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We narrowed to 213 results for: acr.2

Showing: 161 - 180 of 213 results

  1. Addgene: The First Twenty Years

    Type
    Blog Post
    ...beginning Let’s take a quick flash back to 2004. “Shrek 2” was the most popular movie in theaters, the Razr ...support sharing of CRISPR constructs and information across the globe.  “Read the Addgene guide: https://www.addgene.org...comprised of 1302 plasmids, was shared with researchers across the globe. As the pandemic slowed, Addgene began...

  2. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ... PMID: 25184501. PubMed Central PMCID: MC4262738. 2. Doench, John G., et al. "Optimized sgRNA design to... method (Tanenbaum et al., 2014). The sgRNA or tracrRNA sequences can also be modified to include RNA ...transcription factors. The Root and Doench labs utilized tracrRNAs with two PP7 stem loops to recruit additional ...the targeted region (Sanson et al., 2018) . This tracrRNA was combined with the sgRNA expression vector ...

  3. Hot Plasmids - October 2020

    Type
    Blog Post
    ...products have distinct localizations. Figure 2: Split-wrmScarlet labeling proteins with different...-FT consistently predicted the cell cycle speed across these different cell types. In one example, H2B-FT...Find the lentiviral preps at Addgene hSyn1-SIO-stGtACR1-FusionRed is a Cre-dependent, soma-targeting inhibitory...

  4. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...Hoogsten base pairs and wobble pairs.  Figure 2: (A) The codon wheel depicts the 64 codons that encodes...translational efficiency is correlated with codon bias across all endogenous genes in E. coli and S. cerevisiae...electronic dataset for codon-usage of 257,468 genes across 8,792 organisms (Nakamura et al., 2000).  What ...to identify the ideal codon for every amino acid across an entire polypeptide. Many researchers have created...

  5. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused...coordinates optical stimulation of its associated fibers across multiple channels and can also record tissue temperature...

  6. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools...require when making such a decision remains spread across literature in publications that introduce these...

  7. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...start selecting and testing systemic capsids! Figure 2 contains a decision tree for selecting a capsid, while... highlighted below the table.     Figure 2: This decision tree can help guide your choice on ...transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt.3440 Goertsen...capsids, when writing this post, we didn’t come across a broadly used working definition of systemic capsids...expression (EGFP, in the depositing manuscript) across the brain. AAV-BI-28 has been tested in C57BL/6J...brain cells, with a higher efficiency (82 — 85% across the brain in mice) and greater specificity than...effectively and predominantly transduces neurons across brain regions, with reduced off-target expression...

  8. Hot Plasmids February 2024

    Type
    Blog Post
    ...overview of these new variants:   Figure 2: Inner mitochondrial membranes imaged with structured...X1.1 efficiently transduce brain endothelial cells across diverse mice strains and rats. Figure from Chen... et al. (2023). Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific...

  9. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...integrates into the trnS-leu2 locus.   Figure 2: Introducing Mobile-CRISPRi into bacteria. Top: A ...constitutely expressed RFP reporter, was variable across bacterial strains, ranging from an ~8 to 150-fold... reduction, with a median knockdown of ~40-fold across all species measured. Beyond understanding the ...

  10. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ... enrich the microvesicles containing AAVs (Figure 2). Since the development of the exo-AAV isolation protocol...secreted from producer cells also contain cellular macromolecules (e.g. proteins, nucleic acids) which possibly...

  11. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...create a recombinant antibody.     Figure 2: Changes to the heavy chain or light chain genes within...likely that this amino acid sequence will be shared across other proteins. Conversely, polyclonal antibodies...

  12. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...aspects of the work you presented may also be unclear. 2. Make the audience your primary focus When writing...graduate student leaders in science communication from across the US and Canada convene at ComSciCon's annual...

  13. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...account when simulating a digest as depicted in Lane 2. Controlling methylation Finally, you can control...Learn to Troubleshoot Your Sequencing Results Use REPLACR Mutagenesis to Mutate Your Plasmids Resources ...

  14. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...of concept for further development. Figure 2: Mechanisms of deaminase-based adenine transversion...refresher if you ever lose track of which acronym is which. Acronym Full name Notes   Base transition ...close at hand? Once again, this post has a lot of acronyms, so we've included a glossary at the end of this...polymerase known to preferentially incorporate A bases across from abasic sites. This approach resulted in a ... case, encouraging it to incorporate a new base across from the abasic site before filling the empty space...

  15. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...Nutr, 129 (2009a): 477–484. PubMed PMID: 10064313. 2. Chapman-Smith, A., & Cronan, J. E. “The enzymatic...your macromolecule of interest To utilize the natural binding of biotin and avidin, macromolecules of interest...process by which biotin is added to a protein or macromolecule. Biotin can be added enzymatically or chemically...soluble molecule that does not interfere with the macromolecules it is added to. Biotin also has an exposed ...developed several methods to chemically biotinylate macromolecules of interest both in vitro and in vivo. Biotinylation...

  16. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...Era of Personalized Medicine. Genes Dis. 2017 Jun;4(2):43-63. DOI: 10.1016/j.gendis.2017.04.001 Maetzig...adenoviruses are known to cause respiratory disease across species, as well as cancer in hamsters, although...

  17. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...PubMed PMID: 8491198. PubMed Central PMCID: PMC413431. 2. Bukrinsky MI, et al. “Active nuclear import of human...Finally, viral delivery is typically straightforward across applications, while electroporation of a human,...

  18. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ... high-priced commercial devices.     Fig. 2: Workflow for open software (left) vs. open hardware...communicating in science brings stronger networking across laboratory and disciplinary boundaries and can ...

  19. Hot Plasmids - May 2022

    Type
    Blog Post
    ... those that also infect the liver.    Fig. 2: Summary of the generational evolution of the AAV ...rate of less than 0.3%, both on and off target. Across all loci tested, error rates overall ranged from...

  20. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...and are considered “high copy”. A fragment from the 2 micron circle (a natural yeast plasmid) allows for.... coli cell, but vectors can be widely utilized across many different cell types and each one requires...
Showing: 161 - 180 of 213 results