...way to develop and exercise your scientist skill set. If you dread the thought of writing a review, or...I was by no means an expert on this topic when I set out to write the review. That’s just to say that ... isn’t possible. So, define your scope from the onset. Perhaps you, a colleague, or your adviser was invited...

...cloning technique that will work well with a given set of resources, time, and experimental needs. Since...restriction and ligation cloning. It is also possible to set up the BP and LR reactions in the same tube, speeding... create an entry clone: Restriction cloning the inset into an attL-containing entry vector.   Pro...

...communicating ideas in a content-rich, easy-to-understand set of PowerPoint slides. What are some of the more challenging... biology or medicine you will bring an essential set of tools to a consulting team. Your case team will...

...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers...!). Then schedule a meeting to discuss the work, set expectations, answer any questions, and (most importantly...

...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers...doesn’t end after you get your first new job. To set yourself up for success in the career you hope to...

...that can be difficult to replicate in other work settings: The sense of being the first to discover something...academia provide a formal training regime and the right set of tools to help you do your job as well as possible... find the idea of not holding a pipette again upsetting, there are ways to get back to the bench during...

...will need to grow this initial stock and create a set of vials of ‘Master’ stock, which can be stored in...Master vial can then be thawed and used to produce a set of ‘working’ stocks of cells. Working stocks should...

...order to try and access the entire interactome of a set of proteins at once.  However, as Y2H screens are...positive and false negatives, these experiments must be set up, conducted and analysed with care to get reliable...entire proteome of a particular cell type, or a subset based on a cellular compartment or growth stage... as a good positive control to ensure that your setup works. The last thing you want is to run your lovely...

...plenty of events that included a simple corn hole set and a cooler. Chances are one of your colleagues ...especially true. If your event requires a lot of set up or cleanup, ask for volunteers to help out. Delegate...

...make sense of it, with their decision to publish setting the clock for others to dive in. The real cost ...usually condensed into a statistically evaluated set of values.  However, to evaluate the interpretation...anonymous request.  This means that supporting datasets must be logically annotated and arranged, as in...should be primarily responsible for storage.  If datasets are too large to transfer to a journal repository...peer-reviewed repository for “scientifically valuable” datasets. It likely won’t be long before other data repositories... move towards encouraging authors to integrate datasets with their resultant figures. Instead of providing...

...hoarding used tip boxes for a lab supply tree and set a challenge to my lab mates. They did not disappoint...

...Blog Series We have not one but two new blog series set to launch this summer! Summer SciComm Series From...

...scavenger hunts. In fact, they’ve been following a set of clues that has led them to their biggest discovery...

...is an integral part of any bacteriologist’s skill set. So when Dave Westenberg taught this concept in his...the streak plate challenge. Chromoproteins are a subset of the fluorescent protein family that give off...

... super family). To overcome these challenges, we set out to develop a hassle-free, highly efficient Cas9...eLife 2018;7:e35069 DOI: 10.7554/eLife.35069. How to set up csRNP knock-in experiments If your lab is new ...experiments, there is no need to worry at all. Setting up tag knock-in experiments using the csRNP method...

...by the lab of Joachim Wittbrodt might be just the set of tools that you need. The Golden GATEway cloning...which signaling events occur in a physiological setting. Genetic techniques typically rely on overexpression...RF-1; Bacterial strain EcAR7) and minimizing the set of plasmids required to make singly or multiply phosphorylated...between. The MoClo system is comprised of three sets of cloning vectors (Level 0, 1, or 2) which can ...Additionally, the lab recently described the use of another set of mGRASP plasmids in their Neuron 2014 paper. ...sequence to be integrated and a removable integrase cassette. They validated this methodology in two differents...expression and purification of Cas9 protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor...

...with the complete #MicrobeAdventCalendar ornament set (plus more!) It features archaea, bacteria, fungi...

... with GFP Kevin White’s lab recently deposited a set of CRISPR plasmids to tag human transcription factors...

... are sharing what they call “raw science” – data sets, code, and, I think it’s safe to say all of us here...

... kills you, but the cytokine storm it ultimately sets off.  The virus works its way into immune cells ...