Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 424 results for: SUA

Showing: 161 - 180 of 424 results

  1. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...systems use 3 plasmids; an envelope plasmid that usually encodes VSV-G, a packaging plasmid containing structural...gene are transfected into a packaging cell line, usually a derivative of 293T. Packaging cells are incubated...

  2. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...rabies virus (RABV) can be used in the brain to visualize how neurons are connected. Viral Vectors as ...neurotropic viruses emerged as powerful tools for visualizing neural connectivity (Nassi et al. 2015). The ...

  4. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...ubiquitous background signal that makes it hard to visualize the precise location of the protein or structure... solution because it allows for tagging and visualization of specific, individual proteins under endogenous...

  5. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...independently from its host’s chromosome. Although we usually think of bacteria when we think of plasmids, both...manage this with plasmids that allow them to eat unusual substrates that other organisms can’t use. Other...

  9. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ... Physical versus functional titer Viral titers usually come in two flavors, physical or functional. Physical... amount of viral particles in a sample and are usually based on the presence of a viral protein, such ...

  10. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...sure your cells don’t stay out too long. Cells usually have their comfort zone around 37 °C and 5% CO2...to pH. Anything between orange and light orange usually indicates that the cells have grown enough, are...

  11. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...biotechnological, and medical applications. Researches can visualize proteins in vivo by tagging them with fluorescent... of use, it is important to note that bacteria usually cannot produce functional multi-domain mammalian...

  12. Common Injection Routes in Mice

    Type
    Blog Post
    ...advanced and finally, to expert.   Figure 1. Visual representation of common injection routes in mice...straightforward, as it targets large muscle groups (usually the thigh), though it is important to avoid injection...

  13. PhD Applications After COVID

    Type
    Blog Post
    ...the first steps in the application process and usually occurs in the few months leading up to applications...such-and-such university State how you found their lab (usually through the university website or, even better,...

  14. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...characterized and deposited a variety of constructs for visualizing cellular components (nucleus, endosomes, lysosome...and an epitope tag (ALFA-tag, or At) for easy visualization. A cleavage site for endogenous BACE1 protease...

  15. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...protein of interest is untagged and functions per the usual. In the presence of a specific protease inhibitor...developed by Promega as an affinity tag for visualization of proteins of interest. A fluorescent Halo ...

  16. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...students in academia. Getting a drug to the market usually takes over a billion dollars and at least a decade...off as an industry postdoc since your funding is usually separate from a project’s or a team’s, it is possible...

  17. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

  18. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...are smaller antibodies.They are derived from an unusual type of IgG antibody called a heavy chain antibody...fluorophore conjugates are used, but these are usually more expensive than unconjugated primary antibodies...

  19. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...immunofluorescence (IF) - a common method for visualizing molecules of interest within a cell or tissue...your sample type. Starting with a protocol that usually works in your system and then optimizing from there...

  20. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...responsible for each figure. Others still are more casual with a single presenter summarizing the paper’s...could be solicited via multiple avenues including casual request via email, in person, or instant messaging...
Showing: 161 - 180 of 424 results