We narrowed to 1,006 results for: RING

...potential to transform neuroscience research by bringing the precision of molecular targeting to specific...

...requested criteria. Please consider using fewer filtering options. Browse all Optogenetics AAV *AAVrg = ...

...vectors undergo quality control, including AAV titering by probe-based droplet digital PCR, in vitro and...

...Hasan, M. T., Bujard, H., & Hillen, W. (2000). Exploring the sequence space for tetracycline-dependent ...

...requested criteria. Please consider using fewer filtering options. *AAVrg = retrograde serotype, produced...

...: they require switching between a two-ring purine and one-ring pyrimidine base. This is a far more complex... the side groups of DNA bases and leave the core ring structure intact, allowing them to make 4 out of...backbone, making them efficient and precise tools for altering DNA sequences. These first base editors were both...researchers in the Keith Joung lab, who were engineering an ABE to reduce its tendency to make off-target...sites. To explore this further, the team started engineering a CBE to be worse at its original job. They ...glycosylase inhibitor (UGI) components, which inhibit BER during cytosine-to-uracil editing, and instead introduced...GBE editing but not CBE editing, maybe because repairing a GBE-derived abasic site requires more cellular...

...underside of the “umbrella” of A. victoria there is a ring that glows with a faint green light. To investigate...

...accessible and easier to share. As we prepare to ring in the New Year, let's make a toast to open science...those of us at Addgene believe in the power of sharing, and especially in keeping the products of science...

... position. Moving one position over in the sugar ring, a popular stabilizing modification is 3’ phosphorothioate...

...give to your questions. If one of their answers rings true for you, go ahead and give a similar answer... to the hiring manager and others, if you have their emails. You can use notes you took during your interviews...Fielding questions can be hard during this talk since you’ll be sharing your screen and won’t be able ...2016 and then working four more years in academia. During my job hunt, I quickly noticed one of the biggest... and/or small groups, usually starting with the hiring manager. These interviews lasted for 30 minute ...a list of who will be interviewing you, ask the hiring manager or recruiter for this information. I found...presentation to your audience Start by asking the hiring manager or recruiter what presentation format they...

...a protein based on the affinity of the imidazole ring of histidine for metal ions such as Ni2+ or Co2+...the polyhistidine tag? The polyhistidine tag is a string of 6 or more histidine residues expressed in frame...step to remove the tag in order to prevent it interfering with protein function. In contrast, the polyhistidine...using these more selective substrates along with stringent wash conditions.   Find polyhistidine tag plasmids...Sometimes a tag placed on one end will be blocked during protein folding, in which case you should try tagging...neither placement works, consider purifying under denaturing conditions. Unlike other affinity purification...). In some cases, proteins can be bound under denaturing conditions to expose the tag and then refolded...

...Stephen, Andrew G., et al. "Dragging ras back in the ring." Cancer cell 25.3 (2014): 272-281. PubMed PMID:...computer modeling have enabled researchers to begin discovering and designing molecules that are showing some...

...discovered that incorporation of four-membered azetidine rings could substantially enhance the brightness and photostability...

...actually has chimes outside of our break room. A ring of the chimes means something fun is going on and...in a unique environment where, in addition to furthering our mission by hard work, we genuinely enjoy ... that you don’t work for Addgene (though we are hiring!) but to encourage everyone to make an effort to... the power of simple word of mouth - be sure to bring up your event in casual conversation to get people...

...to help students during an interview. When they were instructed to use the three-ring binder as a starting...transferable skills learned while working in the lab and offering advice on areas where you might need some help...creativity is absolutely a transferable skill developed during PhD training. Creativity is required to develop...Whether it’s finding the source of contamination or figuring out why the lab is so cold, we’ve all had to think... need ahead of time and mute your notifications during. (Boyes, 2019) If you’ve taken the time to do this... assure that all participants feel comfortable sharing their ideas? And how do we give people the opportunity... The purpose of this form was to encourage the sharing of ideas, not to give people more work. Identify...

...camera lens without having to physically rotate the rings around the lens. It allowed me to focus on the subject...constraints given. And there were many constraints during the COVID-19 pandemic. The Pandemic Pivot Back...Even Blugene is practicing social distancing and wearing their mask. But while the camera is here, the operator...chair not watching their actors live on set, but peering at a 7-inch wireless monitor. Wireless video technology...control, and extra hand sanitizer.    Cycle 4: Venturing into the Lab Design: The lab is the beating heart...heart of Addgene. It’s why we exist, storing plasmids, and distributing them to labs that need them. The...projects lined up, some that started production during the middle of lockdown, and some that will spin...

...spent hours in the biosafety cabinet with cloning rings or following labor-intensive limiting dilution protocols...is used for a variety of applications such as measuring target protein expression levels, assessing post-translational...uses 2 or 3 different fluorescent colors each measuring a different target. Flow cytometers however are...methanol, fix and permeabilize in a single step by denaturing proteins and dissolving lipids including those...specialized components that can divert a subset of cells. During FACS, the sample stream oscillates to generate ...be targeted with specific fluorescent antibodies during the flow cytometry experiment and will appear as...of yellow and orange from FITC and that from PE. During compensation the signal arising from a specific...

... Protocols Pouring LB Agar Plates Pouring LB Agar Plates You may also like......solidifies in the bottle while you’re pouring, you should stop pouring and re-make the gel-mix. If you’re... autoclave tape. The autoclave tape will darken during the autoclave process if your sample has spent ...in the autoclave, you should prepare your plate pouring station: Find an empty section of lab bench with... the flame just to the side of where you’ll be pouring your plates - be sure to leave room for your bottle...appropriate antibiotics, and a section for active pouring. Prepare your antibiotics. Prior to adding your...1000x stock solution. For example: If you’ll be preparing plates with a final concentration of 100 ug/mL...

... Protocols Fluorescence Titering Assay Fluorescence Titering Assay for Lentivirus You may also...cells and debris. Lentiviral titer can decrease during cycles of freeze-thaw. If you are freezing and ...consider wells with less than 40% fluorescent cells. Titering methods assume one integration event per cell....

... Protocols Colony Formation Titering Assay Colony Formation Titering Assay for Lentivirus You may also...luer-lock filter, Corning 431229 10 mL luer-lock syringe, BD BD300912 0.45 μm polyethersulfone filter, Nalgene...added at the time of viral transduction, and not during the cell seeding step. Procedure Before beginning...cells and debris. Lentiviral titer can decrease during cycles of freeze-thaw. If you are freezing and ...