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We narrowed to 616 results for: TIL

Showing: 161 - 180 of 616 results

  2. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...easiest way to make a knock-in cell line is to utilize the built-in repair pathway cells already have ... multiple rounds of cleavage at the target site until either the PAM site or part of the guide’s recognition...the most common editing pathway for DNA repair. To tilt the scales in your favor for an HDR edit, you can...

  3. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...ll prevent binding to any target protein that is still on the inside of the cell. However, assuming you...hidden epitopes. However, keep in mind that you will still contend with many of the same drawbacks to each ...early steps don’t even involve antibodies, they are still critical to the outcome of your IF experiment and...

  4. Tips for arabidopsis transformation

    Type
    Blog Post
    ... the base of the stem. This buys you a few days until new inflorescences begin to grow. This will also...too old to transform. This transformation could still work, but the efficiency will be low. Tips: Use...plants: If you transform early, your plants will still have plenty of time to produce seeds after you expose...

  5. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...available to researchers around the world. While we still offer many resources for producing vectors from ...vectors are not as easy to ship as plasmids, they still have a global reach. From 2016–2023, we shared 93,491... Rego, M., Wang, C., Zhang, Y., DeMaio, K. H., Tillgren, M., Fava, A., Clouse, G., Patrick, M., Guerin...

  6. Hot Plasmids - November 2023

    Type
    Blog Post
    ...post:  New compact, efficient prime editors A versatile and precise system to improve on-target delivery...10.1016/j.cell.2023.07.039. PMID: 37657419.   A versatile and precise system to improve on-target delivery...lab recently made two deposits that increase the utility of this technique.  By engineering a light-sensitive...

  8. Lab to Office Culture Shock

    Type
    Blog Post
    ...to recognize my transferable (or “soft”) skills until I was out of the lab and in an office environment...you your next job or promotion. Also, don’t wait until you’re out of the lab to think about these--get ...into science in the first place. Finally, if you still find the idea of not holding a pipette again upsetting...

  9. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...nucleic acids. Read on to learn more about this versatile technique.  Immunoprecipitation Overview An immunoprecipitation...typically carried out in one of two ways. Both methods utilize the same basic strategy: immobilize the antibody... One of the most common immobilization methods utilizes bacterial cell wall proteins, Protein A and Protein...

  10. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...generation of base modifiers available. This system utilizes an RNA template containing the desired edit adjoined...like with base editing, alternative Cas9s can be utilized allowing for PAM flexibility. Fig. 2: Prime...Don’t want to permanently modify the genome but still want to affect gene expression? dCas9 can be used...

  11. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...expressing it from a CAGGs promoter. However FLPe is still less efficient than Cre recombinase. FLPe codon ... Ligand regulated recombinase - keeping Cre out until needed But what can be done when the recombination...Cre/ FLP versions are retained in the cytoplasm until estrogen receptor ligand Tamoxifen is added. Tamoxifen...

  12. The Challenges of Cell Culture

    Type
    Blog Post
    ...technique is still used today to test for HeLa contamination. HeLa contamination is still a problem today...You would never thaw any of the Master cell vials until all of the working vials have been used up. This...

  13. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...many expression plasmids utilize inducible promoters, which are 'inactive' until an inducer such as IPTG...Arabinose-inducible expression of T7 RNAP; IPTG may still be required for expression F- ompT lon hsdSB (rB...

  14. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...the way we apprehend molecular complexes and is still a very useful tool.    Like a guitar hero (that ...SYFP2A, mTurquoise/mVenus and others. CFP-YFP is still one of the best and most used pairs to measure FRET... faster measurement systems, this technique has still many good days and good “gigs” ahead...No offense...

  15. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...something that has fallen out of fashion but is still useful and most importantly, effective. In the research...valuable tool for your research arsenal, and there is still a dedicated field of study for improving these tools... (2009). The RNA-induced Silencing Complex: a versatile gene-silencing machine. Journal of Biological ...

  16. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...can be achieved through two-protein labeling by utilizing(a) biotin-streptavidin binding and (b) antibody...are protected. Amine-binding viability dyes can still bind free amines on the cell surface, which yields...As a result, the largely negative live cells can still be easily gated on during the analysis. Conclusion...

  17. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...mutant, this technique uses mutagenic primers that tile across a region of interest, generating multiple...) at a given codon site and multiple primer sets tile across the protein or region of interest. A low ...). If so, the nicking mutagenesis protocol will still work if the orientation of the BbvCI site(s) of ...

  18. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...Escherichia coli One of the most recognizable and utilized model organisms is Escherichia coli. The bacterium... highly characterized genome, and widespread utilization, Arabidopsis has made significant contributions...number of offspring (produces many seeds), self fertilizing, a small yet highly characterized genome, and...

  19. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...track position 5 years after receiving their PhDs, still strive to become academic researchers themselves...universities to hire a glut of trainees at low wages will still be there without restructuring. Positive Points ...highlighting some of the important questions that still need to be answered about the makeup and welfare...

  20. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...F Host does (F') or does not (F-) contain the fertility plasmid. A low copy-number plasmid, encodes proteins...hsdR(rk-, mk+) Unmethylated DNA not degraded, cell still can methylate DNA   hsdS(rk-,mk-) Unmethylated...disruption of arabinose metabolism pathway Inability to utilize arabinose as a carbon source leuB β-isopropyl...
Showing: 161 - 180 of 616 results