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Showing: 161 - 180 of 1010 results
  1. Top 10 Open Science Developments of 2013

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    Blog Post
    ...accessible and easier to share. As we prepare to ring in the New Year, let's make a toast to open science...those of us at Addgene believe in the power of sharing, and especially in keeping the products of science...
  2. CRISPR Plasmids - Plants

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    Collection
    ...like KRAB, can knock down gene expression by interfering with transcription. Design your gRNA to target...
  3. Control AAV Preps

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    Collection
    ...requested criteria. Please consider using fewer filtering options. Browse all Control AAV *AAVrg = retrograde...
  4. Lentivirus Plasmids

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    Collection
    ...Addgene's Viral Service Virus Biosafety Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries...
  5. Brain Armamentarium

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    Collection
    ...potential to transform neuroscience research by bringing the precision of molecular targeting to specific...
  6. Optogenetics AAV Preps

    Type
    Collection
    ...requested criteria. Please consider using fewer filtering options. Browse all Optogenetics AAV *AAVrg = ...
  7. Caltech Systemic Capsids

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    Collection
    ...vectors undergo quality control, including AAV titering by probe-based droplet digital PCR, in vitro and...
  8. Biosensor AAV Preps

    Type
    Collection
    ...requested criteria. Please consider using fewer filtering options. *AAVrg = retrograde serotype, produced...
  9. Genetic Code Expansion

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    Collection
    ...tyrosyl-tRNA synthetase M. jannaschii azobenzene-bearing uAAs Bacterial TAG Miriam Amiram 188537 pSerOTS-C1...
  10. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...give to your questions. If one of their answers rings true for you, go ahead and give a similar answer... to the hiring manager and others, if you have their emails. You can use notes you took during your interviews...Fielding questions can be hard during this talk since you’ll be sharing your screen and won’t be able ...2016 and then working four more years in academia. During my job hunt, I quickly noticed one of the biggest... and/or small groups, usually starting with the hiring manager. These interviews lasted for 30 minute ...a list of who will be interviewing you, ask the hiring manager or recruiter for this information. I found...presentation to your audience Start by asking the hiring manager or recruiter what presentation format they...
  11. To Each HIS Own

    Type
    Blog Post
    ...a protein based on the affinity of the imidazole ring of histidine for metal ions such as Ni2+ or Co2+...the polyhistidine tag? The polyhistidine tag is a string of 6 or more histidine residues expressed in frame...step to remove the tag in order to prevent it interfering with protein function. In contrast, the polyhistidine...using these more selective substrates along with stringent wash conditions.   Find polyhistidine tag plasmids...Sometimes a tag placed on one end will be blocked during protein folding, in which case you should try tagging...neither placement works, consider purifying under denaturing conditions. Unlike other affinity purification...). In some cases, proteins can be bound under denaturing conditions to expose the tag and then refolded...
  12. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...actually has chimes outside of our break room. A ring of the chimes means something fun is going on and...in a unique environment where, in addition to furthering our mission by hard work, we genuinely enjoy ... that you don’t work for Addgene (though we are hiring!) but to encourage everyone to make an effort to... the power of simple word of mouth - be sure to bring up your event in casual conversation to get people...
Showing: 161 - 180 of 1010 results