We narrowed to 312 results for: bon

1. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   ...exogenous sequences and regenerates a native peptide bond at the split site. Figure 1: The base editor...

2. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   ...mcb.8.5.2159 Galvan A, Raper J, Hu X, Paré J, Bonaventura J, Richie CT, Michaelides M, Mueller SAL, Roseboom...

3. Developing Lab Management Software for Biology

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   ...plasmid, 5-prime, CRISPR, growth strains, vector backbone, etc.) and you simply don't have enough context...

4. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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   ...CombiGEM-based assembly into the CombiGEM lentiviral vector backbone pAWp12 (Figure 2). Cloning from the pAWp28 storage...

5. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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   ...disrupted interactions between Cas9 and the phophate backbone of the target DNA strand via mutations N497A, ...

6. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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   ...things like butanol - very simple molecules - a few carbons and oxygens. Nothing too much more sophisticated...

7. Plasmids for Endogenous Gene Tagging in Human Cells

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   ...(about 2.7kb total)—is inserted into a plasmid backbone for delivery to the cells. As a precautionary ...

8. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   ...Encodes one insert, encodes gRNA/shRNA, or empty backbone. Purpose Answer “What does this plasmid ...

9. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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   ...at the Faculty of Sciences of NOVA University in Lisbon, Portugal. She leads the Glycoimmunology group ...

10. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    ...only occur when galactose replaces glucose as the carbon source. Student results have shown that most, but...

11. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    ...gRNA expression can be achieved using lentiviral backbones expressing blasticidin or puromycin resistance...

12. Making CRISPR Plasmids Using Fragmid

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    ... make your own Fragmid components! Ensure your backbone and genes contain only the Bbsl cut sites needed...

13. Antibodies 101: Stripping and Reprobing Western Blots

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    ...100–115 mM = 0.7%–0.8% (v/v) Breaks disulfide bonds HCl Adjust pH to 6.7–6.8 Adjust pH ...

14. Hot Plasmids February 2024

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    ...primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely due to its long,...

15. Viral Vectors 101: Chemogenetics

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    .../10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez M, Hu X, Galvan ...

16. Early Career Researcher Toolbox: Finding Relevant Papers

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    ...it’s also a good place to look for publications. Bonus: papers have to be summarized in 280 characters!...

17. Hot Plasmids and Viral Preps - March 2021

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    ...reader-effector proteins. b) Golden Gate assembly (backbones available at Addgene) with examples of potential...

18. Cpf1 Update: Comparison to Cas9 and NgAgo

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    ...eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins (RNPs) failed to induce off-target editing...

19. Plasmids 101: FLEx Vectors

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    ...Schnütgen F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck NB. A directional strategy for...

20. How-to: 5 Steps to a Great Panel Discussion

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    ...before the event... Choose a moderator with a backbone. It is nice to have a subject area expert, but...