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We narrowed to 208 results for: PHI-1

Showing: 161 - 180 of 208 results

  1. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...know that most cephalopods die after they lay their 1 clutch of eggs? Propagation is thus quite difficult...organism blog posts: Five Popular Model Organisms, Part 1 Five Popular Model Organisms, Part 2 ...communities using them grow. So, think beyond Drosophila melanogaster or Saccharomyces cerevisiae (both...

  2. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...Figure 1). Clustered events are called a population.                             Figure 1: While..., Li, J., Greiner, D., & Anderson, D. (2015). Neutrophil Responses to Sterile Implant Materials. PloS ...

  3. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...replication and also act as signals for packaging. Figure 1: Wild-type AAV genome. Created with BioRender.com....extensively studied, and has a broad tissue tropism. Table 1 gives a summary of the tropism of AAV serotypes, indicating...Skeletal muscle AAV1, AAV6, AAV7, AAV8, AAV9 Table 1: Summary of tissue tropism displayed by different ...transgene, but also at a very low efficiency (less than 1% of wild-type). Another split AAV method which has...vectors pseudotyped with viral capsids from serotypes 1, 2, and 5 display differential efficiency and cell...inherited retinal disease, spinal muscular atrophy, hemophilia A and B, and Duchenne muscular dystrophy. Many...

  5. March for Science

    Type
    Blog Post
    ...education, government, and civilization in general (1). Science and politics? Science is an apolitical process...consequences of a march. Three of these risks include: 1) Lost trust Perhaps the greatest risk is that the ...Trump’s immigration ban Studies in History and Philosophy of Science Part: Democratic values and their ...

  6. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...mammalian expression from human elongation factor 1 alpha Constitutive  Tends to give consistent expression... from Drosophila Actin 5c gene Constitutive  Commonly used in expression systems for Drosophila. Polyhedrin...containing Gal4 binding sites, commonly used in Drosophila Specific Requires the presence of Gal4 gene to...

  7. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...downstream experiments. You only successfully generate 1 null clone and proceed with it. In this case, the ...there are 10 putative off-target locations you can 1) determine if editing at those sites would confound...for mismatches within the gRNA sequence. Graphic courtesy of Maya Kostman. The most commonly...

  8. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...plasmids CRISPRi in Caulobacter crescentus Figure 1: Serial dilutions of CRISPRi in Caulobacter cells ... the type II CRISPR3 module of Streptococcus thermophilus (the popular Streptococcus pyogenes system does...

  10. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...social media? No problem - see our recap below! Day 1: gRNA design tools: How to Design your gRNA for CRISPR... category. Each plasmid page now has a helpful graphic to introduce you to the plasmid class covered on...

  11. Hot Plasmids - October 2020

    Type
    Blog Post
    ... inhibitory synapses in the brain.  Figure 1:Schematic diagram of different FingR constructs and...over to our CRISPR Plasmids and Resources page.  CasPhi is 70 kDa Cas protein identified exclusively in... RNA targeting Cas system that can be used in Drosophila melanogaster. Get the plasmids at Addgene The...

  12. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...started:  Subscribe to Career Blog Posts from Addgene 1) Twitter – Get your voice out there Here’s one thing...involved in education advocacy non-profits like Phipps Conservatory or the Brilliant Club and share your...Radio, WIRED, Scientific American or National Geographic. Similarly, media fellowships from the British...

  13. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...in Streptomyces (Cobb et al., 2015). pCRISPomyces-1 includes Cas9, a tracrRNA, and a CRISPR array, while...INTEGRATE consists of four major components including (1) a CRISPR RNA, (2) four proteins forming the QCascade...identified the Cas9 orthologue from Streptococcus thermophilus, Cas9sth1 as a robust and highly active enzyme... al., 2014). These could be genes, specific polymorphisms, including antibiotic resistance genes. This...

  14. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...to western blotting  In a western, proteins are: (1) separated by size, (2) transferred to a membrane,...  (3) detected using antibodies.   Figure 1: Overview of the Western blot process. The sample,...sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein...

  15. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...in online patient/caregiver communities  Table 1: Examples of defining your audience for science communication...ethnic background, patient or caregiver status, geographic location, sense of humor, media consumed… really...

  17. Finding Your Science Policy Path

    Type
    Blog Post
    ...relations, advocacy, and writing legislation (see Figure 1 for career map). The beautiful part of science policy...about science policy as a profession. Figure 1: Science policy can lead you to many paths! For a ...approval for a new product or a well-endowed philanthropy who can shift the funding landscape to focus...

  18. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ... such a popular model organism? Zebrafish (Figure 1) were initially recognized as a useful vertebrate ...approach that exploits the bacteriophage integrase phiC31. The popular UAS/Gal4 and Cre/Lox gene regulatory...2018): 967-968. PubMed PMID: 29853675. Keller, Philipp J., et al. "Reconstruction of zebrafish early embryonic...

  19. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ... difficult to modify in the past.   Figure 1: Identification and enrichment use of XMAS-TREE. a...DNA of interest, and cell extract.    Graphical Abstract: Schematic representation of cell-free...

  20. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...variants. This resulted in the construction of redCALWY-1, a red-shifted FRET sensor for zinc using variants... his calcium indicators can be used in mouse, Drosophila, C. elegans, and zebrafish – essentially all ...
Showing: 161 - 180 of 208 results