We narrowed to 238 results for: SPL

...presentation describing the technology. Then we split into pairs to actually try out the kit itself. This...

...very robust, with an eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki...

...below. Digital PCR Digital PCR (dPCR) involves splitting up a sample into thousands of physically isolated...

...choose, you’ll need to reserve a small aliquot, split into two parts to (1) run a DNA gel to confirm fragment...

...David Liu Lab similarly described xCas9, which displayed the ability to target multiple PAM sequences (...

...work, they have found at least two variants that display increased neuronal expression in the brain while...

...its native endonuclease activity degrades the displaced strand.   Figure 1: EvolvR consists of ...

...is also common. Overlap extension PCR is used to splice together two PCR amplicons based on sequence homology...

...the nerve to do what I did. Until that panicked, split-second decision, I had always thought I had an obligation...

...same time period. For ease of calculations*, let’s split the difference and put scientific inflation at… ...

...top of photobleaching, fluorescent proteins may display reversible intensity changes (Shaner et al, 2008...

...cleavage and 2). cell toxicity. Cas7-11 does not display collateral RNA cleavage and is significantly less...

...the Uppalapati and Woolley lab developed a phage display technique using small scaffold proteins to identify...

...SpCas9 N692A/M694A/Q695A/H698A). This new variant displays comparable on-target activity and similar or decreased...

... reliability. Notably, the SpRY variant didn’t display higher off-target effects than SpCas9, a pleasant...

...or face shields are required whenever there’s a splash or spray risk in BSL-1. In BSL-2 labs, eye protection...

...direct evolution, capsid shuffling, and peptide displays. These capsids will be selected both for their...

...reduced. Third generation packaging systems further split the viral genome by removing the viral Rev gene ...

...purposes, retroviral vectors have been developed into ‘split-vector systems’ by separating viral genes and transgene...

...chicken beta actin promoter, and rabbit beta-globin splice acceptor, contains sequence that is over 80% GC...