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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 1,006 results for: RING;

Showing: 161 - 180 of 1006 results

  1. Microbiology Resources

    Type
    Collection
    ...Read our CRISPR Methods for Bacterial Genome Engineering blog post to see how Addgene depositors are making...

  2. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...like KRAB, can knock down gene expression by interfering with transcription. Design your gRNA to target...

  3. TALEN Guide

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    Collection
    ...Nucleic Acids Research article that came out in the spring of 2011, the groups describe a set of customized...

  4. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...fluorescence microscopy by exciting the donor and measuring the emission of the acceptor. For a given donor-acceptor...

  6. CRISPR Plasmids - Plants

    Type
    Collection
    ...like KRAB, can knock down gene expression by interfering with transcription. Design your gRNA to target...

  7. Control AAV Preps

    Type
    Collection
    ...requested criteria. Please consider using fewer filtering options. Browse all Control AAV *AAVrg = retrograde...

  9. Brain Armamentarium

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    Collection
    ...potential to transform neuroscience research by bringing the precision of molecular targeting to specific...

  10. Lentivirus Plasmids

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    Collection
    ...Addgene's Viral Service Virus Biosafety Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries...

  13. Optogenetics AAV Preps

    Type
    Collection
    ...requested criteria. Please consider using fewer filtering options. Browse all Optogenetics AAV *AAVrg = ...

  14. Caltech Systemic Capsids

    Type
    Collection
    ...vectors undergo quality control, including AAV titering by probe-based droplet digital PCR, in vitro and...

  15. Biosensor AAV Preps

    Type
    Collection
    ...requested criteria. Please consider using fewer filtering options. *AAVrg = retrograde serotype, produced...

  16. Genetic Code Expansion

    Type
    Collection
    ...tyrosyl-tRNA synthetase M. jannaschii azobenzene-bearing uAAs Bacterial TAG Miriam Amiram 188537 pSerOTS-C1...

  19. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...give to your questions. If one of their answers rings true for you, go ahead and give a similar answer... to the hiring manager and others, if you have their emails. You can use notes you took during your interviews...Fielding questions can be hard during this talk since you’ll be sharing your screen and won’t be able ...2016 and then working four more years in academia. During my job hunt, I quickly noticed one of the biggest... and/or small groups, usually starting with the hiring manager. These interviews lasted for 30 minute ...a list of who will be interviewing you, ask the hiring manager or recruiter for this information. I found...presentation to your audience Start by asking the hiring manager or recruiter what presentation format they...

  20. To Each HIS Own

    Type
    Blog Post
    ...a protein based on the affinity of the imidazole ring of histidine for metal ions such as Ni2+ or Co2+...the polyhistidine tag? The polyhistidine tag is a string of 6 or more histidine residues expressed in frame...step to remove the tag in order to prevent it interfering with protein function. In contrast, the polyhistidine...using these more selective substrates along with stringent wash conditions.   Find polyhistidine tag plasmids...Sometimes a tag placed on one end will be blocked during protein folding, in which case you should try tagging...neither placement works, consider purifying under denaturing conditions. Unlike other affinity purification...). In some cases, proteins can be bound under denaturing conditions to expose the tag and then refolded...
Showing: 161 - 180 of 1006 results