We narrowed to 237 results for: SPL

1. Luminescent Imaging with Nano-lanterns

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   Blog Post

   ...very robust, with an eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki...

2. Screening for Successful Genome Editing with Digital PCR

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   ...below. Digital PCR Digital PCR (dPCR) involves splitting up a sample into thousands of physically isolated...

3. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   ...is also common. Overlap extension PCR is used to splice together two PCR amplicons based on sequence homology...

4. CRISPR Pooled gRNA Libraries

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   ...Knockout Human Olzmann 3rd ~10 13,920 Human lncRNA Splicing-targeting CRISPR Library 119977 Knockout Human...

5. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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   ...the Uppalapati and Woolley lab developed a phage display technique using small scaffold proteins to identify...

6. Transferable Skills Guide: Public Speaking

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   ...the nerve to do what I did. Until that panicked, split-second decision, I had always thought I had an obligation...

7. The time and cost required to make a plasmid

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   ...same time period. For ease of calculations*, let’s split the difference and put scientific inflation at… ...

8. CRISPR 101: Cas9 vs. The Other Cas(s)

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   ...cleavage and 2). cell toxicity. Cas7-11 does not display collateral RNA cleavage and is significantly less...

9. Choosing the B(right)est Fluorescent Protein: Photostability

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   ...top of photobleaching, fluorescent proteins may display reversible intensity changes (Shaner et al, 2008...

10. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    ...SpCas9 N692A/M694A/Q695A/H698A). This new variant displays comparable on-target activity and similar or decreased...

11. Tetracycline Inducible Expression

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    ...cortical neurons Michael Ward 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA Helper virus for monosynaptic tracing...

12. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    ...no detectable editing. Additionally, off-target spleen editing was not detected and Nanoblade injection...

13. Progress Towards a PAM-Free CRISPR

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    ... reliability. Notably, the SpRY variant didn’t display higher off-target effects than SpCas9, a pleasant...

14. Overcoming the Challenges of Lentiviral Production

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    ...reduced. Third generation packaging systems further split the viral genome by removing the viral Rev gene ...

15. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...purposes, retroviral vectors have been developed into ‘split-vector systems’ by separating viral genes and transgene...

16. Viral Vectors 101: Virus Safety

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    ...or face shields are required whenever there’s a splash or spray risk in BSL-1. In BSL-2 labs, eye protection...

17. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    ...direct evolution, capsid shuffling, and peptide displays. These capsids will be selected both for their...

18. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...chicken beta actin promoter, and rabbit beta-globin splice acceptor, contains sequence that is over 80% GC...

19. Cpf1 Update: Comparison to Cas9 and NgAgo

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    Blog Post

    ... cleavage. Now, two new studies show that Cpf1 displays lower off-target editing than Cas9, confirming...

20. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    ...happens often because the HDR inserted sequence splits up the guide sequence.) If neither of the above...