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...laboratory research management consultant and coach in Boston, MA. He specializes in assisting research scientists...budget. Creating a budget We start by using your research specific aims. This is actually why a department...time it will take to secure your first (R-level) research grant.  Set your timeline from there, then start...talent pool that you’ll need to tap for your future research team. For example, if you’re going to need a tech...resources that you’ve thoroughly identified. Do online searches, and seek advice from your current administrative...information sites (like, indeed.com, or salary.com). Search industry and academic job titles in the regions...to identify which items aren’t critical to your research success. If you can get away with using computer...

...scientists writing research articles. What should you take into account when writing up your research? What type...subgroup of researchers in your area of expertise? Sometimes, the funders of your research may stipulate...more valuable to the research community. It also makes it more likely that researchers have enough information...Maaike Pols, PhD, a Developmental Editor at F1000Research.  With many changes taking place in the world...type of journal should I choose? Writing a research paper for publication in a scientific journal requires...increasing number of funding bodies insist that the research data resulting from their funding should also ...your Addgene plasmid #), and other reagents. F1000Research, among many other journals, has joined a pilot...

...typically writes the grants that fund your research, guides the research directions of the lab, gives you constructive...manuscript or fellowship reviewers, expose your research to search committees, and can lead to mutually beneficial...Student and Postdoctoral Programs at theJanelia Research Campus of the Howard Hughes Medical Institute....that attest to your talent and expertise as a researcher. Depending on the position, you may also want...during your postdoc At the end of grad school, you searched for and eventually identified a postdoctoral mentor...constructive feedback on your research, and teaches you how to write as well as mentor. Yet, even the most... least one more person that knows you and your research well. Who could fill these different roles? A ...

...provide them on your smart phone. 1. Lab Archives by LabArchives The single, most important part of lab ...instructional videos. The search function in the manual just highlights what the user searches, which is not very...heavily on adapting my smartphone to help me with my research in terms of preparedness, organization, and sharing... life. These apps prevent you from constantly searching the interwebs for lab resources, and instead conveniently...lab life is the lab notebook. Lab Archives is a multipurpose application that allows you to share your data...notebook from your smartphone to your computer. Lab Archives will help both you and your lab stay organized...from 2 years ago. The free subscription to Lab Archives allots for 25 MB of space per user, but, if you...

...methodologies and rigorous research plans. However, amidst the organized chaos of lab research and PhD studies, ... Widening your search The first step towards inviting serendipity into your research lies in expanding...structured world of lab research and PhD studies, where every aspect of research is meticulously planned... the potential to redefine the course of one's research journey — serendipity. The best scientific breakthroughs...enhancing your project's potential. Widening your search creates fertile ground for serendipitous encounters...become very focused on the details of a specific research question. However, breakthroughs often happen ...the constant stream of thoughts related to lab research and gain fresh perspectives. Such activities foster...

...parts of the world in my early research career. I've found that research institutes in different places...I believe that enChIP will markedly accelerate research of regulation mechanisms of genome functions in... enChIP, and I'm very happy to see that many researchers all over the world have requested plasmids for...cells. We believe that this system will allow researchers to use enChIP in a wide variety of biological...promote Addgene, which is also beneficial to my research. I believe that involvement in the scientific ...scientific community is a kind of duty for researchers when considering the peer-review system of journals and ...Addgene: Throughout your career, you have held research appointments in the US (New York University School...

...one visual barcode per cell and up to 200-plex with two barcodes per cell. The researchers validated their...visual barcodes for clonal multiplexing of cells in live imaging application. Each visual barcode consists...visual barcodes in use in A375 cell line. Image from Kaufman, et al, 2022.    This new barcoding technology... error rate for long sequence knock-ins Visual barcodes for live cell clonal multiplexing Improving ...interaction). The modularity of the system provides researchers better flexibility and makes the approach more...generate “single-use” transgenic lines. Instead, researchers can generate and maintain transgenic lines that...error rate. For a one kilobase insertion, the researchers reported an editing error rate of less than 0.3%...

...scientific community speed research is to offer plasmids created as part of research projects, another way ...Consortium collection enables research on solute carriers (SLCs) for medical research Recently, the Michael Davidson...Susanna Bachle and Matt Takvorian When COVID-19 researchers started working with us to help them share their...Transfer Offices at universities to make COVID-19 research-related plasmids available more broadly: to scientists...for-profit setting.  In light of the need for basic research during the current pandemic, universities are ... of support about allowing use of plasmids for research by anyone. As infection rates were increasing ...partners quickly assessed how to more readily enable research. Often “normal” timelines that could be weeks ...

...more into what researchers felt about NgAgo, I conducted a survey and asked researchers if they had tested...Update (November 18, 2016): Researchers from a variety of institutions recently reported their inability...sequence for target recognition, which gives a researcher unprecedented freedom to target any sequence ...form a complex with an ssDNA guide. Therefore, researchers must co-transfect cells with 5’-P-ssDNA guides... in the versatility of its delivery methods. Researchers can conveniently transfect/inject cells with ...requested by labs around the globe nearly 400 times. Researchers have been excitedly testing NgAgo's genome editing...discussions in the forums, it appeared that many researchers were having difficulty achieving indel formation...

...Universities and research institutions can turn to Addgene as a trusted partner for archiving their biological...present an unprecedented challenge to the scientific research ecosystem, not just in the United States but around...organization that directly collaborates with NIH-funded researchers and utilizes grants to support our own initiatives...life-saving medical breakthroughs and undermine critical research infrastructure that has taken decades to establish.These...remains steadfast in our mission to accelerate research and discovery by improving access to high-quality...funding. Addgene is uniquely positioned to help research institutions maximize their increasingly limited...scientific assets: We hold a repository of valuable research materials that might otherwise be lost amid lab...

... like many search engines, featuring both a general search bar tool and an advanced search option. For...   You can also search by protein sequence with BLAST, using the homepage search bar. Once you have...only Cas enzyme widely available and applied by researchers for gene targeting. Fast forward a decade, and...Institute. What is CasPEDIA? CasPEDIA is an online, searchable resource that provides summary information on...section on each enzyme page, you'll find a link to search Addgene's collection for plasmids containing that...  Helpful resources and information For some researchers, the previous information may be all you need...table of protein entry results. For the advanced search options, this is where you can really identify ...

...Balancing research with medical practice as an MD/PhD. Darrel does 20% clinical work 80% research and was... but later got obsessed with research. Chapter 3: How all basic research ends up being translational. ...for Regenerative Medicine accelerates research. Chapter 5: Research currently under way in the Kotton Lab...Chapter 8: The importance of a mix of research approaches. Why researchers should have a mix of high-risk, ...at Boston University Medical School. Darrell’s research focuses on the lungs, and, among other projects...Regenerative Medicine, and within the biomedical research community. Along these lines, Darrell recently...sharing. Listen to learn more about Darrell, his research, and his focus on open science. Listen to the...

...postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. It is commonly cited that...perform in vitro kinase reactions. Often, however, researchers lack knowledge of the regulatory kinase, access... phosphoserine (as described below) provides researchers with a valuable tool for understanding the contribution...curious anomaly amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA... phosphoserine (Sep)-tRNA synthetase enabled researchers to create an orthogonal translation system (OTS...encoding the ORF to be expressed Finally, the researcher needs a plasmid encoding their open reading frame...non-phospho-incorporated proteins purified from the system, researchers can run samples on SDS-PAGE gels containing Phos-Tag...

...has the sequence of the mutated gene the researcher is searching for), then the probe will bind to the sample...of DNA used saving the researcher both time and resources (3). Still, researchers generating transgenic...experiments allowed researchers to identify the location of genes. With this knowledge, researchers were able to...at the end as in standard PCR. This allows the researcher to compare different DNA sequences to see which...In the era before sequencing was commonplace, researchers used restriction mapping to investigate DNA sequence...running the products on an agarose gel allows a researcher to properly orient specific fragments of a DNA...This approach to studying DNA sequences helped researchers at a time when sequencing was still in the early...

...those by researchers hoping to increase community participation in new precision medicine research. A willingness...entails a lot of researching and writing – following the latest developments in genetics research, their applications...work in these fields of research, what often concerns them the most about research is its implications for...possibility of benefiting from the fruits of scientific research, and prevent technological advances from exacerbating...to dark episodes in the history of eugenics and research misconduct. Past ethical breaches such as the ...used to being very focused on narrowly-defined research questions in our field of interest. We often communicate...and awareness of issues outside of your field of research will allow you to better engage these diverse ...

...biomedical research: recommendations from the Future of Research Chicago Symposium F1000Research, 2016. Kyle...their careers (McDowell et al, F1000Research, 2014; Dolan, F100Research, 2016). These events have also...Shaping the Future of Research: a perspective from junior scientists."F1000Research 3 (2014). Pubmed PMID...Symposium and Workshop, organized by Future of Research, Academics for the Future of Science, and the ... More information can be found at http://futureofresearch.org/advocating-for-science-boston-2016/. Sign...Symposium Boston 2016 Changes since the Future of Research Symposia Ongoing challenges facing the scientific... 2014; Taylor, eLife, 2016). Several Future of Research (FoR) symposia held across the USA during this...

...reproducibility at some point. Usually it comes up when a researcher decides on a new project and begins by trying...variation in experimental conditions will derail a research program (think a subtle difference in mouse chow...sure you do what you can to help your fellow researchers advance science overall. Communication is the...inadequate information about what the original researchers actually did. Admittedly, the differences are...they come to light, it’s clear that what the researchers thought was a problem in fact was not, and it...in an accessible and consistent way so other researchers can use them, to making sure that methods and...to being open and willing to share with other researchers to help them troubleshoot and understand. All...

...required to study SARS-CoV-2, but with many academic research labs closed during the pandemic, it’s hard to ... are crucial for the advancement of SARS-CoV-2 research because they provide rapid access to validated... and much more have been critical for pandemic research. Image from Reproducibility for Everyone. Case...broad use and are now important for accelerating research into this virus. One such example is cell lines...repository for a variety of materials for life science research, including over 4,000 cell lines. Seventeen of...this study came from the Japanese Collection of Research Bioresources Cell Bank, part of the National Institutes...Nutrition in Japan. These results  will help researchers choose the best experimental model for further...

...Member and lead researcher at Imperial College London, Dr. Tom Ellis. Dr. Ellis does research in synthetic...chromosomes, and obstacles to becoming an academic researcher. Listen to the interview with Tom Ellis Podcast...breakdown Intro: How Tom ended up in academia and his research goals. From grad school to a startup and back ...in academia. Learning how to build a lab and a research program and difficulties with work-life balance... synthetic biology to the UK with foundational research, training, startups, and real applications of ..., disciplined, and open techniques. Chapter 4: Research projects underway in the Ellis lab including yeast...adoption of synthetic biology techniques in biology research as can already be seen with things like DNA synthesis...

... Genes:       BRG1(SMARCA4) BAF170 (SMARCC2) ARID1A   Chromatin research articles with plasmids...it’s true that adding histones and chromatin architecture to every diagram portraying some aspect of eukaryotic...when I recently attended the 2018 Chromosome Architecture and Chromosome Organization Keystone Symposium...but also specifically study it. They might be researching enhancer elements that control the transcription... or indirectly--many other areas of biological research. If you’re looking for resources that will help...posters I saw at the 2018 Keystone Chromosome Architecture and Chromosome Organization. These aren’t the...Kerstin Bystricky Courtney Hodges Sharon Dent Archa Fox Elaine Fuchs Kai Ge Robert Kingston Thoru ...