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We narrowed to 795 results for: Tec

Showing: 161 - 180 of 795 results

  1. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...things more interesting, we are working in a new technical discipline that nobody understands (synthetic ... However, since the lab was highly focused on protecting intellectual property, it worked away in isolation...ideas (how to stop rhino horn poaching using biotechnology) were thrown on the table and dissected with...that survived the literature review, survived technical feasibility analysis, and was a marketable product... our day job, we worked on plants engineered to detect explosives and rapidly indicate their presence....reputation. The public is can be fearful of this technology and angry at the agriculture companies that produce...GMO food plants. When a press piece ran on the detector plants, we expected to see that same anger. Instead...

  2. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...enhanced detection of the product. As an added benefit, replicates are built into the technology. One well...applications for the technology.  Droplet digital PCR is well suited for the detection of low copy numbers... the detection of rare sequences and single cell analysis. It is also being used in the detection of microbes...approximately 20,000 droplets using water-oil emulsion technology. Each droplet contains the ingredients for amplification... Most droplet readers have a few channels for detecting fluorescence so that it is possible to measure...applications include measuring viable probiotics and detecting circulating pathogens.  With its many applications...Central PMCID: PMC3991984. Song, Neng, et al. "Detection of circulating Mycobacterium tuberculosis-specific...

  3. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...molecules is necessary for a detectable signal, limiting the sensitivity of the technique. One potential solution...It seems that there’s a new CRISPR advance or technique published every week! One of the newest applications...make this system truly advantageous. This new technique, developed in Thoru Pederson’s lab, brings us ...to mapping pairs of intrachromosomal loci. The technique successfully resolved loci with physical map distances...modifications and applications Ma et al. developed this technique using standard fluorescence microscopy, and are...extending the potential imaging time for this technique. The NIH Common Fund has made mapping the 4D nucleome...of genomic loci. Ma et al. anticipate that the technique will be applicable to studies of cell cycle progression...

  4. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...the capabilities of fluorescence-based techniques in biotechnology. Recent examples of advancements include...These dyes can also be attached to an antibody to detect various targets, such as proteins, nucleic acids..., which are pivotal in their application in biotechnology (Grimm & Lavis, 2022). These variations directly...and acceptors in their product descriptions, or technical manuals. Specific applications and matching dye...several factors. Firstly, brighter dyes enable detection of finer details by improving signal-to-noise ...designed for use in super-resolution microscopy techniques, such as Stimulated Emission Depletion (STED)...-fluorescent light when excited. While it is technically cell-permeable, its use in live cells can sometimes...

  5. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...correlated to behavior, the technique is relatively slow and is not suited to detect rapid changes in DA concentration...slices Demonstrate DA detection in a single neuron in vivo in Drosophila Detect DA dynamics in response...extracellular fluid (Darvesh et al., 2011). This technique is performed with a probe surrounded by a semipermeable... lab has developed dLight1, a molecular tool to detect and image DA in live animals (Patriarchi et al.... kinetics (10 ms on and 100 ms off), enabling detection of individual peaks of DA release.  Using dLight1... sensors are powerful new developments in the detection and measurement of neurotransmitters in various...fluorescent sensor enables rapid and specific detection of dopamine in flies, fish, and mice." Cell 174.2...

  6. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...found on immune cells. However, there are more techniques and strategies one can employ beyond simply incubating...downstream application, one or several of those techniques can be used alongside standard surface labels...introduce you to a few useful labeling and staining techniques beyond standard surface labeling. Pro tip! Many...and 'staining' interchangeably to refer to any technique that combines a marker with a signal. For clarity...labeling While antibodies used for target marker detection are usually conjugated to a fluorophore, not every...cases, a good alternative is using a two-protein detection system, as is commonly used in western blotting...proximity to the target marker, allowing for its detection. The advantage of this system is that streptavidin...

  7. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...especially in in vivo models, and new Cre-lox based technologies are still being developed today. In this post...differences amongst the Brainbow systems. Pre-Brainbow techniques, such as Golgi staining, injection of diffusible...time-intensive, necessitating the development of a new technique. In a structure as tightly packed and interwoven...name Brainbow is truly fitting for this colorful technology. Optimizing the system: Brainbow-3 Although Brainbow... et al. released a refinement of the Brainbow technology, Brainbow-3.0, with the goal of overcoming the...mutant YFP that does not fluoresce, but can be detected via immunostaining. This feature facilitates screening...Karine Loulier, et al. (2014). Currently, Brainbow techniques are also being applied to study model organisms...

  8. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...simple technology,” Doudna further explained. “That’s why we’re seeing adoption of the technology in different...University.  There can be no doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing...CRISPR for complex genetic diseases As CRISPR technology becomes increasingly more cost effective and ...CRISPR/Cas9 in comparison to other genome editing techniques is its ease of use.  “I was really excited when...of those off-target mutations when using this technology. When CRISPR therapies are being given in vivo... “A glance at genome editing with CRISPR-Cas9 technology.” Current genetics. https://doi.org/10.1007/s00294... Khatibi et al. 2021.  “CRISPR Genome Editing Technology and its Application in Genetic Diseases: A Review...

  9. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ... most cell types. But prime editing, a CRISPR technique developed in David Liu’s lab in 2019, has added...Nature, is a “search-and-replace” genome editing technique that mediates targeted insertions, deletions, ...Liu lab contributed an improved prime editing “architecture” that can be combined with any of the PE2-PE5...with improved stability Traditional sgRNAs are protected from cellular degradation by Cas9 binding, but...target site, but it cannot drive prime editing. To protect the 3′ end, the Liu lab appended RNA pseudoknots...mammalian cells: the small RNA-binding exonuclease protection factor La. They found that La, which is ubiquitously...DNA sequences with twin prime editing. Nature Biotechnology, 40(5), 731–740. https://doi.org/10.1038/s41587...

  10. Antibodies 101: Conjugation

    Type
    Blog Post
    ...Fluorophores Fluorophores allow you to detect your antibodies using techniques like fluorescence microscopy, flow...conjugate various labels to antibodies that produce detectable signals like light or color. In this post, we...substrates for each of these enzymes allow you to detect enzyme activity (and therefore your antibody) either... light produced by a chemical reaction, can be detected using specialized imaging instruments or X-ray...biotin conjugated antibodies good options for detecting low abundance targets. Check out our post on Biotinylation...mention. Oligonucleotides allow for sensitive detection of low abundance targets with significant multiplexing...JL, et al (2020) Proximity Ligation Assay for Detecting Protein-Protein Interactions and Protein Modifications...

  11. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...collection page. SARS-CoV-2 detection tools There have been many detection tools developed over the last...methods to use CRISPR to detect SARS-CoV-2. Other protocols and tools for detection have been made openly...Zheng’s lab had already developed nucleic acid detections tools based on Cas12a and Cas13  which could ...could be repurposed for SARS-CoV-2 RNA detection. Early in the pandemic, labs around the world have been modifying... modifying these tools for COVID-19 detection with the ultimate goal to develop low cost and quick point-of-care...that can be used in a one-step RT-qPCR reaction to detect SARS-CoV-2 nucleic acids. Mammalian targets During...2020) Open Sharing During COVID-19: CRISPR-Based Detection Tools. The CRISPR Journal 3:142–145. https://doi.org...

  12. Engaging with science and society at pgEd

    Type
    Blog Post
    ...Education Project (pgEd). Advances in genetic technologies and other biomedical innovations promise an ...society are to figure out the best way to shepherd technologies through thorny ethical issues, ensure that everyone... fruits of scientific research, and prevent technological advances from exacerbating existing inequalities...about genetics, both as individual consumers of technologies and as societal participants in the political...public health, science policy, and science and technology studies (STS). I also worked with a number of...field of interest. We often communicate in very technical and jargon-filled language in academic writing...out opportunities to communicate science to non-technical audiences. Many universities or cities have organizations...

  13. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ... can be a lot of work — and what if you want to detect more than one protein in your sample? Or what if...! Rather than start over, you can remove the detection antibodies from your membrane, allowing you to...stringent stripping methods. If you are planning to detect multiple proteins through several rounds of stripping...the strength of bands from different rounds of detection. Finally, stripping and reprobing works best with...blots that use chemiluminescent or fluorescent detection, like horseradish peroxidase (HRP) or Alexa Fluor...Fluor dyes, respectively. Chromogenic detection produces colored precipitates that permanently stain the...it. Ideally, dry your membrane once before any detection steps to avoid retaining residual antibodies. ...

  14. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...chromosomes. These long range DNA interactions are often detected with chromosome conformation capture (3C) based... of the drawbacks of 3C methods, but could only detect chromatin interactions at one location in the genome...CAPTURE 2.0, an updated version of CAPTURE that detects chromatin interactions at hundreds of loci at once... The chromatin “picture” is then developed by detecting these chimeric DNA fragments by PCR or next-generation...Cross-links are reversed so that DNA fragments can be detected by PCR or deep sequencing. A drawback of the...of CAPTURE 2.0 CAPTURE 2.0’s increased rate of detecting chromatin interactions opens the door for answering...proof-of-concept experiment to show that CAPTURE 2.0 could detect multiple chromatin interactions at once. Chromatin...

  15. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

    Type
    Blog Post
    ...the detection of RNA, dsDNA, and now ssDNA. By comparing the ability of Cas12 and Cas14 to detect SNPs...adaptive immune systems that provide bacteria protection against infectious agents. Several enzymes behind...fantastic add-on to a diagnostic toolkit, called DETECTR, which has been created by the authors. This kit...kit uses Cas12 and Cas13 to quickly detect the presence of infectious organisms (ex: SARS-CoV-2) and genetic...SNPs using the DETECTR kit, the authors showed that improved specificity of Cas14 enables high-fidelity ...high-fidelity SNP genotyping. The development of  Cas14-DETECTR will soon enable the quick and efficient diagnoses...resources on the Addgene Blog Learn more about DETECTR Browse all of the CRISPR blog posts Download our...

  16. MXS Chaining

    Type
    Blog Post
    ... into plasmid DNA. In high throughput cloning techniques, although the specific sequences of the genetic...proteins and the availability of powerful imaging technologies, the combination of multiple fluorescent protein...-throughput cloning. MXS Chaining is one such technique and has been used to produce complex fluorescent...constructs. These fluorescent reporters can be used to detect structure and protein localization, as well as ...show robust labeling, with strong expression and detection of each fluorophore at the respective subcellular...that the separate modules combined using these techniques are free of specific restriction enzyme recognition...High Throughput Cloning Strategies Method Technology Pros Cons References Golden Gate Type II restriction...

  17. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...implications of using these technologies, but also the rapidity of the technologies’ adoption by the scientific...amongst the Nobel prize winners for developing the technology. Though these case studies are “old,” they lead...significantly over the last decade. He describes the detection of gravitational waves--a story I was much less...around the world. The paper describing the waves’ detection had over 1,000 authors. The chapter on deep learning...learning addresses the dilemma of creating a technology that could eventually replace scientists themselves...Addgene’s role in the rapid dissemination of these technologies, proposing that “Addgene, with its easy, fast...CRISPR-Cas plasmids, has been a key driver of the technology.” As an Addgene vet of 12 years, I’m very familiar...

  18. Plasmids 101: Protein tags

    Type
    Blog Post
    ... to) purification, detection, solubilization, localization, or protease protection. Thus far Plasmids ...proteins, which are sometimes used as tags for detection; however, those are just one (admittedly large...overexpress and purify proteins can face any number of technical challenges depending on their protein of interest...one-step purification with glutathione, can also protect against proteolysis. That’s one form of instability...it’s a good one), then your first step towards detecting, immunoprecipitating, or purifying that protein...Stability Good for purification with glutathione; protects against proteolysis, but may reduce solubililty... improves solubility; good for antibody-based detection SUMO ~100 amino acid protein  12 Stability At...

  19. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    Blog Post
    ...thanks to these technologies but many questions remain. The use of other technologies such as optogenetics... cellular movements in the immune system. The techniques used here could be adapted to studying other ...labeled cells? 1. Epifluorescence microscopy This technique uses a fluorescence microscope where the light...excitation microscopy is a fluorescence imaging technique that allows imaging of living tissue up to about...from this volume. The consequence is that you can detect signal in thick specimens. See the video below ...monitor cellular movements in real time. This technique was first Introduced to immunology by three papers...papers in the journal Science in 2002. Now, this technique and the in vivo live-cell imaging (intravital ...

  20. A Primer on Optogenetics: Introduction and Opsin Delivery

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    Blog Post
    ...not to say that the technique doesn’t have its limitations or that other technologies need to be discarded...relatively cheap (compared to many other technologies and techniques in the sciences). But how exactly does...the history of neuroscience research we have a technology that allows us to show causality in neural circuits...interest. Despite its power and versatility, the technique is remarkably straightforward to learn and relatively...behavioral optogenetics in order to implement the technique in my lab at The Rockefeller University. In this...exactly do you want to use optogenetics for? The technique is non-trivial; you don’t want to spend months...stereotaxic surgery is a neuroscience research technique often used beyond just optogenetics. Successful...
Showing: 161 - 180 of 795 results