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  2. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...In addition the transfection method (i.e. polyethyleneimine, calcium phosphate or liposomal methods), ...centrifugation on a sucrose gradient, spin columns, and polyethylene glycol (PEG) precipitation. All of these methods...

  3. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...such as the serotonin 3 receptor, will promote depolarization in neurons. Alternatively, combining with the...such as the glycine receptor, will promote hyperpolarization. PSAM ligands The advantage of PSAMs lies ...

  4. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...). mAbs and rAbs have higher homogeneity than polyclonal antibodies because of their immunoglobin component...circumvents the batch-to-batch variability found in polyclonal antibodies, creating a potentially unlimited ...

  5. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...and Bester 2021). For example, inserting a polyadenylation signal downstream of the transcription start...fragment. (D) Knock-in: insertion of a synthetic polyadenylation (spA) signal. Figure and caption adapted from...

  6. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...can influence the transcription of genes by RNA polymerase II, or by the addition of methyl- groups to the...9 methylation, while the K27 reporter uses the polycomb chromodomain to recognise lysine 27 methylation...

  7. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...conference If the conference has a social media policy, this should be on their website or the program...program booklet. Make sure you’re familiar with the policies before posting something on social media. If someone...

  8. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ... myidp.sciencecareers.org mentioned museums. Indianapolis is home to one of "The world’s biggest and best...children’s museum", The Children's Museum of Indianapolis. I drove passed these dinosaurs every day. They...

  9. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...single mRNA. IRES was originally discovered in poliovirus RNA, where it promotes translation of the viral...eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Pelletier et al (Nature. 1988 Jul 28;334...

  10. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...allows for the following to occur: A) Multiple polyclonal secondary IgGs can bind to different epitopes... Reagent Pros Cons Antibodies Bivalent Polyclonal Can be labeled with multiple dyes Commercially...

  12. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...relevant parties, and the company at large, by polling and informal communications we made a decision ...scientists often prefer data. At Addgene we rely on polls and surveys. A mix of closed (multiple choice/binary...

  13. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...While monoclonal and polyclonal antibodies are readily available from several sources, fewer sources ...Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies Resources on addgene.org Addgene's...

  14. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...CREATE cap plasmid, loxP sites flank the downstream polyA sequence. When viruses produced from this plasmid...into an animal with cell-type-specific Cre, the polyA sequence is inverted only in the Cre-expressing ...

  17. Tetracycline Inducible Expression

    Type
    Collection
    ...Expression of your gene of interest (MCS with a β-globin poly A) & EGFP from a bidirectional tet-responsive promoter...

  18. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...applications, including detection of environmental pollutants, drug discovery, and disease diagnosis. As biosensors...electrochemical sensors for emerging environmental pollutants.” Front Chem. 2014; 2: 41. PubMed PMID: 25019067...

  19. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...where they are incorporated into the growing polypeptide chain. One part of the tRNA contains the anticodon... codon for every amino acid across an entire polypeptide. Many researchers have created codon-optimization...

  20. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...sample is first amplified using RPA (recombinase polymerase amplification) or reverse transcriptase (RT)-...as well as health-related single nucleotide polymorphisms (SNPs) from human saliva. SHERLOCKv2 provides...
Showing: 161 - 180 of 362 results