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We narrowed to 581 results for: SUB

Showing: 161 - 180 of 581 results

  1. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...synthesizing fluorophore fusions on their own. Subsequent advances in fluorescent proteins have replicated...from a sample. Chemical fluorophores can be substantially brighter and more photostable than fluorescent...reacts specifically and irreversibly with a small substrate ligand motif attached to a fluorophore (Figure...incorporation of four-membered azetidine rings could substantially enhance the brightness and photostability of...

  2. Using AAV for Neuronal Tracing

    Type
    Blog Post
    .... In the specific example of episodic memory, a subset of hippocampal neurons projects axons directly ...tracing has the added benefit of only labelling the subset of infected cells that also express Cre. Another...the ability to spread trans-synaptically on to subsequent neurons. AAV vectors are replication incompetent...incompetent and cannot readily be passed on to subsequent cells via replication like RABV or HSV. There are,...transsynaptic transfer of a protein, tetanus toxin, subsequent to its retrograde axonal transport. J Cell Biol...

  3. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...produce substances toxic to themselves? What are toxin-antitoxin systems? These toxic substances are part...inferes with DNA replication by binding the GyrA subunit of DNA gyrase and trapping it in the cleaved complex... in vitro without antibiotic selection. In a subcutaneous cancer model, the researchers showed that after...

  4. Hot Plasmids - November 2023

    Type
    Blog Post
    ...Every few months, we highlight a subset of the new plasmids, antibodies, and viral preps in the repository...compartments. They showed APEX2 can use an orthogonal substrate, alkyne-phenol, to label proteins with an alkyne...development in and of itself. After adding one enzyme’s substrate, then the other, a dual-enrichment and mass spectrometry...Andrews, N.P., et al. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

  5. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...CamR gene. Screening for surviving colonies and subsequent rounds of evolution and engineering gave rise...TadA*) that satisfactorily accepts DNA as its substrate (see figure 2).          The artificially evolved...which is treated as ‘G’ by cellular polymerases. Subsequently, an original genomic A-T base pair is converted...stable transformation, regenerated plants may be subjected to genotyping. If successful base editing causes...

  6. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...can be used to introduce specific nucleotide substitutions (or deletions) in a tailored manner. The approach...perform alanine scanning mutagenesis or targeted substitution of key residues), and in SNP analysis (to introduce...site through mutagenesis vastly facilitates the subsequent process of screening for succesfully mutated ... difficult to sequence), it may be easier to subsequently clone a smaller region (containing the specific...

  7. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...group as well as evolution of the coelenterzine substrate to the more stable yet brighter furimazine by ...i.p. or i.v. administration of the furimazine substrate for NanoLuc luciferase. Both GpNLuc and OgNLuc...multiple cellular events such as the dynamics of subcellular structures and gene expression. You must see ...sea shrimp utilizing a novel imidazopyrazinone substrate. ACS Chem Biol 7, 1848-57. PubMed PMID: 22894855...

  8. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...functional luciferase enzyme (Figure 1). Addition of substrate generates a proportionate luminescent signal, ...precise quantification with with nothing more than substrate addition and a simple add-mix-measure method. ...to the binding sites and various isoforms led to subtle changes in co-receptor dynamics, including NanoBRET...sea shrimp utilizing a novel imidazopyrazinone substrate. ACS Chemical Biology, 7(11), 1848–1857. https...

  9. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    ...Anyone can submit data from their CLOVER lab or Addgene AAV experiments to the Data Hub. Submit your data...Interested? You can learn more here - and remember, submissions are due Nov. 21!   ...

  10. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...example, cell sorting. The separated cells are subsequently sequenced by next generation sequencing to identify...approach is to use smaller libraries targeting subsets of genes. With such an approach, you lose the unbiased...stimulation would then be separated by sorting, and subsequently sequenced to identify which signaling molecules...Inc for this purpose.  The gRNA oligos are subsequently cloned into your vector of choice using the ...

  11. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...as they cast their ballots for their favorite submission. So without any further ado, we present to you...receives an honorable mention for their second submission.    Our Cultured Warrior (made by Nate using ...from Lab Support at the John Innes Centre, and submitted by the Lab Support Team at the Centre.     Lab...

  12. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...of GFP, its brighter mutant S65T-GFP, and the subsequent biochemical characterization, has enabled scientists...mitochondrial targeting sequence of cytochrome c oxidase subunit VIII, so that they can be imported to the mitochondrial...Chlamydomonas noctigama (CnChR1), Chloromonas subdivisa (CsChR), Chloromonas oogama (CoChR), and Scherffelia...electron microscopy (EM) tag providing superior subcellular localization of tagged proteins compared to traditional...proteins of interest for EM imaging, or targeted to subcellular regions or protein complexes for proteomic mapping...

  13. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ... are you’ve used a GFP derivative to conduct subcellular localization studies or make a reporter construct...et al. noticed that GFP had turned red. They subsequently confirmed that purified GFP could be imaged ...Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis JS...

  14. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...targeted to specific tissues, cell types or even subcellular regions of a neuron. Optogenetic approaches excel... excel at discrete cell or tissue-subregion targeting down to the limits of light delivery. Chemogenetic...DR If precise spatiotemporal control of a small subset of neurons is necessary, use optogenetics. If broad...

  15. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...to silence or incite neuronal firing and study subsequent effects on behavior. The system is an especially...the lab expressed the LiGluR opsin in different subsets of spinal cord neurons using the Gal4/UAS system...blog posts Read blog posts about viral vectors Subscribe to the Addgene blog Resources on Addgene.org ...

  17. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ...with smaller nick distances. Using D10A, they subsequently showed that editing efficiency is very low when...Cas9 with the left gRNA across the window. In a subsequent experiment, they were also able to introduce ...and Yan next examined a situation thought to be suboptimal for HDR. At the AAVS1 locus, the two nearest ...

  18. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ... tested the ability of GeoCas9 RNPs to cleave substrate when the reaction was carried out at elevated ... in diluted human plasma for 8 hours at 37°C. Substrate was then added and enzyme activity measured. SpCas9...reaction buffer for 8 hours at either 37 °C or 4 °C. Substrate was then added and cleavage product analyzed on...

  19. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...total of eight evolutionarily conserved CRISPR subtypes comprised of more than 40 cas gene families have...grouped the systems into three distinct types and subtypes using polythetic classification based on evolutionary...system and are not translatable to the other CRISPR subtypes. Still, the large and dynamic range of CRISPR ...

  20. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...interest in STEM subjects; we generally refer to them as the “interested public.” Subscribers to Scientific...Common ones include age range, familiarity with the subject, shared interests, socioeconomic and/or racial/...
Showing: 161 - 180 of 581 results