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  1. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...incorporated at the termini of the crRNA molecule (usually 1-3 of the first 3 bps are modified) for optimal...RNA in your targeting experiments allows you to visualize transfection efficiency and even track localization...

  3. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...to your primary antibody. If you are trying to visualize the precise location of a protein in a tissue ...secondaries:conjugates complex. If you are trying to visualize a protein in low abundance on a Western blot, ...

  4. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...secondary antibodies may be indicated on occasion, usually after other signal amplification techniques have...on the signaling molecule solely asmeans of visualization. This means that if all the primary antibodies...

  5. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...independently from its host’s chromosome. Although we usually think of bacteria when we think of plasmids, both...manage this with plasmids that allow them to eat unusual substrates that other organisms can’t use. Other...

  6. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...ubiquitous background signal that makes it hard to visualize the precise location of the protein or structure... solution because it allows for tagging and visualization of specific, individual proteins under endogenous...

  7. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...systems use 3 plasmids; an envelope plasmid that usually encodes VSV-G, a packaging plasmid containing structural...gene are transfected into a packaging cell line, usually a derivative of 293T. Packaging cells are incubated...

  8. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...rabies virus (RABV) can be used in the brain to visualize how neurons are connected. Viral Vectors as ...neurotropic viruses emerged as powerful tools for visualizing neural connectivity (Nassi et al. 2015). The ...

  13. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ... Physical versus functional titer Viral titers usually come in two flavors, physical or functional. Physical... amount of viral particles in a sample and are usually based on the presence of a viral protein, such ...

  14. Common Injection Routes in Mice

    Type
    Blog Post
    ...advanced and finally, to expert.   Figure 1. Visual representation of common injection routes in mice...straightforward, as it targets large muscle groups (usually the thigh), though it is important to avoid injection...

  15. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...protein of interest is untagged and functions per the usual. In the presence of a specific protease inhibitor... initially developed as an affinity tag for visualization of proteins of interest. A fluorescent Halo ...

  16. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...sure your cells don’t stay out too long. Cells usually have their comfort zone around 37 °C and 5% CO2...to pH. Anything between orange and light orange usually indicates that the cells have grown enough, are...

  17. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...biotechnological, and medical applications. Researches can visualize proteins in vivo by tagging them with fluorescent... of use, it is important to note that bacteria usually cannot produce functional multi-domain mammalian...

  18. PhD Applications After COVID

    Type
    Blog Post
    ...the first steps in the application process and usually occurs in the few months leading up to applications...such-and-such university State how you found their lab (usually through the university website or, even better,...

  19. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...students in academia. Getting a drug to the market usually takes over a billion dollars and at least a decade...off as an industry postdoc since your funding is usually separate from a project’s or a team’s, it is possible...

  20. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...are smaller antibodies.They are derived from an unusual type of IgG antibody called a heavy chain antibody...fluorophore conjugates are used, but these are usually more expensive than unconjugated primary antibodies...
Showing: 161 - 180 of 410 results