Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 582 results for: arl

Showing: 161 - 180 of 582 results

  1. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...researchers investigating HIV integrase mutants in the early 1990s (Leavitt et al., 1996), and since then, numerous...severe combined immunodeficiency (SCID-X1) in the early 2000s. These trials unfortunately resulted in several...transduction.  The process for producing your own IDLVs is nearly identical to that of regular lentiviral vectors...

  2. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...research directions that were inaccessible with earlier tools. They also produce more than 20 other MTRIA...MTRIA biosensors are generated by inserting a circularly-permuted GFP into an intracellular loop of various...brightness, expression, and sensitivity compared with earlier SF-iGluSnFR. Image from Aggarwal 2022.   Find iGluSnFR3...

  3. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...improve muscle function, and they must be injected regularly. Dystrophin and genome editing Since complex oligonucleotide...approaches for exon skipping. Addgene depositor Charles Gersbach used paired zinc finger nucleases to remove...force, and other muscle tests, muscle function is clearly improved, although not to wild-type levels. With...

  4. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...the description to watch any sections you’re particularly interested in, but I’d recommend watching the...Reproducibility Project: Cancer Biology have produced similarly low rates of reproducibility. Failed attempts ...modified analyses on published data if you need to. Similarly, FigShare also makes it easier to share data through...

  5. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...mouse model generation simpler, as described in an earlier post on generating CRISPR mouse models. For simple...the example at right using blue flame plasmid pmScarlet_C1! After creating the T7-DNA template, you'd use...right homology arm to the desired insert (e.g., mScarlet).   Plasmid ID Plasmid Name Tags ...

  6. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...H3K27ac histone modification at specified loci. Charles Gersbach’s lab has deposited mammalian expression...pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1. LSD1 is a particularly large protein, and this fusion allows for packaging...this article. References Haldeman JM, Conway AE, Arlotto ME, et al (2018) Creation of versatile cloning ...

  7. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...) outside of the homology elements allowed for scarless insertion — no UTR sequence added to the genome...outside of the homology arms (red) to allow for scarless insertion of the payload (teal). The Cas9 gRNA...homology-directed repair (HDR), and insertion is scarless, unlike previous integrase-based approaches. Find...

  8. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...are always traveling, or too busy to interact regularly with the newer lab members. You must get an idea... the lab head? Are meetings with the lab head regularly scheduled or only ad hoc? Does the mentor keep...at your data and suggest follow up experiments regularly? Can you work on some experiments of your own ...

  9. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...Controlling the expression levels of your gene may be particularly useful when the gene being expressed has threshold...transposase/transposon gene modification system and the scarless, Footprint-Free™ removal of cargo with the excision-only...accessory transposon sequences.  The use of PBx is particularly important to avoid any possible random re-integration...

  10. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...base-pair size nearly indistinguishable from a Watson-Crick base pair, are joined with nearly the same efficiency...high-throughput profiling of ligase fidelity that extends earlier studies through a high-sensitivity multiplexed ...

  11. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...and applied research since their first use in the early 90’s to genetically modify primary cells. Amongst...un-transfectable cells, labs use viral vectors and particularly retroviral vectors because they target a broader...was obvious that its safety was a disadvantage particularly for clinical development. In addition, MoMLV...

  12. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...reason most people enter postdoctoral training. Similarly, there are several factors to consider when selecting... get an experimental system up and running very early on. Once your system is working, you should maximize...to strengthen your new model?  PFs need to be regularly thinking about manuscripts. The other items will...

  13. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...that other technologies need to be discarded but clearly we are in an era of neuroscience research where...where optogenetics will be a standard tool used in nearly any neuroscience project or topic of interest. ...elsewhere (here and here for instance) [2], [3]. Karl Deisseroth of Stanford University , one of the key...

  14. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...brain along with high off-target expression, particularly in the liver, when administered intravenously...different efficacy and tropism between NHP species, particularly between New World and Old World monkeys. Selecting...but its neuron transduction encompasses more molecularly-defined neuronal subtypes and with less bias ... to AAV9 in the pigtail macaque brain slices. Similarly, AAV9-X1.1 outperformed the other capsids in the...

  15. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...search for "CRISPR", short-hand for Clustered Regularly Interspaced Short Palindromic Repeats, in Pubmed...the PAM sequence is immediately adjacent to the nearly-homologous target sites. The mere presence of additional...pluripotent stem cells differing exclusively at two early onset Parkinson Point mutations. Cell, 146(2), 318...

  16. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

    Type
    Blog Post
    ...Listen to Our Segment on mCyRFP1 Hot Plasmid 4: mScarlet - a new, bright, and monmeric red fluorescent ...fluorescent protein.  Listen to Our Segment on mScarlet Special thanks to our sound engineer/producer, Eddy Page...

  17. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ...been an important animal model for research, particularly genetics research, for decades. Its short life...during our stay there. Yet capturing the flies is nearly impossible, due to their small size and inconspicuous...

  18. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...brain tissue is injected with compounds that intracellularly spread away from the site of application and...transport has been observed for AAV1, -5, -8 (McFarland et al., 2009), and bidirectional transport for...Neuroscience 193, 249–258. PubMed PMID: 21782903. McFarland, N.R., Lee, J.S., Hyman, B.T., and McLean, P.J...

  19. Adenovirus Guide

    Type
    Guide
    ... divided into early (E1A, E1B, E2, E3, E4) and late (L1, L2, L3, L4, L5) regions. Early transcripts are...5 (Ad5) and have been produced by replacing the early and late adenoviral transcript regions between the...rAdV vectors, as well as the deletion of the other early gene regions, E2 and E4. These modifications aimed... when administered in vivo, however deletion of early genes also resulted in a reduction of viral vector...Adenoviral plasmid E2A, E2B, E3, E4 in trans Adenoviral early transcript regions involved in viral DNA replication...

  20. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...envelope vector pMD2.G This plasmid has been requested nearly 6,000 times (over 800 orders in 2017) and expresses...variety of cells and study their effects. To date, nearly 900 articles cite the use of pMD2.G obtained from...
Showing: 161 - 180 of 582 results