We narrowed to 579 results for: SUB

...CamR gene. Screening for surviving colonies and subsequent rounds of evolution and engineering gave rise...TadA*) that satisfactorily accepts DNA as its substrate (see figure 2).          The artificially evolved...which is treated as ‘G’ by cellular polymerases. Subsequently, an original genomic A-T base pair is converted...stable transformation, regenerated plants may be subjected to genotyping. If successful base editing causes...

...Every few months, we highlight a subset of the new plasmids, antibodies, and viral preps in the repository...compartments. They showed APEX2 can use an orthogonal substrate, alkyne-phenol, to label proteins with an alkyne...development in and of itself. After adding one enzyme’s substrate, then the other, a dual-enrichment and mass spectrometry...Andrews, N.P., et al. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

...can be used to introduce specific nucleotide substitutions (or deletions) in a tailored manner. The approach...perform alanine scanning mutagenesis or targeted substitution of key residues), and in SNP analysis (to introduce...site through mutagenesis vastly facilitates the subsequent process of screening for succesfully mutated ... difficult to sequence), it may be easier to subsequently clone a smaller region (containing the specific...

...functional luciferase enzyme (Figure 1). Addition of substrate generates a proportionate luminescent signal, ...precise quantification with with nothing more than substrate addition and a simple add-mix-measure method. ...to the binding sites and various isoforms led to subtle changes in co-receptor dynamics, including NanoBRET...sea shrimp utilizing a novel imidazopyrazinone substrate. ACS Chemical Biology, 7(11), 1848–1857. https...

...group as well as evolution of the coelenterzine substrate to the more stable yet brighter furimazine by ...i.p. or i.v. administration of the furimazine substrate for NanoLuc luciferase. Both GpNLuc and OgNLuc...multiple cellular events such as the dynamics of subcellular structures and gene expression. You must see ...sea shrimp utilizing a novel imidazopyrazinone substrate. ACS Chem Biol 7, 1848-57. PubMed PMID: 22894855...

...Anyone can submit data from their CLOVER lab or Addgene AAV experiments to the Data Hub. Submit your data...Interested? You can learn more here - and remember, submissions are due Nov. 21!   ...

...example, cell sorting. The separated cells are subsequently sequenced by next generation sequencing to identify...approach is to use smaller libraries targeting subsets of genes. With such an approach, you lose the unbiased...stimulation would then be separated by sorting, and subsequently sequenced to identify which signaling molecules...Inc for this purpose.  The gRNA oligos are subsequently cloned into your vector of choice using the ...

...as they cast their ballots for their favorite submission. So without any further ado, we present to you...receives an honorable mention for their second submission.    Our Cultured Warrior (made by Nate using ...from Lab Support at the John Innes Centre, and submitted by the Lab Support Team at the Centre.     Lab...

...of GFP, its brighter mutant S65T-GFP, and the subsequent biochemical characterization, has enabled scientists...mitochondrial targeting sequence of cytochrome c oxidase subunit VIII, so that they can be imported to the mitochondrial...Chlamydomonas noctigama (CnChR1), Chloromonas subdivisa (CsChR), Chloromonas oogama (CoChR), and Scherffelia...electron microscopy (EM) tag providing superior subcellular localization of tagged proteins compared to traditional...proteins of interest for EM imaging, or targeted to subcellular regions or protein complexes for proteomic mapping...

... are you’ve used a GFP derivative to conduct subcellular localization studies or make a reporter construct...et al. noticed that GFP had turned red. They subsequently confirmed that purified GFP could be imaged ...Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis JS...

...NanoLuc, require injection of substrate to produce bioluminescence. Substrate pharmacokinetics and biodistribution...gastrointestinal tract. Require Administration of Substrate: Some luciferase reporters, such as Renilla luciferase...

... tested the ability of GeoCas9 RNPs to cleave substrate when the reaction was carried out at elevated ... in diluted human plasma for 8 hours at 37°C. Substrate was then added and enzyme activity measured. SpCas9...reaction buffer for 8 hours at either 37 °C or 4 °C. Substrate was then added and cleavage product analyzed on...

...with smaller nick distances. Using D10A, they subsequently showed that editing efficiency is very low when...Cas9 with the left gRNA across the window. In a subsequent experiment, they were also able to introduce ...and Yan next examined a situation thought to be suboptimal for HDR. At the AAVS1 locus, the two nearest ...

...to silence or incite neuronal firing and study subsequent effects on behavior. The system is an especially...the lab expressed the LiGluR opsin in different subsets of spinal cord neurons using the Gal4/UAS system...blog posts Read blog posts about viral vectors Subscribe to the Addgene blog Resources on Addgene.org ...

...targeted to specific tissues, cell types or even subcellular regions of a neuron. Optogenetic approaches excel... excel at discrete cell or tissue-subregion targeting down to the limits of light delivery. Chemogenetic...DR If precise spatiotemporal control of a small subset of neurons is necessary, use optogenetics. If broad...

...total of eight evolutionarily conserved CRISPR subtypes comprised of more than 40 cas gene families have...grouped the systems into three distinct types and subtypes using polythetic classification based on evolutionary...system and are not translatable to the other CRISPR subtypes. Still, the large and dynamic range of CRISPR ...

...labs. This week we are pleased to announce the submission of the results of our first study which can be...PubMed PMID: 16528756. 2. Oh, Steve KW, et al. "Substantial overproduction of antibodies by applying osmotic...Amin Hajitou. "Clathrin-mediated endocytosis and subsequent endo-lysosomal trafficking of adeno-associated...

...interest in STEM subjects; we generally refer to them as the “interested public.” Subscribers to Scientific...Common ones include age range, familiarity with the subject, shared interests, socioeconomic and/or racial/...

... (Fig. 1). However, once thawed, there was a substantial reduction (from 7.4 to 5.0) in the pH of samples...periods, and measured the pH. We found that tubes subjected to a recovery period of at least 96 hours had ...infectivity of the virus. However, there was a substantial reduction in infectivity after 3 weeks of exposure...

...recombineering to create insertions, deletions, and substitutions - at the same efficiency as Gibson Assembly ...amplicons based on sequence homology, and the subsequent product is then digested and ligated into an ...nick repair is needed. Since the bacteria are subsequently grown at 37 °C, the recombineering plasmid is...