape to focus...

... such a popular model organism? Zebrafish (Figure 1) were initially recognized as a useful vertebrate ...approach that exploits the bacteriophage integrase phiC31. The popular UAS/Gal4 and Cre/Lox gene regulatory...2018): 967-968. PubMed PMID: 29853675. Keller, Philipp J., et al. "Reconstruction of zebrafish early embryonic...

... difficult to modify in the past.   Figure 1: Identification and enrichment use of XMAS-TREE. a...DNA of interest, and cell extract.    Graphical Abstract: Schematic representation of cell-free...

...variants. This resulted in the construction of redCALWY-1, a red-shifted FRET sensor for zinc using variants... his calcium indicators can be used in mouse, Drosophila, C. elegans, and zebrafish – essentially all ...

... cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide analysis...Halstead et al. used TRICK to examine translation of Drosophila patterning gene oskar. Early in development, ...

...defunct (but fun) plug-in called Maps.  Figure 1: See a starfish? This is actually a map of Joanne ...Networking connections can last years This is a graphic snapshot of my LinkedIn network from a few years...

...heterodimerizes with CIBN upon blue light exposure (see Fig 1*). When the group targeted the enhancer region of ...further develop dCas9 for synthetic looping, the Phillips-Cremins lab developed the light-activated-dynamic-looping...

...vector-exosomes), exosome AAVs, or exo-AAVs (Figure 1). The researchers discovered that these particles ...coagulation factor IX in liver in a mouse model of Haemophilia B (Meliani et al., 2017). Exo-AAV9 was successfully...

...individual protein has all the components necessary to: 1. Bind to a guide RNA The guide RNA, which will be ...meningitidis (NM) 3' NNNNGATT  Streptococcus thermophilus (ST) 3' NNAGAAW  Treponema denticola (TD...

...and also by a lactose analogue, IPTG (isopropyl β-D-1-thiogalactopyranoside). (To be completely accurate...reproduce to form colonies (used to complement auxotrophic cell lines), or genes which rescue a lethal phenotype...

...region: IgM, IgD, IgG, IgE, and IgA.    Figure 1: Diagram of an antibody labeled with Fc, Fab, heavy...found bound to IgE receptors on the surface of basophils and mast cells, two types of white blood cells...