We narrowed to 665 results for: SIM;

... to a Perfect Science Talk." 4. The chalk talk, simple in principle The first question I usually get about...focus of your first grant? (1-2 sentences) Simply stated, what are your aims? You could directly ...official aims, but I suggest distilling them into simple goals/outcomes. This isn't the time to go into ...in the knowledge that you have given everyone a simple plain language map of where you are going and what...

...techniques and strategies one can employ beyond simply incubating cells with single-fluorophore-conjugated... B cells, and dendritic cells. In this case, we simply need to label the lineage markers of all cell types...selected color will not be CD8+ T cells and we can simply “dump” them by gating on the negative population... new methods of target visualization beyond the simple surface labeling. Let’s briefly walk through the...

...that strongly binds the glycoprotein, avidin or a similar protein, streptavidin. This strong interaction ...Thus to capture protein complexes, scientists can simply express a bait protein fused to BirA* in cells ...identify additional interacting proteins in one simple experiment. PCA is an in vivo technique where two... and Paul A. Khavari. "Research Techniques Made Simple: Emerging Methods to Elucidate Protein Interactions...

...next-generation sequencing techniques to answer similar experimental questions (see below). Importantly...location of a given nucleotide, a researcher (or a simple computer program) can then reproduce the DNA sequence...generation DNA sequencing machines (9). Today, similar technologies, sometimes referred to as sequence...Kathleen, and Daniel Nathans. "Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus...

...because of its relative simplicity as a gene editing system. Despite that simplicity, its accessibility outside... worse. To better understand this transfer, the Simon Grelet Lab developed MitoTRACER, a retroviral-based...the blue-light photoswitching issue). Although similar in brightness to jRGECO1a and FRCaMP, FRCaMPi had...

...cassette (Dickinson et al., 2015) made this step as simple as heat-shocking to induce the expression of a ...my initial direct screening efforts, which were similar to the approach detailed by Paix et al. Although...Addgene. In 2019, Farboud et al. described another similar co-conversion marker (repair of a zen-4(cle10ts...choosing. Tools such as SapTrap Builder greatly simplifies design of these constructs, allowing novice researchers...

...reason most people enter postdoctoral training. Similarly, there are several factors to consider when selecting... week planning an experiment, even a relatively simple experiment. However, by taking this time, you can...result? Is there a tractable question or are you simply describing a phenomenon? Exploration can be important...Plus, presentations benefit from practice. If you simply recycle slides from the last time you gave a presentation...

...using the csRNP method is as simple as doing routine PCR assays. Here is a simple to-do-list to follow when...spectrometry to identify the interacting proteins. Similarly, in ChIP-seq assays, chromatin fragments that ...successfully knock-in non-expressed genes with similar efficiency, Dewari et al. 2018). Using the first...

...Fluorescent proteins (FPs) offer scientists a simple yet powerful way to tag cellular proteins and investigate...fluorescence when illuminated with blue light (1). Similar to PA-GFP, scientists later developed red fluorescent...photoactivatable fluorescent protein for your experiment Similar to performing experiments with standard FPs, before...live imaging of proteins, organelles, and cells. Similar to FPs, PA-FPs allow for non-invasive labelling...

.... To start the AAV packaged on request process, simply navigate to your plasmid of interest. If it’s eligible...cargo you requested at the quality you expect. Similar to our on-demand viral service, we expect titers...There are no quotes or special processes involved; simply request your plasmid of interest in your desired...

... used as the first letter of a plasmid name and simply denotes that the object is a ‘plasmid’. “p” is ... empty backbone name in your plasmid name. This simple piece of information can often convey many important...pBACKBONE-Flag-hGene-EGFP or pBACKBONE-Flag-hGene(wt)-EGFP These simple rules allow any scientist to know what is in a ...

...edited strand. The first iteration of prime editor simply fused the wild-type Moloney Murine Leukemia Virus...or exceed PEmax editing efficiencies for short, simple edits. PE6c and PE6d further evolved the Tf1 and...pegRNAs, but combining PE7 with epegRNAs resulted in similar or reduced editing efficiencies, likely because...Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt...

...control over neuronal activity. While they serve similar purposes, both techniques offer researchers different...resolution as receptors can be turned on and off simply with the presence or absence of the correct wavelength...flexible as it relies on designer ligands that can be simply introduced by injection. The main challenge in ...

... the Cellosaurus STR similarity search tool (CLASTR) to find cells lines similar to your STR profiles ...CLASTR results by minimal number of markers and similarity score match. This tool’s algorithm can be adjusted...

...and cell lines. Although ChIP-seq is a relatively simple and robust protocol it does have limitations. The...change offers a significant improvement and simplification of the original technique, specifically in ...CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids for isolating...

... DA in live animals (Patriarchi et al., 2018). Similar to calcium and glutamate sensors, the new dLight1...s lab has created the GRABDA sensor based on a similar strategy: a GFP variant bound to a DA receptor,...cyclase in caudate nucleus of rat brain, and its similarity to the “dopamine receptor”." Proceedings of the...

...Drawings is similar to using PowerPoint to draw figures. It’s part of Google Drive so it has a similar interface...figures quickly. Inkscape Inkscape is the most similar to Adobe Illustrator out of the three options for...

...Interspaced Short Palindromic Repeats. That contrast of simplicity and complexity is reflected in the biology, too...into a single strand that folds back on itself, simplifying the CRISPR-Cas9 system for laboratory applications...post on the structural biology of Cas9. Want a simpler guide to keep at your desk? We’ve got a CRISPR ...

...equipment. Reporters for studying gene expression have similar drawbacks, often requiring the destruction of plants... events and am currently planning to use it to simplify my work with an Arabidopsis mutant that can’t ...unfamiliar with these mutant plants. Using RUBY to simplify the identification of these mutants will speed...

...resistant to vaccine serum responses. This result was similar to studies that used live virus (Cele et al., 2021... shocked [with these results] because we were simulating the live virus experiment,” says Balazs. “But...neutralization rates of B.1.351 pseudovirus was similar to that of pseudoviruses representing a bat-derived...