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We narrowed to 599 results for: arl

Showing: 161 - 180 of 599 results

  1. Viral Production at Addgene

    Type
    Blog Post
    ...immunogenic properties of the final product, particularly in large animal studies. Endotoxin contamination...detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). pAAV-CAG...being discarded and replaced with a new vial of early passage cells. Approximately two weeks post-thaw...

  2. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...could produce ultrasound contrast. That is, until earlier members of our lab - the Shapiro Lab - developed...applicability. They also did not scatter ultrasound nonlinearly, making them difficult to distinguish from background...next-generation bARGs that could scatter ultrasound nonlinearly and be easily expressed heterologously by bacteria...

  3. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ... interfering with downstream assays. This is particularly advantageous if the antibody used for IP is ...despite the size difference. Magnetic beads are particularly advantageous because they do not require centrifugation...binding proteins involved in histone modification. Similarly, RNA IP (RIP) can be used to isolate proteins ...

  4. Hot Plasmids - August 2020

    Type
    Blog Post
    ...markers tagged with mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within...CRISPRa) are great ways to modulate the expression of nearly any gene via specific guide RNA (gRNA) sequences... New from the viral service By Ina Ersing We regularly add new viral aliquots from our plasmid collection...

  5. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...products may be toxic to the host when expressed, particularly those that are insoluble, act on DNA, or are... can neither restrict nor methylate DNA. dcm: Similarly, strains with this mutation are unable to methylate...dead cells, and, conversely, you cannot induce too early as there are not enough cells in the culture to ...

  6. March for Science

    Type
    Blog Post
    ...inconvenient data must not be ignored. Returning to an earlier analogy, no amount of shouting will make an apple...comparison to 37% of the general public (5, 6). Similarly 90% of active research scientists believe climate...13th the March for Science homepage has amassed nearly 500,000 ‘likes’, while their website lists over...

  7. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...researchers investigating HIV integrase mutants in the early 1990s (Leavitt et al., 1996), and since then, numerous...severe combined immunodeficiency (SCID-X1) in the early 2000s. These trials unfortunately resulted in several...transduction.  The process for producing your own IDLVs is nearly identical to that of regular lentiviral vectors...

  8. Five Popular Model Organisms

    Type
    Blog Post
    ...transparent. Thus scientists can easily observe early developmental stages which can be difficult in other... large experiments over multiple generations. Similarly to other model organisms, the C.elegans genome... PubMed PMID: 26712014. Pandey, Udai Bhan, and Charles D. Nichols. "Human disease models in Drosophila...

  9. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...fluorescent and can be challenging to track intracellularly, but aptamers permit the study of intracellular...is better able to maintain its fluorescence intracellularly due to a lower dependency on magnesium and ...binding with RNA by about 7x). Mango fluoresces nearly as brightly as Spinach, but binds with much higher...

  10. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...research directions that were inaccessible with earlier tools. They also produce more than 20 other MTRIA...MTRIA biosensors are generated by inserting a circularly-permuted GFP into an intracellular loop of various...brightness, expression, and sensitivity compared with earlier SF-iGluSnFR. Image from Aggarwal 2022.   Find iGluSnFR3...

  11. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...improve muscle function, and they must be injected regularly. Dystrophin and genome editing Since complex oligonucleotide...approaches for exon skipping. Addgene depositor Charles Gersbach used paired zinc finger nucleases to remove...force, and other muscle tests, muscle function is clearly improved, although not to wild-type levels. With...

  12. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...) outside of the homology elements allowed for scarless insertion — no UTR sequence added to the genome...outside of the homology arms (red) to allow for scarless insertion of the payload (teal). The Cas9 gRNA...homology-directed repair (HDR), and insertion is scarless, unlike previous integrase-based approaches. Find...

  13. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...are always traveling, or too busy to interact regularly with the newer lab members. You must get an idea... the lab head? Are meetings with the lab head regularly scheduled or only ad hoc? Does the mentor keep...at your data and suggest follow up experiments regularly? Can you work on some experiments of your own ...

  14. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...the description to watch any sections you’re particularly interested in, but I’d recommend watching the...Reproducibility Project: Cancer Biology have produced similarly low rates of reproducibility. Failed attempts ...modified analyses on published data if you need to. Similarly, FigShare also makes it easier to share data through...

  15. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...mouse model generation simpler, as described in an earlier post on generating CRISPR mouse models. For simple...the example at right using blue flame plasmid pmScarlet_C1! After creating the T7-DNA template, you'd use...right homology arm to the desired insert (e.g., mScarlet).   Plasmid ID Plasmid Name Tags ...

  16. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...H3K27ac histone modification at specified loci. Charles Gersbach’s lab has deposited mammalian expression...pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1. LSD1 is a particularly large protein, and this fusion allows for packaging...this article. References Haldeman JM, Conway AE, Arlotto ME, et al (2018) Creation of versatile cloning ...

  17. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...Controlling the expression levels of your gene may be particularly useful when the gene being expressed has threshold...transposase/transposon gene modification system and the scarless, Footprint-Free™ removal of cargo with the excision-only...accessory transposon sequences.  The use of PBx is particularly important to avoid any possible random re-integration...

  18. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...and applied research since their first use in the early 90’s to genetically modify primary cells. Amongst...un-transfectable cells, labs use viral vectors and particularly retroviral vectors because they target a broader...was obvious that its safety was a disadvantage particularly for clinical development. In addition, MoMLV...

  19. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...reason most people enter postdoctoral training. Similarly, there are several factors to consider when selecting... get an experimental system up and running very early on. Once your system is working, you should maximize...to strengthen your new model?  PFs need to be regularly thinking about manuscripts. The other items will...

  20. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...base-pair size nearly indistinguishable from a Watson-Crick base pair, are joined with nearly the same efficiency...high-throughput profiling of ligase fidelity that extends earlier studies through a high-sensitivity multiplexed ...
Showing: 161 - 180 of 599 results