We narrowed to 310 results for: bon

1. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

   Type

   Blog Post

   ...disrupted interactions between Cas9 and the phophate backbone of the target DNA strand via mutations N497A, ...

2. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   ...things like butanol - very simple molecules - a few carbons and oxygens. Nothing too much more sophisticated...

3. Zhang Lab CRISPR Page

   Type

   Collection

   ...60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone cloning information:...helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop - Contains...resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and...luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase Vectors for...LacZ, plus Cre recombinase 60229 : sgRNA cloning backbone with Cre recombinase 60230 : sgRNA targeted to...adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described ...control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR This plasmid ...

4. Early Career Researcher Toolbox: Finding Relevant Papers

   Type

   Blog Post

   ...it’s also a good place to look for publications. Bonus: papers have to be summarized in 280 characters!...

5. Plasmids for Endogenous Gene Tagging in Human Cells

   Type

   Blog Post

   ...(about 2.7kb total)—is inserted into a plasmid backbone for delivery to the cells. As a precautionary ...

6. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

   Type

   Blog Post

   ...Encodes one insert, encodes gRNA/shRNA, or empty backbone. Purpose Answer “What does this plasmid ...

7. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

   Type

   Blog Post

   ...at the Faculty of Sciences of NOVA University in Lisbon, Portugal. She leads the Glycoimmunology group ...

8. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

   Type

   Blog Post

   ...only occur when galactose replaces glucose as the carbon source. Student results have shown that most, but...

9. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   ... make your own Fragmid components! Ensure your backbone and genes contain only the Bbsl cut sites needed...

10. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    ...100–115 mM = 0.7%–0.8% (v/v) Breaks disulfide bonds HCl Adjust pH to 6.7–6.8 Adjust pH ...

11. Hot Plasmids February 2024

    Type

    Blog Post

    ...primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely due to its long,...

12. Viral Vectors 101: Chemogenetics

    Type

    Blog Post

    .../10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez M, Hu X, Galvan ...

13. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    ...gRNA expression can be achieved using lentiviral backbones expressing blasticidin or puromycin resistance...

14. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    ...reader-effector proteins. b) Golden Gate assembly (backbones available at Addgene) with examples of potential...

15. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    ...eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins (RNPs) failed to induce off-target editing...

16. Plasmids 101: FLEx Vectors

    Type

    Blog Post

    ...Schnütgen F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck NB. A directional strategy for...

17. How-to: 5 Steps to a Great Panel Discussion

    Type

    Blog Post

    ...before the event... Choose a moderator with a backbone. It is nice to have a subject area expert, but...

18. Plasmids 101: Protein Expression

    Type

    Blog Post

    ... include ribosomes, RNA polymerase, tRNAs, ribonucleotides and amino acids. Cell-free expression systems...

19. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    ...diluted from 10X and containing both Hepes and bicarbonate) is brought to room temperature and placed in...

20. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type

    Blog Post

    ...Protein Timers Browse All Fluorescent Protein Empty Backbones Check Out Our Fluorescent Protein Guide ...